Biologia plantarum - Latest articles
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Photosynthetic pigments, betalains, proteins, sugars, and minerals during Salicornia brachiata senescenceOriginal Papers
A. K. Parida, A. Kumari, A. Panda, J. Rangani, P. K. Agarwal
Biologia plantarum 62:343-352, 2018 | DOI: 10.1007/s10535-017-0764-1
Senescence is the last developmental stage in plants during which recycling of nutrients takes place from senescing organs to newly formed organs such as young leaves and developing seeds. In the present work, senescence induced alterations in mineral ions, chlorophylls, carotenoids, betacyanin, betaxanthin, proteins, amino acids, sugars, starch, and polyphenols were monitored in shoots of an extreme halophyte Salicornia brachiata. A sharp decline in the content of chlorophylls, carotenoids, and proteins in the shoot was noticed at middle and late stages of senescence in comparison with early stage. However, the content of betacyanin, betaxanthin,...
Over-expression of transcription factor GhWRI1 in upland cottonOriginal Papers
Z. J. Liu, Y. P. Zhao, W. Liang, Y. P. Cui, Y. M. Wang, J. P. Hua
Biologia plantarum 62:335-342, 2018 | DOI: 10.1007/s10535-018-0777-4
Transcription factors are involved in lipid metabolism, and in present study, the transcription factor WRINKLED 1 (GhWRI1) was cloned from Gossypium hirsutum L. cv. Coker 201 by reverse transcription (RT)-PCR and rapid amplification of cDNA ends. The Pro35S:WRI1 vector was constructed and transformed into upland cotton cv. Sumian 20 using the pollen tube pathway method. After PCR and Southern blot verification of the positive transgenic plants, T2 transgenic lines derived from T1 individuals with the insertion gene in a single copy were chosen for further dissection. Semi-quantitative and quantitative...
The role of tripartite interaction of calcium sensors and transporters in the accumulation of calcium in finger millet grainOriginal Papers
S. B. Kokane, R. K. Pathak, M. Singh, A. Kumar
Biologia plantarum 62:325-334, 2018 | DOI: 10.1007/s10535-018-0776-5
Finger millet (Eleusine coracana) is one of important crops, and its grains contain an exceptionally high content of calcium. In order to investigate the molecular mechanism by which it orchestrate the accumulation of Ca2+ during grain filling, some candidate genes encoding calcium transporters [calcium exchangers (CAX1, CAX3)] and sensors [calcineurin-B like (CBL4 and 10)], a CBL-interacting protein kinase (CIPK24), and calmodulin (CaM) were identified using transcriptomics and differential expression analysis in two genotypes of finger millet differing in grain calcium content. These transporters...
Characterization and primary functional analysis of Pinus densata miR171Original Papers
B. Z. Hai, Z. B. Qiu, Y. Y. He, M. M. Yuan, Y. F. Li
Biologia plantarum 62:318-324, 2018 | DOI: 10.1007/s10535-018-0774-7
The miR171 is a conserved microRNA (miRNA) family and has been shown to participate in plant growth and development. However, the precise function of miR171 in Pinus densata remains largely unclear. Mature miR171 sequence comparison reveals high similarity between Arabidopsis thaliana and P. densata and the pre-miR171 could fold into a characteristic stem-loop hairpin structure. Genes encoding GRAS (GAI-RGA-SCR) family transcription factors and actin binding protein were identified as targets of pde-miR171 using a modified RNA ligase mediated 5' rapid amplification of cDNA ends (RLM-RACE). Furthermore,...
Osmotic stress affects growth, content of chlorophyll, abscisic acid, Na+, and K+, and expression of novel NAC genes in contrasting rice cultivarsOriginal Papers
S. García-Morales, F. C. Gómez-Merino, L. I. Trejo-Téllez, L. Tavitas-Fuentes, L. Hernández-Aragón
Biologia plantarum 62:307-317, 2018 | DOI: 10.1007/s10535-017-0761-4
Osmotic stress causes a series of morphological, physiological, biochemical, and molecular changes that alters plant growth, development, and productivity around the globe. Phytohormones, nutrients, and transcription factors may induce adaptive responses to osmotic stress in plants. We evaluated the effect of osmotic stress induced by 18.5 % polyethylene glycol (PEG) or 100 mM NaCl on growth, content of abscisic acid (ABA), chlorophyll (Chl), sodium, and potassium, and the expression of multifunctional NAC transcription factors in rice cultivars (the salt-tolerant Cotaxtla and salt-sensitive Tres Ríos). The PEG and NaCl decreased shoot height...
