Biologia plantarum 54:329-333, 2010 | DOI: 10.1007/s10535-010-0057-4

Stimulation of antioxidative enzymes and polyamines during stripe rust disease of wheat

B. Asthir1,*, A. Koundal1, N. S. Bains2, S. K. Mann3
1 Department of Biochemistry, Punjab Agricultural University, Ludhiana, India
2 Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, India
3 Department of Plant Pathology, Punjab Agricultural University, Ludhiana, India

Content of polyamines and activities of antioxidative enzymes in response to stripe rust disease caused by Puccinia striiformis have been studied in two wheat (Triticum aestivum L.) cultivars PBW 343 (resistant) and HD 2329 (susceptible). Various infection stages ranging from traces to 100 % were collected. Infection leads to stimulation of peroxidase (POD), superoxide dismutase (SOD), catalase, diamine oxidase and polyamine oxidase activities along with increase in putrescine, spermidine and spermine content while ascorbate, tocopherol and chlorophyll content decreased in HD 2329 and no visible symptoms appeared in PBW 343. Histochemical localization pattern of POD and SOD activities revealed correlation with lignin deposition in cell walls.

Keywords: diamine oxidase; peroxidase; polyamine oxidase; Puccinia striiformis; Triticum aestivum
Subjects: ascorbate; catalase; chlorophyll a,b; diamine oxidase; peroxidase; polyamine oxidase; polyamines; Puccinia striiformis; reactive oxygen species (ROS); stripe rust disease; superoxide dismutase (SOD); tocopherol; Triticum aestivum; wheat

Received: June 6, 2008; Accepted: March 25, 2009; Published: June 1, 2010Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Asthir, B., Koundal, A., Bains, N.S., & Mann, S.K. (2010). Stimulation of antioxidative enzymes and polyamines during stripe rust disease of wheat. Biologia plantarum, 54(2), 329-333. doi: 10.1007/s10535-010-0057-4.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)

