Biologia plantarum 52:642-647, 2008 | DOI: 10.1007/s10535-008-0125-1

Genetic diversity assessment in Portugal accessions of Olea europaea by RAPD markers

A. I. Cordeiro1, J. F. Sanchez-Sevilla2, M. C. Alvarez-Tinaut3, M. C. Gomez-Jimenez3,*
1 Departamento de Olivicultura, ENMP-INIAP, Elvas, Portugal
2 Departamento de Agricultura de Litoral, Laboratorio de Bioquímica, IFAPA-CICE-Junta de Andalucía, Cortijo de la Cruz, Churriana, Málaga, Spain
3 Departamento de Biología y Producción de los Vegetales, Área Fisiología Vegetal, Facultad de Ciencias, Universidad de Extremadura, Badajoz, Spain

Eighty seven olive (Olea europaea ssp. sativa L.) cultivar accessions from Portugal were characterized by means of randomly amplified polymorphic DNA (RAPD) markers. Of the 11 arbitrary 10-mer primers tested a total of 92 polymorphic bands were obtained, representing 87.6 % of the total amplification products. Twenty nine different genotypes were clearly discriminated. Differences were not found among the amplification profiles from different individuals of the same cultivar. All the genotypes could be identified by the combination of three primers: OPR-1, OPK-14 and OPA-1, seven genotype-specific markers being detected. Genetic relationships were estimated by the unweighted pair-group method with arithmetic averaging (UPGMA). The genetic analysis of the results showed a gradual distance between the various cultivars, making it difficult to identify well-differentiated phylogenetic groups, although two clusters were distinguishable with 35 % similarity, in addition to three independent branches with lower similarity: Galega, Tentilheira and Redondal. The dendrogram reflect some relationships for most of the cultivars according to the use of the fruit and ecological adaptation.

Keywords: olive cultivars; UPGMA
Subjects: cultivar and genotype differences; genetic diversity, biodiversity; Olea europaea; olive; polymerase chain reaction (PCR); random amplified polymorphic DNA (RAPD)

Received: December 12, 2006; Accepted: September 20, 2007; Published: December 1, 2008Show citation

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Cordeiro, A.I., Sanchez-Sevilla, J.F., Alvarez-Tinaut, M.C., & Gomez-Jimenez, M.C. (2008). Genetic diversity assessment in Portugal accessions of Olea europaea by RAPD markers. Biologia plantarum, 52(4), 642-647. doi: 10.1007/s10535-008-0125-1.
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