Identification of genes associated with drought tolerance in barleyOriginal Papers
S. F. Abou-Elwafa
Biologia plantarum 62:299-306, 2018 | DOI: 10.1007/s10535-017-0765-0
Mapping of quantitative trait genes (QTGs) associated with drought related traits is essential for improving drought tolerance in crop species. In silico identification of candidate genes relies on annotation of critical QTGs to a variety of web resource-based datasets. The barley reference sequence was employed to map QTGs significantly associated with the proline accumulation and osmotic potential. Annotation of the critical QTGs contigs to the NCBI protein database identified 72 gene orthologs located on chromosomes 1H, 2H, and 7H, from which seven genes were identified as candidates. Expression analysis of all seven candidate genes revealed...
Identification of gene co-expression networks involved in cold resistance of Lilium lancifoliumOriginal Papers
Y. B. Yong, W. Q. Li, J. M. Wang, Y. Zhang, Y. M. Lu
Biologia plantarum 62:287-298, 2018 | DOI: 10.1007/s10535-017-0767-y
Low temperature can affect the growth and development of lily, limiting the application of commercial cultivars in outdoor. Lilium lancifolium is an important cold-resistant wild lily, but little is known about how L. lancifolium tolerates cold stress at the molecular level. In this study, we identified and characterized genes and transcription factors associated with cold stress in control plants and plants treated by 4° C for 1 - 24 h. The construction of a highest reciprocal rank-based gene co-expression network along with its partition into defined functional modules using Markov cluster algorithm resulted in identification of 30...
Identification of the genes involved in heterotrimeric G-protein signaling in mulberry and their regulation by abiotic stresses and signal moleculesOriginal Papers
C. Y. Liu, Y. Z. Xu, W. Fan, D. P. Long, B. N. Cao, Z. H. Xiang, A. C. Zhao
Biologia plantarum 62:277-286, 2018 | DOI: 10.1007/s10535-018-0779-2
Heterotrimeric guanine-nucleotide-binding proteins (G-proteins) play important roles in signal transduction and regulate responses to various stresses. Although G-protein signaling pathways have been extensively identified and characterized in model plants, there is little knowledge in non-model and especially in woody plants. Mulberry is an economically and ecologically important perennial tree, which is adaptable to many environmental stresses. In this study, we identified and cloned six G-protein genes including one Gα, one Gβ, two Gγ, one RGS (regulator of G-protein signaling protein) and one RACK1...
Identification of putative CONSTANS-like genes from the de novo assembled transcriptome of leekOriginal Papers
C. Liu, Q. Tang, C. Cheng, Y. Xu, Z. Yang, Z. Dai, J. Su
Biologia plantarum 62:269-276, 2018 | DOI: 10.1007/s10535-018-0778-3
Leek is an economically important vegetable. In model plants, the CONSTANS (CO) and CONSTANS-like (COL) genes play central roles in plant flowering modulation. However, none of leek CO homolog has been identified, because of limited gene resources obtained in this crop. Here, we reported the transcriptome analysis of leek, along with the identification of putative leek CONSTANS-like (COL) (ApCOL) genes. A total of 189 713 non-redundant transcripts were de novo assembled by using about 128.9 million clean sequence reads, of which, 48 621 were achieved for functional annotation. Thereafter, the...
NaPi/SX-RNase segregates as a functional S-RNase and is induced under phosphate deficiency in Nicotiana alataOriginal Papers
H. J. Rojas, C. Caspani, E. G. Escobar, R. Quiroga, A. Goldraij
Biologia plantarum 62:261-268, 2018 | DOI: 10.1007/s10535-018-0783-6
In plants, class III T2 RNases involves two groups of structurally similar proteins, but with different biological functions: S-RNases and non-S-RNases. S-RNases have been involved in self-incompatibility whereas non-S-RNases have been implicated in stress responses. Here we report a novel class III RNase termed NaPi/Sx-RNase, which works both in self-incompatibility and in response to phosphate deficiency. The NaPi/Sx-RNase gene was identified in roots of Nicotiana alata grown in the absence of inorganic phosphate. Phylogenetic analysis showed that NaPi/Sx-RNase was included within the class...
