Biologia plantarum 2014, 58:515-523 | DOI: 10.1007/s10535-014-0414-9

Identification of heat responsive genes in cotton

U. Demirel1,*, A. Gür2, N. Can3, A. R. Memon4
1 Department of Agricultural Genetic Engineering, Nigde University, Nigde, Turkey
2 Department of Field Crops, Harran University, Sanliurfa, Turkey
3 Department of Biology, Harran University, Sanliurfa, Turkey
4 Department of Genetics and Bioengineering, International University of Sarajevo, Sarajevo, Bosnia and Herzegovina

High summer temperature negatively affects cotton yield, and better understanding of genetic mechanisms of heat stress responses in cotton may facilitate development of new heat tolerant cultivars. We attempted to determine heat responsive genes in cotton using tolerant (Stoneville 453, BA 119) and susceptible (Nazilli 84S) cultivars. Twenty five expressed sequence tags (ESTs) were sequenced and studied for gene homology. Sixteen ESTs were significantly similar to known genes, whereas eight ESTs were similar to not annotated cDNA clones and 1 EST did not show homology to any known gene. Expression of some ESTs was analyzed by quantitative real-time PCR and IAA-ala hydrolase (IAR3), folylpolyglutamate synthase (FPGS3), and two not annotated ESTs (GhHS126 and GhHS128) were consistently up-regulated under both short- and long-term heat stress. Since cotton is considered relatively more heat tolerant than most of the other crops, it can be suggested that these genes and ESTs could play a significant role in heat tolerance. In addition, GhHS126 and GhHS128 might be parts of the new candidate genes for heat tolerance.

Keywords: differentially expressed genes; Gossypium hirsutum; high temperature; real-time quantitative PCR
Subjects: heat responsive genes; temperature - high; heat tolerance; gene expression; cotton
Species: Gossypium hirsutum

Received: September 1, 2013; Revised: January 7, 2014; Accepted: January 21, 2014; Published: September 1, 2014Show citation

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Demirel, U., Gür, A., Can, N., & Memon, A.R. (2014). Identification of heat responsive genes in cotton. Biologia plantarum, 58(3), 515-523. doi: 10.1007/s10535-014-0414-9.
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    References

