Biologia plantarum, 1980 (vol. 22), issue 3

Article

Book reviews

Jiřina Slavíková

Biologia plantarum 1980, 22:191 | DOI: 10.1007/BF02892737

Book reviews

A. Kotyk, I. Dvořák, Jarmila Solárová

Biologia plantarum 1980, 22:239-240 | DOI: 10.1007/BF02892750

Original Papers

Influence of physiologically active substances of the soil humus on the activity of glucose-6-phosphate-dehydrogenase in pea (Pisum sativum L.) roots

F. PospíšiL

Biologia plantarum 1980, 22:161 | DOI: 10.1007/BF02892733

The influence of phenolic and humic acids on the activity of glucose-6-phosphate-dehydrogenase in the roots of pea under aseptic conditions has been investigated. It seems clear that vanillic and protocatechuic acids inhibit the enzyme activity in the excised roots of pea, but their dry weight increases in relation to the control. Gallic acid stimulates the G-6-PD activity in the roots of whole plants. The humic acids influence neither the enzyme activity nor the dry weight of pea seedlings after short-term treatment.

Effect of humic acids on the inhibition of pea choline esterase and choline acyltransferase with malathion

Rita Malini de Almeida, F. Pospíšil, Květa Vacková, M. Kutáček

Biologia plantarum 1980, 22:167 | DOI: 10.1007/BF02892734

Humic acids in a concentration of up to 100 μg ml-1 affect the activity of choline esterase of pea only weakly during sinapin (choline ester of sinapic acid) hydrolysis. The inhibition is stronger at higher concentrations. A similar course of inhibition with humic acids was also observed during the synthesis of sinapin catalysed with choline acyltransferase present in the same enzyme extract. The organophosphate malathion is a strong inhibitor of both the hydrolysis and the synthesis of sinapin. Thus, for example, at a 3.5 × 10-5 M concentration it causes 85% inhibition of pea choline esterase. However, in combination with...

Nitrate reductase activity in the course of cucumber leaf ontogenesis

Marie Bláhová, V. Segeťa

Biologia plantarum 1980, 22:176 | DOI: 10.1007/BF02892735

Nitrate reductase activity in the leaves of young plants of cucumber.Cucumis sativus L. cv. Bílská, as determined bothin vitro andin vivo and expressed in terms of fresh weight, gradually changes in dependence on the ontogenetic development of the plants, reaching its maximum before full expansion of the leaves.

The content and composition of the essential oil in the course of Anthodium development in wild camomile (Matricaria chamomilla L.)

M. Repčák, Jolana Halásová, R. Hončariv, D. Podhradský

Biologia plantarum 1980, 22:183 | DOI: 10.1007/BF02892736

A study was made of the changes in the content of essential oil and its components (farnesene, (-)-α-bisabolol, (-)-α-bisabololoxide A, (-)-α-bisabololoxide B and spathulenol, α-bisabolonoxide A, chamazulene, cis en-in-dicycloether) and of the number of secretory canals in the receptacles during the beginning of flowering, full flowering and the termination of flowering of camomile anthodia. The total content of the essential oil was highest during the beginning of flowering and lowest during its termination. The proportion of the individual sesquiterpenes in the essential oil underwent changes mostly during the beginning and...

Isolation of mesophyll cells fromValerianella olitoria Poll. and evaluation of their ability for 3-O-methyl-D-glucose absorption

Françoise de Bock

Biologia plantarum 1980, 22:192 | DOI: 10.1007/BF02892738

This paper describes a procedure for isolation and preservation of mesophyll cells, adapted toValerianella olitoria. We have tested the best conditions of temperature, pH, enzyme concentration, plasmolysis and the action of several adjuvants, dithioerythritol and mineral nutrients. The method described gives a high yield of viable cells. The ability of isolated cells and cellsin situ to take up methyl-glucose is compared.

Protection of potato virus X infection by plant extracts

L. P. Awasthi, K. Mukerjee

Biologia plantarum 1980, 22:205 | DOI: 10.1007/BF02892740

Extracts from the roots ofBoerhaavia diffusa L., stems ofCuscuta reflexa Roxb. or leaves ofEuphorbia hirta L. have shown a potential protective effect on the infection of potato virus X, in hypersensitive and systemic hosts. The inhibition by these extracts was systemic and sensitive to actinomycin D.