Activation of polyketide synthase gene promoter in Cannabis sativa by heterologous transcription factors derived from Humulus lupulusOriginal Papers
G. S. Duraisamy, A. K. Mishra, T. Kocábek, J. Matoušek
Biologia plantarum 62:250-260, 2018 | DOI: 10.1007/s10535-017-0766-z
Cannabis sativa, an annual herbaceous plant, produce wide variety of secondary metabolites among which delta-9-tetrahydrocannabinol (THC) is the most important one. The dissection of biosynthetic pathway(s) of this compound and its regulation by transcription factors (TFs) is an important prerequisite for efficient biotechnological manipulation of its secondary metabolome. A polyketide synthase (PKS) of C. sativa catalyzes the first step of cannabinoid biosynthesis, leading to the biosynthesis of olivetolic acid. Cloning and analysis of PKS promoter based on online PLACE, PlantCARE, and Genomatix Matinspector...
Wheat chromosome instability in the selfed progeny of the double monosomics 1Rv-1AOriginal Papers
O. G. Silkova, Y. N. Ivanova, E. A. Krivosheina, E. B. Bondarevich, L. A. Solovey, E. A. Sycheva, N. I. Dubovets
Biologia plantarum 62:241-249, 2018 | DOI: 10.1007/s10535-017-0757-0
Structural alterations of chromosomes are often found in wheat-rye hybrids. In the majority of cases modifications are observed for rye chromosomes, yet chromosome aberration cases are described for wheat, including the progeny of Triticum aestivum disomic and monosomic addition lines. Since wheat-rye substitution and translocation lines are the source of rye chromatin in wheat breeding programs, the information on possible chromosome changes in the genomes of introgressive forms is important. Chromosome behavior in F1 meiosis and chromosomal composition of F2 karyotypes for double monosomics 1Rv-1A were studied by applying...
The complete chloroplast genome sequence of Pseudoroegneria libanotica, genomic features, and phylogenetic relationship with Triticeae speciesOriginal Papers
D. D. Wu, L. N. Sha, C. Tang, X. Fan, Y. Wang, H. Y. Kang, H. Q. Zhang, Y. H. Zhou
Biologia plantarum 62:231-240, 2018 | DOI: 10.1007/s10535-017-0759-y
Pseudoroegneria libanotica is an important herbage diploid species possessing the St genome. The St genome participates in the formation of nine perennial genera in Triticeae (Poaceae). The whole chloroplast (cp) genome of P. libanotica is 135 026 bp in length. The typical quadripartite structure consists of one large single copy of 80 634 bp, one small single copy of 12 766 bp and a pair of inverted regions (20 813 bp each). The cp genome contains 76 coding genes, four ribosomal RNA and 30 transfer RNA genes. Comparative sequence analysis suggested that: 1) the 737 bp deletion in the cp of P. libanotica was...
Identification of MYB transcription factor genes and their expression during abiotic stresses in maizeOriginal Papers
Y. H. Chen, Y. Y. Cao, L. J. Wang, L. M. Li, J. Yang, M. X. Zou
Biologia plantarum 62:222-230, 2018 | DOI: 10.1007/s10535-017-0756-1
The MYB transcription factor superfamily is a large gene family that plays central roles in developmental processes and defence responses in plants. Unlike in Arabidopsis, only few members of the R2R3-MYB gene family have been functionally well characterized in maize, especially in abiotic stress-response pathways. Subgroup-specific conserved motifs outside the MYB domain may reflect functional conservation. A comparative genomics study using Arabidopsis abiotic stress-responsive MYB protein sequences identified 46 ZmMYB genes that may be involve in abiotic stress responses of Zea mays. An expression...
Anatomy and photosystem II activity of in vitro grown Aechmea blanchetiana as affected by 1-naphthaleneacetic acidOriginal Papers
J. P. R. Martins, L. C. A. Rodrigues, E. R. Santos, B. G. Batista, A. B. P. L. Gontijo, A. R. Falqueto
Biologia plantarum 62:211-221, 2018 | DOI: 10.1007/s10535-018-0781-8
Auxins are one of the main regulators of in vitro plant growth and development. However, the mechanisms, by which auxins, such as 1-naphthaleneacetic acid (NAA), affect in vitro root and leaf anatomy and photosystem function, remain unclear. Accordingly, the aim of the present study was to analyze the effect of different NAA concentrations on the anatomy and photosynthetic performance of in vitro-propagated Aechmea blanchetiana and to determine whether such a treatment affects micropropagated plants after acclimatization. In vitro-established A. blanchetiana plants were transferred to culture media that contained...