    1. Altschul, S.F., Madden, T.L., Schäffer, A.A., Zhang, J., Zhang, Z., Miller, W., Lipman, D.J.: Gapped BLAST and PSIBLAST: a new generation of protein database search programs. - Nucl. Acids Res. 25: 3389-3402, 1997. Go to original source...
    2. Artico, S., Nardeli, S., Brilhante, O., Grossi-de-Sa, M., Alves-Ferreira, M.: Identification and evaluation of new reference genes in Gossypium hirsutum for accurate normalization of real-time quantitative RT-PCR data. - BMC Plant Biol. 10: 49, 2010. Go to original source...
    3. Ashraf, M., Saeed, M.M., Qureshii, M.J.: Tolerance to high temperature in cotton (Gossypium hirsutum L.) at initial growth stages. - Environ. exp. Bot. 34: 275-283, 1994. Go to original source...
    4. Bednarz, C.W., Van Iersal, M.W.: Temperature response of whole-plant CO2 exchange rate of four upland cotton cultivars differing in leaf shape and leaf pubescence. - Commun. Soil Sci. Plant Anal. 32: 2485-2501, 2001. Go to original source...
    5. Catala, R., Ouyang, J., Abreu, I.A., Hu, Y., Seo, H., Zhang, X., Chua, N.H.: The Arabidopsis E3 SUMO ligase SIZ1 regulates plant growth and drought responses. - Plant Cell 19: 2952-2966, 2007. Go to original source...
    6. Cheong, Y.H., Pandey, G.K., Grant, J.J., Batistic, O., Li, L., Kim, B.G., Lee, S.C., Kudla, J., Luan, S.: Two calcineurin b-like calcium sensors, interacting with protein kinase CIPK23, regulate leaf transpiration and root potassium uptake in Arabidopsis. - Plant J. 52: 223-239, 2007. Go to original source...
    7. Çopur, O., Birgul, I.H., Haliloglu, H., Beyyavas, V.: The effect of different harvesting times on seed-set efficiency in cotton (Gossypium hirsutum L.) varieties. - Afr. J. Biotechnol. 9: 6654-6659, 2010.
    8. Dunkley, T.P.J., Hester, S., Shadforth, I.P., Runions, J., Weimar, T., Hanton, S.L., Griffin, J.L., Bessant, C., Brandizzi, F., Hawes, C., Watson, R.B., Dupree, P., Lilley, K.S.: Mapping the Arabidopsis organelle proteome. - Proc. nat. Acad. Sci. USA 103: 6518-6523, 2006. Go to original source...
    9. Escalettes, V.S.L., Hullot, C., Wawrzyńczak, D., Mathieu, E., Eyquard, J.P., Gall, O.L., Decroocq, V.: Plum pox virus induces differential gene expression in the partially resistant stone fruit tree Prunus armeniaca cv. Goldrich. - Gene 374: 96-103, 2006. Go to original source...
    10. Essemine, J., Govindachary, S., Ammar, S., Bouzid, S., Carpentier, R.: Functional aspects of the photosynthetic light reactions in heat stressed Arabidopsis deficient in digalactosyl-diacylglycerol. - J. Plant Physiol. 168: 1526-1533, 2011. Go to original source...
    11. Ginzberg, I., Barel, G., Ophir, R., Tzin, E., Tanami, Z., Muddarangappa, T., De Jong, W., Fogelman, E.: Transcriptomic profiling of heat-stress response in potato periderm. - J. exp. Bot. 60: 4411-4421, 2009. Go to original source...
    12. Gong, D., Gong, Z., Guo, Y., Zhu, J.K.: Expression, activation, and biochemical properties of a novel Arabidopsis protein kinase. - Plant Physiol. 129: 225-234, 2002. Go to original source...
    13. Gong, H., Chen, G., Li, F., Wang, X., Hu, Y., Bi, Y.: Involvement of G6PDH in heat stress tolerance in the calli from Przewalskia tangutica and Nicotiana tabacum. - Biol. Plant. 56: 422-430, 2012. Go to original source...
    14. Gür, A., Demirel, U., Özden, M., Kahraman, A., Çopur, O.: Diurnal gradual heat stress affects antioxidant enzymes, proline accumulation and some physiological components in cotton (Gossypium hirsutum L.). - Afr. J. Biotechnol. 9: 1008-1015, 2010.
    15. Halford, N.G., Hey, S., Jhurreea, D., Laurie, S., McKibbin, R.S., Paul, M., Zhang, Y.: Metabolic signaling and carbon partitioning: role of Snf1-related (SnRK1) protein kinase. - J. exp. Bot. 54: 467-475, 2003. Go to original source...
