Biologia plantarum 57:189-192, 2013 | DOI: 10.1007/s10535-012-0261-5

Dark preincubation improves shoot organogenesis from Rhodiola crenulata leaf explants

Y. Zhao1,3, A. R. Stiles1, P. K. Saxena2, C. Z. Liu1,*
1 Institute of Process Engineering, Chinese Academy of Sciences, Beijing, P.R. China
2 Department of Plant Agriculture, University of Guelph, Guelph, Canada
3 Graduate School of the Chinese Academy of Sciences, Beijing, P.R. China

An efficient in vitro plant regeneration system has been developed using dark preincubated leaf explants of Rhodiola crenulata, a traditional Tibetan medicinal plant. The leaf explants, preincubated in the dark for 5 d, developed an average of 9.1 shoots per explant on a medium containing 15 μM N 6-benzyladenine (BA) and 2.5 μM gibberellic acid (GA3). The biochemical mechanism underlying dark-induced shoot organogenesis was investigated by measuring polyphenol oxidase (PPO) activity. Dark preincubation significantly reduced PPO activity in leaf explants during the initial period of shoot organogenesis and reduced browning compared to explants cultured in the light. Up to 88.4 % of the regenerated shoots formed roots and developed into complete plantlets on a medium containing 5 μM indoleacetic acid (IAA) within 25 d. Approximately 82 % of the regenerated plantlets survived transplantation and grew vigorously in the greenhouse.

Keywords: auxin; cytokinin; gibberellic acid; polyphenol oxidase
Subjects: in vitro culture; dark preincubation; auxin; cytokinin; gibberellic acid; polyphenol oxidase
Species: Rhodiola crenulata

Received: September 29, 2011; Accepted: April 17, 2012; Published: March 1, 2013Show citation

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Zhao, Y., Stiles, A.R., Saxena, P.K., & Liu, C.Z. (2013). Dark preincubation improves shoot organogenesis from Rhodiola crenulata leaf explants. Biologia plantarum, 57(1), 189-192. doi: 10.1007/s10535-012-0261-5.
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