Regulation of the key antioxidant enzymes by developmental processes and environmental stresses in the darkReviews
P. Poór, A. Ördög, Z. Czékus, P. Borbély, Z. Takács, J. Kovács, I. Tari
Biologia plantarum 62:201-210, 2018 | DOI: 10.1007/s10535-018-0782-7
The presence of solar radiation is one of the most important environmental factors, which is required for the optimal growth and development of plants. The absence of it (e.g. in the night period or artificially prolonged darkness) can alter the light-dependent signalling and regulation pathways and may induce new defence responses. Antioxidant enzymes as components of the plant defence system play a crucial role in the detoxification of reactive oxygen species (ROS) induced by several stressors; however, their regulation can be different in the light or in the dark. In this review we summarize the current knowledge about the physiological and...
Photoperiod and ethylene-dependent expression of gibberellin biosynthesis gene InEKO1 during flower induction of Ipomoea nilBrief Communications
K. Marciniak, E. Wilmowicz, A. Kućko, J. Kopcewicz
Biologia plantarum 62:194-199, 2018 | DOI: 10.1007/s10535-017-0743-6
Ent-kaurene oxidase (EKO) catalyze three sequential oxidations in the early steps of gibberellin biosynthesis pathway. In this research, a cDNA sequence of InEKO1 gene in the model short-day plant Ipomoea nil was identified. Our studies revealed that inductive conditions for flowering caused an increase in the transcriptional activity of the examined gene in the cotyledons-the main organs for the perception of the photoperiodic stimulus. In contrast, in the second half of the 16 h long inductive night and after that, a decreased amount of InEKO1 mRNA in the apexes was detected. What is more, ethylene, the key inhibitor of...
Hydrogen sulfide is involved in the regulation of ascorbate and glutathione metabolism by jasmonic acid in Arabidopsis thalianaBrief Communications
C. Shan, T. Wang, Y. Zhou, W. Wang
Biologia plantarum 62:188-193, 2018 | DOI: 10.1007/s10535-017-0740-9
This study investigated the role of hydrogen sulfide (H2S) in the regulation of the ascorbate (AsA) and glutathione (GSH) metabolism by jasmonic acid (JA) in the leaves of Arabidopsis thaliana by using H2S scavenger hypotaurine (HT) and H2S synthetic mutant (SALK_041918, designated Atl-cdes). The results showed that JA significantly increased the H2S content, the activities of L-cysteine desulfhydrase (L-CDes), D-cysteine desulfhydrase (D-CDes), ascorbate peroxidase (APX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase...
Allantoin accumulation in response to increased growth irradiance in Arabidopsis thalianaOriginal Papers
S. Irani, J. M. Lobo, G. R. Gray, C. D. Todd
Biologia plantarum 62:181-187, 2018 | DOI: 10.1007/s10535-017-0747-2
Excess radiation is one of frequent natural environmental stresses that plants have to cope with on a daily basis. Therefore, plants have evolved many short- and long-term mechanisms to acclimate to high irradiance and tolerate it. Ureides, generated from purine degradation, have been proposed as compounds involved in environmental stress responses, including altered irradiance. In the present study, high irradiance was used to investigate ureide content and gene expression in Arabidopsis thaliana. Arabidopsis plants shifted to high irradiance showed high content of a specific ureide compound, allantoin. The accumulation of allantoin...
Changes in leaf tissue of Carica papaya during single and mixed infections with Papaya ringspot virus and Papaya mosaic virusOriginal Papers
M. A. García-Viera, L. Sánchez-Segura, G. Chavez-Calvillo, D. Jarquín-Rosales, L. Silva-Rosales
Biologia plantarum 62:173-180, 2018 | DOI: 10.1007/s10535-017-0741-8
Papaya (Carica papaya L.) is susceptible to viral diseases caused by Papaya mosaic virus (PapMV) and Papaya ringspot virus (PRSV), which limit fruit production and affect economic yield. The symptoms produced by both the viruses are similar in early stages of infection and include vein and leaf chlorosis, which develop into mosaic at later stages of infection when leaf lamina can get reduced in size and distorted with a shoe-string aspect. Digital image analyses, such as fractal dimension (FD) and lacunarity (λ) were used here to examine papaya tissue after single and mixed infections of PRSV and PapMV. Morphological changes,...
