Biologia plantarum 44:603-606, 2001 | DOI: 10.1023/A:1013763208472

Micropropagation of Cardiospermum Halicacabum

S. Babber1, K. Mittal1, R. Ahlawat1, T.M. Varghese1
1 Department of Botany, CCS Haryana Agricultural University, Hisar-, Haryana, India

The in vitro studies with Cardiospermum halicacabum indicated that the different explants, i.e cotyledon, hypocotyl, cotyledonary node, leaf, internode and node had the potential to produce calli on Murashige and Skoog (MS) medium supplemented with benzylaminopurine (BAP) and napthalene acetic acid (NAA). Calli of different explant origin showed variable growth responses on different BAP concentrations. The shoots were favourably formed from the calli of leaf and cotyledon explants. The maximum number of shoots were produced from calli subcultured on MS + BAP (17.8 µM). The roots were initiated on growth regulator free MS medium.

Keywords: benzylaminopurine; in vitro culture; medicinal plants; plantlets multiplication
Subjects: Cardiospermum halicacabum; in vitro culture, micropropagation, regeneration; medicinal plants, micropropagation

Published: December 1, 2001Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Babber, S., Mittal, K., Ahlawat, R., & Varghese, T.M. (2001). Micropropagation of Cardiospermum Halicacabum. Biologia plantarum, 44(4), 603-606. doi: 10.1023/A:1013763208472.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)

    • Open full article

    References

    1. Harikrishnan, K.N., Hariharan, M.: Direct shoot regeneration from nodal explants of Plumbago rosea Linn, a medicinal plant.-Phytomorphology 46: 53-58, 1966.
    2. Jaiwal, P.K., Gulati, A.: In vitro high frequency plant regeneration of a tree legume Tamarindus indica L.-Plant Cell Rep. 10: 569-573, 1991. Go to original source...
    3. Jyoti, M.: Selection and characterization of cadmium tolerant cells in Solanum melongena L. var. H-7.-Ph.D. Thesis. CCS Haryana Agricultural University, Hisar 1993.
    4. Kiran, S.: Study on micropropagation of Cassia roxburghii DC through in vitro culture technique.-M.Sc. Thesis. CCS Haryana Agricultural University, Hisar 1992.
    5. Matsuoka, H., Hinata, K.: NAA-induced organogenesis and embryogenesis in hypocotyl callus of Solanum melongena L.-J. exp. Bot. 30: 363-370, 1979. Go to original source...
    6. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue culture.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    7. Radhakrishnan, T., Murthy, T.G.K., Chandran, K., Bandyopadhyay, A.: Micropropagation in peanut (Arachis hypogaea L.).-Biol. Plant. 43: 447-450, 2000. Go to original source...
    8. Rajkumar, R.: Hormonal responses in shoot multiplication of Camellia sinesis.-Indian J. Plant Physiol. 38: 340-341, 1995.
    9. Rao, P.V.L., De, D.N.: Tissue culture propagation of tree legume Albizzia lebbek (L.) Benth.-Plant Sci. 51: 263-267, 1987.
    10. Singh, V.K., Chaturvedi, H.C.: Regenerating ability on in vitro grown jute species.-Indian J. Plant Physiol. 2: 307-309, 1997.
    11. Vinod, S.: In vitro studies on the effect of salinity on salt tolerant and sensitive genotypes of chickpea (Cicer arietinum L.).-Ph.D. Thesis. CCS Haryana Agricultural University, Hisar 1994.

    Return to the content