    16. Hruz, T., Laule, O., Szabo, G., Wessendorp, F., Bleuler, S., Oertle, L., Widmayer, P., Gruissem, W., Zimmermann, P.: Genevestigator V3: a reference expression database for the meta-analysis of transcriptomes. - Advances in Bioinformatics 420747, 2008.
    17. Kim, K.N., Cheong, Y.H., Grant, J.J., Pandey, G.K., Luan, S.: CIPK3, a calcium sensor-associated protein kinase that regulates abscisic acid and cold signal transduction in Arabidopsis. - Plant Cell 15: 411-423, 2003. Go to original source...
    18. Kinoshita, N., Wang, H., Kasahara, H., Liu, J., MacPherson, C., Machida, Y., Kamiya, Y., Hannah, M.A., Chua, N.: IAAAla Resistant3, an evolutionarily conserved target of miR167, mediates Arabidopsis root architecture changes during high osmotic stress. - Plant Cell 24: 3590-3602, 2012. Go to original source...
    19. Kolukisaoglu, Ü., Weinl, S., Blazevic, D., Batistic, O., Kulda, J.: Calcium sensors and their interacting protein kinases: genomics of the Arabidopsis and rice CBL-CIPK signaling networks. - Plant Physiol. 134: 43-58, 2004. Go to original source...
    20. Kwon, Y., Kim, S.H., Jung, M.S., Kim, M.S., Oh, J.E., Ju, H,W., Kim, K.I., Vierling, E., Lee, H., Hong, S.W.: Arabidopsis hot2 encodes an endochitinase-like protein that is essential for tolerance to heat, salt and drought stresses. - Plant J. 49: 184-193, 2007. Go to original source...
    21. Liang, P., Pardee, A.B.: Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. - Science 257: 967-971, 1992. Go to original source...
    22. Liu, X., Baird, W.V.: Differential expression of genes regulated in response to drought or salinity stress in sunflower. - Crop Sci. 43: 678-687, 2003.
    23. Livak, K.J., Schmittgen, T.D.: Analysis of relative gene expression data using realtime quantitative PCR and the 2-ΔΔCt method. - Methods 25: 402-408, 2001. Go to original source...
    24. Mehrshahi, P., Gonzalez-Jorge, S., Akhtar, T.A., Ward, J.L., Santoyo-Castelazo, A., Marcus, S.E., Lara-Núñez, A., Ravanel, S., Hawkins, N.D., Beale, M.H., Barrett, D.A., Knox, J.P., Gregory III, J.F., Hanson, A.D., Bennett, M.J., DellaPenna, D.: Functional analysis of folate polyglutamylation and its essential role in plant metabolism and development. - Plant J. 64: 267-279, 2010. Go to original source...
    25. Oosterhuis, D.M.: Day or night high temperatures: a major cause of yield variability. - Cotton Grower 46: 8-9, 2002.
    26. Pandey, G.K., Cheong, Y.H., Kim, B.G., Grant, J.J., Li, L., Luan, S.: CIPK9: a calcium sensor-interacting protein kinase required for low-potassium tolerance in Arabidopsis. - Cell Res. 17: 411-421, 2007. Go to original source...
    27. Park, W., Scheffler, B.E., Bauer, P.J., Campbell, B.T.: Genomewide identification of differentially expressed genes under water deficit stress in upland cotton (Gossypium hirsutum L.). - BMC Plant Biol. 12: 90, 2012. Go to original source...
    28. Rahman, H., Malik, S.A., Saleem, M.: Heat tolerance of upland cotton during the fruiting stage evaluated using cellular membrane thermo stability. - Field Crops Res. 85: 149-158, 2004. Go to original source...
    29. Ravanel, S., Cherest, H., Jabrin, S., Grunwald, D., Surdin-Kerjan, Y., Douce, R., Rébeillé, F.: Tetrahydrofolate biosynthesis in plants: molecular and functional characterrization of dihydrofolate synthetase and three isoforms of folylpolyglutamate synthetase in Arabidopsis thaliana. - Proc. nat. Acad. Sci. USA 98: 15360-15365, 2001. Go to original source...
    30. Reddy, K.R., Kakani, V.G., Zhao, D., Koti, S., Gao, W.: Interactive effects of ultraviolet - B radiation and temperature on cotton growth, development, physiology and hyperspectral reflectance. - Photochem. Photobiol. 79: 416-427, 2004. Go to original source...
    31. Sánchez-Fernández, R., Davies, T.G.E., Coleman, J.O.D., Rea, P.A.: The Arabidopsis thaliana ABC protein superfamily, a complete inventory. - J. biol. Chem. 276: 30231-30244, 2001. Go to original source...