The effects of silver ions and silver nanoparticles on cell division and expression of cdc2 gene in Allium cepa root tipsOriginal Papers
A. S. Fouad, R. M. Hafez
Biologia plantarum 62:166-172, 2018 | DOI: 10.1007/s10535-017-0751-6
The effects of silver nanoparticles (AgNPs), silver ions (Ag+), and polyvinylpyrrolidone (PVP) on mitosis and expression of a gene encoding cyclin-dependent kinase 2 (cdc2) in onion roots were compared. Three concentrations (5, 10, and 15 mg dm-3) were employed in combination with three incubation times (3, 6, and 9 h). PVP enhanced mitotic index and cdc2 expression. Both silver forms decreased mitotic index and cdc2 expression. Genotoxicity of both silver forms were indicated by three major distinguishable classes of chromosome aberrations: spindle disturbances, clastogenic aberrations, and chromosome stickiness....
Xylem sap chemistry: seasonal changes in timberline conifers Pinus cembra, Picea abies, and Larix deciduaOriginal Papers
A. Losso, A. Nardini, B. Dämon, S. Mayr
Biologia plantarum 62:157-165, 2018 | DOI: 10.1007/s10535-017-0755-2
The seasonal course of xylem sap parameters (electrical conductivity EC, potassium concentration [K+], and pH) of three conifers (Pinus cembra, Picea abies, and Larix decidua) growing at the alpine timberline was monitored. We also looked into possible effects of [K+] and pH on the difference in hydraulic conductivity (Δks). In all studied species, EC, [K+], and pH varied considerably over the year, with pH ranging between 7.3 (February) and 5.8 (June) and [K+] changing between 0.4 (January) and 2.5 mM (June). The Δks was overall low with positive values...
Comparison of nitrogen uptake in the roots and rhizomes of Leymus chinensisOriginal Papers
S. Li, F. Jiang, Y. Han, P. Gao, H. Zhao, Y. Wang, S. Han
Biologia plantarum 62:149-156, 2018 | DOI: 10.1007/s10535-017-0748-1
Leymus chinensis (Trin.) Tzvel is a rhizomatous grass species in the Eastern Eurasian steppe zone that is often limited by low soil nitrogen availability. Although a previous study showed that the rhizomes of L. chinensis have the capacity to take up nitrogen, the importance of such uptake for nitrogen nutrition is unclear. Moreover, little is known regarding the inorganic nitrogen uptake kinetics of roots and rhizomes in response to nitrogen status. Here, we first found that ammonium is preferred over nitrate and glycine for L. chinensis growth. Using the 15N-labelling method, we found that the rate of ion influx into...
Freezing tolerance of chickpea: biochemical and molecular changes at vegetative stageOriginal Papers
Ö. Arslan, F. Eyidoğan, Y. Ekmekçi
Biologia plantarum 62:140-148, 2018 | DOI: 10.1007/s10535-017-0760-5
The aim of this study was to find a correlation between the freezing tolerance of three chickpea (Cicer arietinum L.) cultivars (İnci, Işik-05, and Sari-98) and their wild relative C. echinospermum and physiological responses. Chickpea plants (15-d-old) were subjected to cold acclimation (CA) (10 °C for 7 d), freezing (-3 or -5 °C for 2 h), and subsequent rewarming (25 °C for 7 d). In two separate experiments with three replications, we determined growth, water status, photosystem 2 photochemical activity, photosynthetic pigments, H2O2, malondialdehyde, and proline content, relative leakage ratio, antioxidant...
Tolerance to soil water stress by Oryza sativa cv. IR20 was improved by expression of Wsi18 gene locus from Oryza nivaraOriginal Papers
R. Kaur, A. Chakraborty, R. K. Bhunia, S. K. Sen, A. K. Ghosh
Biologia plantarum 62:129-139, 2018 | DOI: 10.1007/s10535-017-0742-7
Wild rice genotypes are rich in genetic diversity. This has potential to improve agronomic rice by allele mining for superior traits. Late embryogenesis abundant (LEA) proteins are often associated with desiccation tolerance and stress signalling. In the present study, a group 3 LEA gene, Wsi18 from the wild rice Oryza nivara was expressed under its own inducible promoter element in stress susceptible cultivated indica rice (cv. IR20). The resulting transgenic plants cultivated in a greenhouse showed enhanced tolerance to soil water deficit. Transgenic plants had higher grain yield, plant survival rate, and shoot relative...
