Biologia plantarum 48:625-628, 2004 | DOI: 10.1023/B:BIOP.0000047165.52040.d0
Comparative Performance of Micropropagated and Seed-Grown Tomato Plants
- 1 Primary Industries Research Centre, School of Biological and Environmental Sciences, Central Queensland University, Rockhampton, Australia
Morphological, physiological, fruit yield and quality related traits were compared between the seed-grown and tissue-cultured plants of tomato (Lycopersicon esculentum Mill.) cv. Red Coat in a greenhouse. No significant differences were observed for any of the traits studied except for the number of leaves and branches, which were higher in the seed-grown plants than in tissue-cultured plants at the later stages of growth. No phenotypic abnormality of the tissue-cultured plants was observed suggesting that genetic fidelity of tissue cultured plants can be maintained if appropriate plant growth regulators are used with fewer member of subcultures in the multiplication medium.
Keywords: explant; genetic stability; Lycopersicon esculentum; morphogenesis; multiplication; photosynthetic rate; rooting; stomatal conductance; respiration rate
Subjects: acclimation from in vitro to ex vitro culture; auxins; citric acid; cuttings raised from in vitro, and ex vitro propagated or seed-grown plants; flower, gas exchange; fruit, gas exchange; gas exchange, micropropagated and seed-grown plants; growth analysis, plant development, biomass and yield enhancement; in vitro culture, acclimation, adaptation; in vitro culture, micropropagation; Lycopersicon esculentum; nutrient medium, B5 (Gamborg); nutrient medium, Murashige and Skoog; seed-grown and micropropagated plants; stomatal conductance, micropropagated and seed-grown plants; tomato, micropropagated and seed-grown plants
Published: December 1, 2004Show citation
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References
- Bajaj, Y.P.S., Dhanju, M.S.: Regeneration of plants from callus cultures of Napier grass (Pennisetum purpureum).-Plant Sci. Lett. 20: 343-345, 1981. Go to original source...
- Bhatia, P.: Regeneration, Micropropagation and Somatic Embryogenesis in Tomato. (Lycopersicon esculentum Mill.).-PhD Thesis. Primary Industries Research Centre, Central Queesland University, Rockhampton 2003.
- Chen, C.H., Stenberg, N.E., Ross, J.G.: Clonal propagation of big bluestem by tissue culture.-Crop Sci. 17: 847-850, 1977. Go to original source...
- Deng, L.P., Guo, Y.H., Jia, Y.K., Sheng, J.H.: Preliminary report on cultures of shoot tips of tomato and the yields of plants from culture.-Hereditas 10: 9-10, 1988.
- Diettrich, B., Mertinat, H., Luckner, M.: Reduction of water loss during ex vitro acclimatization of micropropagated Digitalis lanata clone plants.-Biochem. Physiol. Pflanz. 188: 23-31, 1992. Go to original source...
- Donnelly, D.J., Vidaver, W.E.: Pigment content and gas exchange of red raspberry in vitro and ex vitro.-J. amer. Soc. hort. Sci. 109: 177-181, 1984.
- Donnelly, D.J., Vidaver, W.E., Lee, K.Y.: The anatomy of tissue cultured red raspberry prior to and after transfer to soil.-Plant Cell Tissue Organ Cult. 4: 43-50, 1985. Go to original source...
- Grout, B.W.W., Aston, M.J.: Modified leaf anatomy of cauliflower plantlets regenerated from meristem culture.-Ann. Bot. 42: 993-995, 1978. Go to original source...
- Henke, R.R., Mansur, M.A., Constantin, M.J.: Organogenesis and plantlet formation from organ-and seedling-derived calli of rice (Oryza sativa).-Physiol. Plant. 44: 11-14, 1978. Go to original source...
- Hronková, M., Zahradníčková, H., Šimková, M., Šimek, P., Heydová, A.: The role of abscisic acid in acclimation of plants cultivated in vitro to ex vitro conditions.-Biol. Plant. 46: 535-541, 2003. Go to original source...
- Kozai, T. Acclimatization of micropropagated plants.-In: Bajaj, Y.P.S. (ed.): Biotechnology in Agriculture and Forestry 17. High-tech and Micropropagation I. Pp. 127-141. Springer-Verlag, Berlin 1991. Go to original source...
- Leela, S., Soneji, J.R., Bollamma, K.T.: Analyzing somaclonal variation in micropropagated bananas (Musa spp.).-In Vitro cell. dev. Biol. Plant 39: 551-556, 2003.
- Nikolić, R., Mitić, N., Nešković, M.: Evaluation of agronomic traits in tissue culture-derived progeny of birdsfoot trefoil.-Plant Cell Tissue Organ Cult. 48: 67-69, 1997. Go to original source...
- Pandey, R.M., Singh, S.P.: Field performance of in vitro raised papaya plants.-Indian J. Hort. 45: 1-2, 1988.
- Pospíšilová, J., Solárová, J., Čatský, J.: Photosynthetic responses to stresses during in vitro cultivation.-Photosynthetica 26: 3-18, 1992.
- Somasundar, M., Gostimsky, S.A.: Somaclonal variation in tomato tissue culture.-Crop Improvement 19: 92-96, 1992.
- Swartz, H.J., Galletta, G.J., Zimmerman, R.H.: Field perfor-mance and phenotypic stability of tissue culture-propagated strawberries.-J. amer. Soc. hort. Sci. 106: 667-673, 1981.
- Tyagi, R.K., Yusuf, A., Dua, P., Agrawal, A.: In vitro plant regeneration and genotype conservation of eight wild species of curcuma.-Biol. Plant. 48: 129-132, 2004. Go to original source...
- Venkatachalam, P., Geetha, N., Priya, P., Rajaseger, G., Jayabalan, N.: High frequency plantlet regeneration from hypocotyl explants of tomato (Lycopersicon esculentum Mill.) via organogenesis.-Plant Cell Biol. mol. Biol. 1: 95-100, 2000.
- Wetzstein, H.Y., Sommer, H.E.: Leaf anatomy of tissue-cultured Liquidambar styraciflua (Hamamelidaceae) during acclimatization.-Amer. J. Bot. 69: 1579-1586, 1982. Go to original source...
- Wu, L., Antonovics, J.: Zinc and copper tolerance of Agrostis stolonifera L. in tissue culture.-Amer. J. Bot. 65: 268-271, 1978. Go to original source...
- Zaman, A., Islam, R., Joarder, O.I.: Field performance and biochemical evaluation of micropropagated mulberry plants.-Plant Cell Tissue Organ Cult. 51: 61-64, 1997. Go to original source...
- Zimmerman, R.H.: Micropropagation of woody plants: post tissue culture aspects.-Acta Hort. 227: 489-499, 1988. Go to original source...