Biologia Plantarum 63: 183-192, 2019 | DOI: 10.32615/bp.2019.021

Overexpression of CBL interacting protein kinase 2 improves plant tolerance to salinity and mercury

W.H. Pan1, Z.Z. Zheng2, X. Yan2,3, J.Q. Shen2, J.X. Shou1, L.X. Jiang4, J.W. Pan3,*
1 College of Life Sciences, Shaoxing University, Shaoxing, Zhejiang 312000, P.R. China
2 College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, Zhejiang 321004, P.R. China
3 School of Life Sciences, Lanzhou University, Lanzhou 730000, P.R. China
4 College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, P.R. China

In plants, calcineurin B-like proteins (CBLs) and CBL-interacting protein kinases (CIPKs) regulate Ca2+ signalling and so responses to biotic and abiotic stresses. However, the details of specific CIPKs functions in various stress responses are poorly understood. Here, we report roles of dicot and monocot CIPK2 genes in response to salinity and heavy metals. Arabidopsis thaliana AtCIPK2 was found to be universally expressed in different tissues and organs and furthermore induced by salinity. Overexpression of AtCIPK2 or Tibetan Plateau wild barley (Hordeum spontaneum) HsCIPK2 in Arabidopsis alleviated toxic effects of NaCl and mercury on seed germination and root growth. Similarly, reduced toxic effects of copper and cadmium on seed germination, but not on root growth, were observed in these transgenic lines. Live-cell fluorescence imaging analysis revealed that HsCIPK2 was predominantly distributed in the cytoplasm and nucleus and weakly localized at the plasma membrane (PM), but its PM association was rapidly enhanced upon exposure to high salinity and mercury. These results suggest an involvement of CIPK2 in plant tolerance to salinity and mercury and provide a new insight into physiological functions of CIPKs in plant response to heavy metals.

Keywords: cadmium, copper, gene expression, NaCl, root growth, seed germination, subcellular localization

Accepted: November 15, 2018; Prepublished online: November 15, 2018; Published online: January 19, 2019Show citation

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Pan, W.H., Zheng, Z.Z., Yan, X., Shen, J.Q., Shou, J.X., Jiang, L.X., & Pan, J.W. (2019). Overexpression of CBL interacting protein kinase 2 improves plant tolerance to salinity and mercury. Biologia plantarum, 63, 183-192. doi: 10.32615/bp.2019.021.
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