Biologia plantarum 58:427-435, 2014 | DOI: 10.1007/s10535-014-0420-y

Identification and functional analysis of BABY BOOM genes from Rosa canina

H. F. Yang1, Y. P. Kou1, B. Gao1, T. M. A. Soliman1, K. D. Xu2, N. Ma1, X. Cao3, L. J. Zhao1,*
1 Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing, P.R. China
2 Department of Life Science, Zhoukou Normal University, Henan, P.R. China
3 College of Agriculture, Liaocheng Uuniversity, Shandong, P.R. China

BABY BOOM (BBM), initially identified in Brassica napus, can enhance the shoot regeneration capacity in tissue culture and is involved in the conversion from the vegetative to embryogenic state. This study aimed to isolate BBM orthologue genes from Rosa canina and analyse their functions. Two full-length cDNAs, designated RcBBM1 and RcBBM2, were isolated from R. canina by the rapid amplification of cDNA ends (RACE). The predicted amino acid sequences of the two RcBBMs contained the bbm-1 motif and the motifs typically conserved in the eudicotANT (euANT) lineage. Phylogenetic tree analysis showed that the RcBBMs were most closely related to the BBM orthologue genes identified in Glycine max and Medicago truncatula. The transcripts of the RcBBMs were detected in young roots, calluses, and protocorm-like bodies (PLBs), whereas they were undetectable in stems, leaves, and flowers. RcBBM1-GFP and RcBBM2-GFP fusion proteins were both localized in the nucleus. 35S::RcBBM1 and 35S::RcBBM2 transgenic Arabidopsis thaliana lines exhibited enhanced shoot regeneration capacity in tissue culture, but did not undergo spontaneous somatic embryogenesis. The results suggest that RcBBMs may be candidate genes for improving the shoot regeneration efficiency of R. canina.

Keywords: in vitro culture; RACE; RcBBM gene; rose; shoot regeneration; somatic embryogenesis
Subjects: BABY BOOM gene; in vitro culture; RACE; shoot regeneration; somatic embryogenesis; amino acid sequences; phylogenetic tree; subcellular localization

Received: May 22, 2013; Revised: January 11, 2014; Accepted: January 13, 2014; Published: September 1, 2014Show citation

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Yang, H.F., Kou, Y.P., Gao, B., Soliman, T.M.A., Xu, K.D., Ma, N., Cao, X., & Zhao, L.J. (2014). Identification and functional analysis of BABY BOOM genes from Rosa canina. Biologia plantarum, 58(3), 427-435. doi: 10.1007/s10535-014-0420-y.
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    References