    • Open full article

    References

    1. Agarwal, S., Sairam, R.K., Srivastava, G.C., Meena, R.C.: Changes in antioxidant enzymes activity and oxidative stress by abscisic acid and salicylic acid in wheat genotypes. - Biol. Plant. 49: 541-550, 2005. Go to original source...
    2. Anand, T., Bhaskaran, R., Raguchander, T., Samiyappan, R., Prakasam, V., Gopalakrishnan, C.: Defence responses of chilli fruits to Colletotrichum capsici and Alternaria alternata. - Biol. Plant. 53: 553-559, 2009. Go to original source...
    3. Angelini, R., Bragaloni, M., Federico, R., Infantino, A., Porta-Puglia, A.: Involvement of polyamines, diamine oxidase and peroxidase in resistance of chickpea to Ascochyta rabiei. - J. Plant Physiol. 142: 704-709, 1993. Go to original source...
    4. Asthir, B., Duffus, C.M., Smith, R., Spoor, W.: Diamine oxidase is involved in H2O2 production in the chalazal cells during barley grain filling. - J. exp. Bot. 53: 677-682, 2002. Go to original source...
    5. Asthir, B., Spoor, W., Duffus, C.M.: Involvement of polyamines, diamine oxidase and polyamine oxidase in resistance of barley to Blumeria graminis f. sp. hordei - Euphytica 136: 307-312, 2004. Go to original source...
    6. Barth, C., Moeder, W., Klessig, D.F., Conklin, P.L.: The timing of senescence and response to pathogens is altered in the ascorbate-deficient mutant vitamin C-1. - Plant Physiol. 134: 1784-1792, 2005.
    7. Chance, B., Maehly, A.C.: Assay of catalases and peroxidases. - Methods Enzymol. 2: 764-775, 1955. Go to original source...
    8. Claiborne, A., Fridovich, I.: Purification of the o-dianisidine peroxidase from E. coli. - J. biol. Chem. 254: 4245-4252, 1979.
    9. Cowley, T., Walters, D.R.: Polyamine metabolism in barley reacting hypersensitively to the powdery mildew fungus Blumeria graminis f. sp. hordei. - Plant Cell Environ. 25: 461-468, 2002. Go to original source...
    10. El-Zahaby, H.M., Gullner, G., Kiraly, Z.: Effects of powdery mildew infection of barley on the ascorbate-glutathione cycle and other antioxidants in different host-pathogen interactions. - Biochem. cell. Biol. 85: 1225-1230, 1995. Go to original source...
    11. Gossett, D.R., Millhollon, E.P., Lucas, M.C: Antioxidant response to NaCl stress in salt-tolerant and salt-sensitive cultivars of cotton. - Crop Sci. 34: 706-714, 1994. Go to original source...
    12. Harrach, B.D., Fodor, J., Barna, B.: Changes of antioxidants following powdery mildew infection of near-isogenic barley lines carrying different resistance genes. - Acta biol. Szeged. 49: 91-92, 2005.
    13. Hendry, G.A.F., Price, A.H.: Stress indicators: chlorophylls and carotenoids. - In: Hendry, G.A.F., Grime, J.P. (ed.): Methods in Comparative Plant Ecology: A Laboratory Manual. Pp. 148-152. Chapman and Hall, London 1993. Go to original source...
    14. Hira, C.K., Kawatra, B.L., Sadana, B., Mann, S.K.: Laboratory Manual in Foods and Nutrition. - Punjab Agricultural University, Ludhiana 2000.
    15. Kanwischer, I., Porfirova, S., Bergmuller, E., Dormann, P.: Alterations in tocopherol cyclase activity in transgenic and mutant plants of Arabidopsis affect tocopherol content, tocopherol composition, and oxidative stress. - Plant Physiol. 137: 713-723, 2005. Go to original source...
    16. Kariola, T., Brader, G., Li, J., Palva, T.: Chlorophyllase I, a damage control enzyme, affects the balance between defense pathways in plants. - Plant Cell 17: 282-294, 2005. Go to original source...
    17. Kuzniak, E., Sklodowska, M.: Fungal pathogen-induced changes in the antioxidant systems of leaf peroxisomes from infected tomato plants. - Planta 22: 192-200, 2005. Go to original source...
    18. Liu, J.H., Kitashiba, H., Wang, J., Ban, Y., Moriguchi T.: Polyamines and their ability to provide environmental stress tolerance to plants. - Plant Biotechnol. 24: 117-126, 2007. Go to original source...
    19. Liu, Q., Yang, J.L., He, L.S., Li, Y.Y., Zheng, S.J.: Effect of aluminium on cell wall, plasma membrane, antioxidants and root elongation in triticale. - Biol. Plant. 52: 87-92, 2008. Go to original source...
    20. Marini, F., Betti, L., Scaramagli, S., Biondi, S., Torrigiani, P.: Polyamine metabolism is upregulated in response to tobacco mosaic virus in hypersensitive, but not in susceptible tobacco. - New Phytol. 149: 301-309, 2001. Go to original source...
    21. Marklund, S., Marklund, G.: Involvement of superoxide anion radical in the auto-oxidation of pyrogallol and a convenient assay for superoxide dismutase. - Eur. J. Biochem. 47: 169-174, 1974. Go to original source...
    22. Moerschbacher, B.M., Mendgen, K.: Structural aspects of defense. - In: Slusarenko, A., Fraser, R.S.S., Van Loon, L.C. (ed.): Mechanisms of Resistance to Plant Diseases. Pp. 231-237. Kluwer Academic Publishers, Dordrecht 2000. Go to original source...
    23. Moldenhauer, J., Moerschbacher, B.M., Van der Westhuizen, A.J.: Histological investigation of stripe rust (Puccinia striiformis f. sp. tritici) development in resistant and susceptible wheat cultivars. - Plant Pathol. 55: 469-474, 2006. Go to original source...
    24. Sache, I., De Vallavieille-Pope, C.: Comparison of the wheat brown and yellow rusts, for monocyclic sporulation and infection processes, and their polycyclic consequences. - J. Plant Pathol. 138: 55-65, 1993. Go to original source...
    25. Sasaki, K., Iwai, T., Miraga, S., Kuroda, K., Seo, S., Mitsuhara, I., Miyasaka, A., Iwamo, M., Ito, H., Matsui, H.: Ten rice peroxidases redundantly respond to multiple stresses including infection with rice plant fungus. - Plant Cell Physiol. 45: 144-152, 2004. Go to original source...
    26. Su, G.X., Bai, X.: Contribution of putrescine degradation to proline accumulation in soybean leaves under salinity. - Biol. Plant. 52: 796-799, 2008. Go to original source...
    27. Suzuki, N., Mittler, R.: Reactive oxygen species and temperature stresses: A delicate balance between signalling and destruction. - Physiol. Plant. 126: 45-51, 2006. Go to original source...
    28. Szczotka, Z.: Polyamine changes in (Quercus borealis Michx.) and (Quercus robur L.) seeds during ageing in controlled conditions. - Acta Physiol. Plant. 6: 127-135, 1984.
    29. Walters D.: Resistance to plant pathogens: possible roles for free polyamines and polyamine catabolism. - New Phytol. 159: 109-115, 2003. Go to original source...
    30. Yamakawa, H., Kamada, H., Satoh, M., Ohashi, Y.: Spermine is a salicylate-independent endogenous inducer in both tobacco acidic pathogensis-related proteins and resistance against tobacco mosaic virus infection. - Plant Physiol. 118: 1213-1222, 1998. Go to original source...

    Return to the content