    32. Seki, M., Narusaka, M., Abe, H., Kasuga, M., Yamaguchi-Shinozaki, K., Carnici, P.Y., Hayashizaki, Y., Shinozaki, K.: Monitoring the expression pattern of 1300 Arabidopsis genes under drought and cold stresses by using a full length cDNA microarray. - Plant Cell 13: 61-72, 2001. Go to original source...
    33. Singh, K.B., Foley, R.C., Onate-Sanchez, L.: Transcription factors in plant defense and stress responses. - Curr. Opin. Plant Biol. 5: 430-436, 2002. Go to original source...
    34. Singh, R.P., Vara Prasad, P.V., Sunita, K., Giri, S.N., Reddy, K.R.: Influence of high temperature and breeding for heat tolerance in cotton: a review. - Adv. Agron. 93: 313-386, 2007. Go to original source...
    35. Sotirios, K.A., Argyrokastritis, A., Loukas, M., Eliopoulos, E., Tsakas, S., Kaltsikes, P.J.: Isolation and characterization of stress related heat shock protein calmodulin binding gene from cultivated cotton (Gossypium hirsutum L.). - Euphytica 147: 343-351, 2006. Go to original source...
    36. Srivastava, A.C., Ramos-Parra, P.A., Bedair, M., Robledo-Hernández, A.L., Tang, Y., Sumner, L.W., Díaz de la Garza, R.I., Blancaflor, E.B.: The folylpolyglutamate synthetase plastidial isoform is required for postembryonic root development in Arabidopsis. - Plant Physiol. 155: 1237-1251, 2011. Go to original source...
    37. Tao, P., Liu, L., Wang, J.B.: Characterization of eight cytosolic sHSP genes and their expression in Capsella bursa-pastoris. - Biol. Plant. 56: 648-656, 2012. Go to original source...
    38. Titarenko, E., Rojo, E., Leon, J., Sanchez-Serrano, J.J.: Jasmonic acid-dependent and -independent signaling pathways control wound-induced gene activation in Arabidopsis thaliana. - Plant Physiol 115: 817-826, 1997. Go to original source...
    39. Ueda, A., Shi, W., Nakamura, T., Takabe, T.: Analysis of saltinducible genes in barley roots by differential display. - J. Plant Res. 115: 119-130, 2002. Go to original source...
    40. Vierling, E.: The role of heat shock proteins in plants. - Annu. Rev. Plant Physiol. Plant mol. Biol. 42: 579-620, 1991. Go to original source...
    41. Wang, P., Duan, W., Takabayashi, A., Endo, T., Shikanai, T., Ye, J.Y., Mi, H.: Chloroplastic NAD(P)H dehydrogenase in tobacco leaves functions in alleviation of oxidative damage caused by temperature stress. - Plant Physiol. 141: 465-474, 2006. Go to original source...
    42. Weinl, S., Kudla, J.: The CBL-CIPK Ca2+-decoding signaling network: function and perspectives. - New Phytol. 184: 517-528, 2009. Go to original source...
    43. Xu, P., Liu, Z., Fan, X., Gao, J., Zhang, X., Zhang, X., Shen X.: De novo transcriptome sequencing and comparative analysis of differentially expressed genes in Gossypium aridum under salt stress. - Gene 525: 26-34, 2013. Go to original source...
    44. Yamaguchi-Shinozaki, K., Kasuga, M., Liu, Q., Nakashima, K., Sakuma, Y., Abe, H., Shinwari, Z.K., Seki, M., Shinozaki, K.: Biological mechanisms of drought stress response. - JIRCAS Working Rep. 23: 1-8, 2002.
    45. Zhang, X., Rerksiri, W., Liu, A., Zhou, X., Xiong, H., Xiang, J., Chen, X., Xiong, X.: Transcriptome profile reveals heat response mechanism at molecular and metabolic levels in rice flag leaf. - Gene 530: 185-192, 2013a. Go to original source...
    46. Zhang, X., Yao, D., Wang, Q., Xu, W., Wei, Q., Wang, C., Liu, C., Zhang, C., Yan, H., Ling, Y., Su, Z., Li, F.: mRNA-seq analysis of the Gossypium arboretum transcriptome reveals tissue selective signaling in response to water stress during seedling stage. - PLoS ONE 8: e54762, 2013b. Go to original source...
    47. Zhang, Y., Mian, M.A.R., Chekhovskiy, K., So, S., Kupfer, D., Lai, H., Roe, B.A.: Differential gene expression in Festuca under heat stress conditions. - J. exp. Bot. 56: 897-907, 2005. Go to original source...

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