Biologia plantarum 45:441-444, 2002 | DOI: 10.1023/A:1016234104613

Ex Vitro Rooting of Micropropagated Shoots of Stackhousia Tryonii

N.P. Bhatia1, P. Bhatia1, N. Ashwath1
1 Primary Industries Research Centre, School of Biological and Environmental Sciences, Central Queensland University, Rockhampton, Australia

Micropropagated shoots of Stackhousia tryonii were exposed (individually or in combination) to indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), and 1-naphthalene acetic acid (NAA) at concentrations 1, 2 or 4 g dm-3 with the view to induce rooting under ex vitro conditions. The treated microshoots were grown in a mist room for four weeks and assessed for survival, rooting percentage, number of roots and root length. The results showed that IBA at 2 g dm-3 was most effective in inducing roots. Mixing of two or more auxins markedly reduced rooting percentage indicating antagonistic effects. The results demonstrated the potential of combining ex vitro rooting and hardening in one step, with view to reducing costs of multiplying plants via micropropagation.

Keywords: auxins; indole-3-acetic acid; indole-3-butyric acid; 1-naphthalene acetic acid; microshoots; tissue culture
Subjects: auxins; ex vitro rooting, micropropagated shoots; in vitro culture, ex vitro acclimation, rooting; in vitro culture, micropropagation; in vitro culture, nutrient media modification; Stackhousia tryonii

Published: September 1, 2002Show citation

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Bhatia, N.P., Bhatia, P., & Ashwath, N. (2002). Ex Vitro Rooting of Micropropagated Shoots of Stackhousia Tryonii. Biologia plantarum, 45(3), 441-444. doi: 10.1023/A:1016234104613.
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