The localization and the isoenzymes of α- and β-Galactosidases in root tips

K. Beneš, Věra Hadačová

Biologia plantarum 1980, 22:210 | DOI: 10.1007/BF02892742

The localization was studied of α- and β-galactosidases in frozen sections of Ca-formol fixed root tips using simultaneous azocoupling reaction. In all species studied (Allium cepa,Cucurbita maxima, Lupinus albus, Pisum sativum, Vicia faba, Zea mays) positive results were obtained, the localization being ubiquitous (according to localization typology given here).
InVicia faba andZea mays the isoenzymes of α- and β-galactosidases were revealed by means of acrylamide gel electrophoresis, using authors' modification of Reisfeld method, in whole root tips, particular growth zones and separately in cortex...

Cytokinin-like activity in sea water andFucus vesiculosus L

Teresa Kentzer, R. Synak, Krystyna Burkiewtcz, A. Banas

Biologia plantarum 1980, 22:218 | DOI: 10.1007/BF02892743

Cytokinin activity was demonstrated in the Baltic sea waters. This activity was always higher in the near-bottom water, taken from theFucus vesiculosuszone, than in the superficial zone. A cytokinin-like substance showing properties typical of 6-(3-methyl-2-butenylamino)purine (2iP) was also present in extracts from the thallus ofFucus. It was found that exogenously applied cytokinins, in some concentration ranges, markedly increased the number of adventitious branches formed on the cut surface of the thallus fragments. The growth responses of the investigated plant tissues to the different cytokinins varied according to season and kind...

Stimulation of bulb growth in onion (Allium cepa L.) by N,N-diethyl-N-(2-hydroxyethyl)glycine

J. S. Knypl

Biologia plantarum 1980, 22:226-230 | DOI: 10.1007/BF02892745

Both N,N-diethyl-N-(2-hydroxyethyl)glycine (DEHEG) and N,N-diethyl-2-oxomorpholinium chloride (DEOMC) in an optimal dosage of 4 × l0 μmoles per seedling initially accelerated then retarded leaf growth in onion, and doubled the yield of bulbs in field conditions. Gibberellin and auxin nullified the latter effect. It is concluded that DEHEG represents a physiologically active form of DEOMC.

Root formation in pea cuttings: Effects of combined application of auxin and cytokinin at different developmental stages

S. Mohammed

Biologia plantarum 1980, 22:231 | DOI: 10.1007/BF02892747

Cuttings of pea cv. Alaska and ov. Kelwo were both decapitated and disbudded at different time intervals after cutting. Auxin and cytokinin combined in different ratios were applied to the upper part of the decapitated and disbudded cuttings. The effects of different ratios of auxin and cytokinin were not the same when applied at different developmental stages of the root initiation phase. The results seem to demonstrate an interaction between auxin and cytokinin at different ratios throughout the root initiation phase. The effects of combined application of auxin and cytokinin suggest that different stages of the root initiation phase require different...

Book Review

Pflanzenanatomisches praktikum I

Ingrid Tichá

Biologia plantarum 1980, 22:204 | DOI: 10.1007/BF02892739

Ecology and phytogeography of high altitude plants of the Northwest Himalaya

Danuše Hodáňová

Biologia plantarum 1980, 22:209 | DOI: 10.1007/BF02892741

Perspectives in grassland ecology

Danuše Hodáňová

Biologia plantarum 1980, 22:225 | DOI: 10.1007/BF02892744

Tropical trees and forests. An architectural analysis

Jiřina Slavíková

Biologia plantarum 1980, 22:230 | DOI: 10.1007/BF02892746

Vegetation and production ecology of an Alaskan Arctic Tundra

Danuše Hodáňová

Biologia plantarum 1980, 22:236 | DOI: 10.1007/BF02892748

Brief Communications

A leaf dip method for routine identification of plant viruses using the latex agglutination test

J. Polák

Biologia plantarum 1980, 22:237-238 | DOI: 10.1007/BF02892749

A description is presented of a simplified procedure for the serological diagnosis of plant viruses which utilizes the high sensitivity of the latex test. Small pieces of the plant examined are soaked in a drop of water, buffer, or directly in the sensibilized antiserum. Following the reaction with the antiserum the virus is evidenced by visual evaluation of the presence of agglutination. The procedure is applicable for routine serological diagnosis; it demonstrates even small virus concentrations and is as reliable as the normal procedure of the latex test. Using this simplified procedure the following viruses were demonstrated in various host plants:...