    1. Aoyama, T., Hiwatashi, Y., Shigyo, M., Kofuji, R., Kubo, M., Ito, M., Hasebe, M.: AP2-type transcription factors determine stem cell identity in the moss Physcomitrella patens. - Development 139: 3120-3129, 2012. Go to original source...
    2. Bendahmane, M., Dubois, A., Raymond, O., Bris, M.L.: Genetics and genomics of flower initiation and development in roses. - J. exp. Bot. 64: 847-857, 2013. Go to original source...
    3. Bi, L., Liu, F.L., Dong, A.X., Zhou, X.Y., Ma, N., Zhao, L.J.: Establishment of genetic transformation system in protcorm like-body (PLB) of Rosa canina. - J. Nucl. agr. Sci. 26: 82-86, 2011.
    4. Boutilier, K., Offringa, R., Sharma, V.K., Kieft, H., Ouellet, T., Zhang, L., Hattori, J., Liu, C.M., Van Lammeren, A.A.M., Miki, B.L.A., Custers, J.B.M., Van Lookeren Campagne, M.M.: Ectopic expression of BABY BOOM triggers a conversion from vegetative to embryonic growth. - Plant Cell 14: 1737-1749, 2002. Go to original source...
    5. Bremer, B., Bremer, K., Chase, M.W., Fay, M.F., Reveal, J.L., Soltis, D.E., Soltis, P.S., Stevens, P.F.: An update of the angiosperm phylogeny group classification for the orders and families of flowering plants: APG III. - Bot. J. Linn. Soc. 161: 105-121, 2009.
    6. Candela, M., Velázquez, I., Cruz, B.D.L., Sendino, A.M., Pena, A.D.L.: Differences in in vitro plant regeneration ability among four Arabidopsis thaliana ecotypes. - In Vitro cell. dev. Biol. Plant 37: 638-643, 2001. Go to original source...
    7. Chugh, A., Khurana, P.: Gene expression during somatic embryogenesis recent advances. - Curr. Sci. 83: 715-730, 2002.
    8. Clough, S.J., Bent, A.F.: Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. - Plant J. 16: 735-743, 1998. Go to original source...
    9. Condliffe, P.C., Davey, M.R., Power, J.B., Koehorst-Van Putten, H., Visser, P.B.: An optimized protocol for rose transformation applicable to different cultivars. - Acta Hort. 612: 115-120, 2003. Go to original source...
    10. Feng, J.X., Liu, D., Pan, Y., Gong, W., Ma, L.G., Luo, J.C., Deng, X.W., Zhu, Y.X.: An annotation update via cDNA sequence analysis and comprehensive profiling of developmental, hormonal or environmental responsiveness of the Arabidopsis AP2/EREBP transcription factor gene family. - Plant mol. Biol. 59: 853-868, 2005. Go to original source...
    11. Floyd, S.K., Bowman, J.L.: The ancestral developmental tool kit of land plants. - Int. J. Plant Sci. 168: 1-35, 2007. Go to original source...
    12. Jiang, F.X., Liu, F.L., Zhao, L.J.: Overexpression of RaWUS gene in Rosa canina induce formation of adventitious bud from root tip of transgenic tobacco. - Sci. silvae sin. 11: 43-52, 2011.
    13. Jiang, F.X., Tian, C.W., Zhang, J.F., Ma, N., Zhang, C.Q., Zhao, L.J.: Microscopic observations on development of protocorm-like body of Rosa canina L. - Plant Physiol. Commun. 46: 589-596, 2010.
    14. Khaitová, L., Werlemark, G., Nybom, H., Kovařík, A.: Frequent silencing of rDNA loci on the univalent-forming genomes contrasts with their stable expression on the bivalentforming genomes in polyploid dogroses (Rosa sect. Caninae). - Heredity 104: 113-120, 2010. Go to original source...
    15. Kim, S.: Phylogeny and domain evolution in the APETALA2-like gene family. - Mol. Biol. Evol. 23: 107-120, 2005. Go to original source...
    16. Kulinska-Lukaszek, K., Tobojka, M., Adamiok, A., Kurczynska, E.U.: Expression of the BBM gene during somatic embryogenesis of Arabidopsis thaliana. - Biol. Plant. 56: 389-394, 2012. Go to original source...
    17. Li, J., Ruan, Y., Liu, C., Deng, H., Fan, L.: Progress in tissue culture and genetic transformation of roses. - Acta bot. boreal. occident. sin. 27: 1479-1483, 2007.
    18. Marchant, R., Power, J.B., Lucas, J.A., Davey, M.R.: Biolistic transformation of rose (Rosa hybrida L.) - Ann. Bot. 81: 109-114, 1998. Go to original source...
    19. Mishra, S., Sanyal, I., Amla, D.V.: Changes in protein pattern during different developmental stages of somatic embryos in chickpea. - Biol. Plant. 56: 613-619, 2012. Go to original source...