The effect of boron availability, CO2, and irradiance on relative accumulation of the major boron transport proteins, BOR1 and NIP5;1Original Papers
S. Mishra, S. A. Heckathorn, J. M. Frantz, C. Krause
Biologia plantarum 62:121-128, 2018 | DOI: 10.1007/s10535-017-0744-5
Boron (B) is an essential plant micronutrient. Two major B-transport proteins have been recently identified and partially characterized: BOR1, a high-affinity B efflux transporter involved in xylem loading, and NIP5;1, a plasma-membrane boric-acid channel involved in B uptake. To date, studies of these B transporters have investigated their expression individually (mainly as mRNA), and only in response to variation in B availability (mostly B deficiency); the influence of other factors, such as plant resource status, has not been studied. To address this, we grew geranium (Pelargonium × hortorum cv. Maverick White) plants under ambient...
Characterization, genetic diversity, phylogenetic relationships, and expression of the aluminum tolerance MATE1 gene in Secale speciesOriginal Papers
E. Santos, C. Benito, J. Silva-Navas, F. J. Gallego, A. M. Figueiras, O. Pinto-Carnide, M. Matos
Biologia plantarum 62:109-120, 2018 | DOI: 10.1007/s10535-017-0749-0
Aluminum (Al) is the main limiting factor for crop production in acidic soils. Efflux of organic acids is one of the mechanisms that determine Al-tolerance, and an Al-activated citrate transporter (multidrug and toxic compound extrusion) MATE1 gene is involved in different species. The contribution of the rye MATE1 gene (ScMATE1) depends on the rye (Secale cereale L.) cultivars and the crosses analyzed; there is no information about different rye species. The cDNA sequences, phylogenetic relationships, Al-tolerance, citrate exudation, and expression of the ScMATE1 gene were analyzed in several cultivars and wild species/subspecies...
Identification and comparative analysis of aluminum-induced microRNAs conferring plant tolerance to aluminum stress in soybeanOriginal Papers
S. C. Huang, G. H. Lu, C. Y. Tang, Y. J. Ji, G. S. Tan, D. Q. Hu, J. Cheng, G. H. Wang, J. L. Qi, Y. H. Yang
Biologia plantarum 62:97-108, 2018 | DOI: 10.1007/s10535-017-0752-5
Aluminum (Al) toxicity in acidic soils is a major factor restricting crop production. Although the molecular mechanisms of Al responses have been extensively investigated, microRNA (miRNA) mediated differential Al tolerance in different soybean genotypes remains largely unknown. In this study, two soybean [Glycine max (L.) Merr.] genotypes, Al-tolerant BX10 and Al-sensitive BD2, were treated with 0 and 50 μM AlCl3 and then used to construct the miRNA libraries for deep sequencing. Results revealed 453 miRNAs, whose expression patterns were affected by Al stress. We also identified 32 differentially expressed miRNAs: 19 in BX10, 7 in BD2,...
Assessment of reference genes for real-time quantitative PCR normalization in Ilex paraguariensis leaves during droughtOriginal Papers
R. M. Acevedo, E. H. Avico, O. A. Ruiz, P. A. Sansberro
Biologia plantarum 62:89-96, 2018 | DOI: 10.1007/s10535-017-0754-3
Reverse transcription of RNA followed by real-time quantitative PCR (qPCR) is to date the most reliable method for gene expression studies. However, to control the errors introduced along the numerous experimental procedures, it requires a normalization using internal reference genes with stable expression. To address this issue, nine candidate reference genes were investigated in Ilex paraguariensis leaves subjected to water stress. To facilitate the selection, we analysed the real-time qPCR data with three different software programs. The obtained results support the conclusion that RNA polymerase associated protein rtf1 homolog (RTF)...
Knockout mutants of Arabidopsis thaliana β-galactosidase. Modifications in the cell wall saccharides and enzymatic activitiesOriginal Papers
M. Moneo-Sánchez, L. Izquierdo, I. Martín, J. Hernández-Nistal, L. Albornos, B. Dopico, E. Labrador
Biologia plantarum 62:80-88, 2018 | DOI: 10.1007/s10535-017-0739-2
This work studied the six β-galactosidases (BGALs) of the subfamily a1 of Arabidopsis, that have been proposed to play important roles in the cell wall remodelling during plant development, although their precise functions are still unknown. Knockout mutants bgal1, bgal2, bgal3, bgal4, bgal5, and bgal12 of Arabidopsis and their wild type (WT) plants were analysed to determine their morphology and composition of their cell walls. The gas chromatography and the Fourier transform infrared spectroscopy revealed differences between the mutants and their WT such as in the proportions of glucose,...