Biologia plantarum 55:783, 2011 | DOI: 10.1007/s10535-011-0188-2

Effects of hypobaric growth conditions on morphogenic potential and antioxidative enzyme activities in Saussurea involucrata

B. Guo1, B. H. Abbasi2, Y. H. Wei1,*
1 Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education; School of Life Science, Norhtwest University, Xi'an, P.R. China
2 Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan

Effects of reduced atmospheric pressure on morphogenic potential and antioxidative enzyme activities in regenerated tissues of Saussurea involucrata were evaluated. Leaf explants were cultured at atmospheric pressure 30, 60 or 101 kPa on Murashige and Skoog (MS) medium with several plant growth regulators (PGRs). Oxygen and carbon dioxide partial pressures were maintained at 21 and 0.038 kPa, respectively. At 60 kPa, 12 shoots per explant were recorded, which was 1.5 and 2.1-folds higher than at 101 and 30 kPa, respectively. A shooting frequency of 80 % was observed at 60 and 101 kPa. Rooted plantlets were obtained on MS medium with indoleacetic acid. At 30, 60 and 101 kPa, rooting of shoots was 49, 72 and 85.6 %, respectively. The rooted plantlets were successfully acclimatized to soil. Activities of all of antioxidative enzymes determined in present study were affected by hypobaric conditions.

Keywords: auxin; catalase; peroxidase; shoot organogenesis; superoxide dismutase

Received: June 11, 2010; Accepted: December 14, 2010; Published: December 1, 2011Show citation

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Guo, B., Abbasi, B.H., & Wei, Y.H. (2011). Effects of hypobaric growth conditions on morphogenic potential and antioxidative enzyme activities in Saussurea involucrata. Biologia plantarum, 55(4), 783. doi: 10.1007/s10535-011-0188-2.
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