    20. Nole-Wilson, S., Tranby, T.L., Krizek, B.A.: AINTEGUMENTA-like (AIL) genes are expressed in young tissues and may specify meristematic or division-competent states. - Plant mol. Biol. 57: 613-628, 2005. Go to original source...
    21. Ouakfaoui, S.E., Schnell, J., Abdeen, A., Colville, A., Labbé, H., Han, S., Baum, B., Laberge, S., Miki, B.: Control of somatic embryogenesis and embryo development by AP2 transcription factors. - Plant mol. Biol. 74: 313-326, 2010. Go to original source...
    22. Pata, M.O., Wu, B.X., Bielawski, J., Xiong, T.C., Hannun, Y.A., Ng, C.K.Y.: Molecular cloning and characterization of OsCDase, a ceramidase enzyme from rice. - Plant J. 55: 1000-1009, 2008. Go to original source...
    23. Qiao, M., Zhao, Z.J., Xiang, F.N.: Arabidopsis thaliana in vitro shoot regeneration is impaired by silencing of TIR1. - Biol. Plant. 56: 409-414, 2012. Go to original source...
    24. Ray, A., Ray, S., Mukhopadhyay, S., Ray, M.: Methylglyoxal with glycine or succinate enhances differentiation and shoot morphogenesis in Nicotiana tabacum callus. - Biol. Plant. 57: 219-223, 2013. Go to original source...
    25. Riechmann, J., Meyerowitz, E.: The AP2/EREBP family of plant transcription factors - Biol. Chem. 379: 633-646, 1998.
    26. Ritz, C.M., Schmuths, H., Wissemann, V.: Evolution by reticulation: European dogroses originated by multiple hybridization across the genus Rosa. - J. Hered. 96: 4-14, 2004. Go to original source...
    27. Sala, K., Potocka, I., Kurczynska, E.: Spatio-temporal distribution and methyl-esterification of pectic epitopes provide evidence of developmental regulation of pectins during somatic embryogenesis in Arabidopsis thaliana. - Biol. Plant. 57: 410-416, 2013. Go to original source...
    28. Skoog, F., Miller, C.O.: Chemical regulation of growth and organ formation in plant tissues cultured in vitro. - Symp. Soc. exp. Biol. 11: 118-131, 1957.
    29. Srinivasan, C., Liu, Z., Heidmann, I., Supena, E.D.J., Fukuoka, H., Joosen, R., Lambalk, J., Angenent, G., Scorza, R., Custers, J.B.M., Boutilier, K.: Heterologous expression of the BABY BOOM AP2/ERF transcription factor enhances the regeneration capacity of tobacco (Nicotiana tabacum L.). - Planta 225: 341-351, 2007.
    30. Tanaka, Y., Katsumoto, Y., Brugliera, F., Mason, J.: Genetic engineering in floriculture. - Plant Cell Tissue Organ Cult. 80: 1-24, 2005. Go to original source...
    31. Tian, C.W., Chen, Y., Zhao, X.L., Zhao, L.J.: Plant regeneration through protocorm-like bodies induced from rhizoids using leaf explants of Rosa spp. - Plant Cell Rep. 27: 823-831, 2008. Go to original source...
    32. Tun, O.M., Lipsky, A., Luzzatto Knaan, T., Kerem, Z., Yedidia, I.: The plant activator BTH promotes Ornithogalum dubium and O. thyrsoides differentiation and regeneration in vitro. - Biol. Plant. 57: 41-48, 2013. Go to original source...
    33. Vergne, P., Maene, M., Gabant, G., Chauvet, A., Debener, T., Bendahmane, M.: Somatic embryogenesis and transformation of the diploid Rosa chinensis cv. Old Blush. - Plant Cell Tissue Organ Cult. 100: 73-81, 2010. Go to original source...
    34. Wang, J., Long, Y., Wu, B.D., Liu, J., Jiang, C.C., Shi, L., Zhao, J.W., King, G.J., Meng, J.L.: The evolution of Brassica napus FLOWERING LOCUST paralogues in the context of inverted chromosomal duplication blocks. - BMC Evol. Biol. 9: 271, 2009. Go to original source...
    35. Zakizadeh, H., Lütken, H., Sriskandarajah, S., Serek, M., Müller, R.: Transformation of miniature potted rose (Rosa hybrida cv. Linda) with PSAG12-ipt gene delays leaf senescence and enhances resistance to exogenous ethylene. - Plant Cell Rep. 32: 195-205, 2013. Go to original source...
    36. Zeng, S., Liang, S., Zhang, Y.Y., Wu, K.L., Teixeira da Silva, J.A., Duan, J.: In vitro flowering red miniature rose. - Biol. Plant. 57: 401-409, 2013. Go to original source...
    37. Zhang, J.H., Zhang, S.G., Wu, T., Han, S.Y., Yang, W.H., Qi, L.W.: Expression analysis of five miRNAs and their precursors during somatic embryogenesis in Larch. - Chin. Bull. Bot. 47: 462-473, 2012.

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