Biologia plantarum 2010, 54:502-508 | DOI: 10.1007/s10535-010-0088-x

Leaf structural modifications in Populus × euramericana subjected to Zn excess

D. Di Baccio1,*, A. Minnocci1, L. Sebastiani1
1 BioLabs, Scuola Superiore Sant'Anna, Pisa, Italy

In previous experiments elevated but sub-symptomatic applications of Zn (0.1 mM and 1 mM) caused impairments in growth parameters and photosynthetic performance of Populus × euramericana (Dode) Guinier clone I-214. The aim of this work was to evaluate leaf morphological and anatomical traits in this clone in response to the same Zn concentrations. The results showed that Zn treatments induced variations in leaf dry mass, area, mesophyll thickness, intercellular spaces, stomatal density and size. Stronger modifications, especially concerning stomata characteristics induced by 1 mM Zn, were consistent with physiological impairments while those induced by 0.1 mM Zn suggested a compensatory strategy for maintaining functional integrity.

Keywords: cryo-scanning electron microscopy; intercellular spaces; mesophyll thickness; poplar; stomatal density
Subjects: growth and growth analysis; leaf anatomy; leaf epidermis; leaf intracellulars; leaf mesophyll; poplar; Populus × euramericana; stomata number and size; zinc

Received: September 13, 2008; Accepted: June 12, 2009; Published: September 1, 2010Show citation

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Baccio, D., Minnocci, A., & Sebastiani, L. (2010). Leaf structural modifications in Populus × euramericana subjected to Zn excess. Biologia plantarum, 54(3), 502-508. doi: 10.1007/s10535-010-0088-x.
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    References

    1. Al Afas, N., Marron, N., Ceulemans, R.: Clonal variation in stomatal characteristics related to biomass production of 12 poplar (Populus) clones in a short rotation coppice culture. - Environ. exp. Bot. 58: 279-286, 2006. Go to original source...
    2. Broadley, M.R., White, P.J., Hammond, J.P., Zelko, I., Lux, A.: Zinc in plants. - New Phytol. 173: 677-702, 2007. Go to original source...
    3. Di Baccio, D., Castagna, A., Paoletti, E., Sebastiani, L., Ranieri, A.: Could the differences in O3 sensitivity between Eridano and I-214 poplar clones be related to different antioxidant defence and secondary metabolism induction in response to O3 influx? - Tree Physiol. 28: 1761-1772, 2008. Go to original source...
    4. Di Baccio, D., Kopriva, S., Sebastiani, L., Rennenberg, H.: Does glutathione metabolism have a role in the defence of poplar against zinc excess? - New Phytol. 167: 73-80, 2005. Go to original source...
    5. Di Baccio, D., Navari-Izzo, F., Izzo, R.: Seawater irrigation: antioxidant defence responses in leaves and roots of a sunflower (Helianthus annuus L.) ecotype. - J. Plant Physiol. 161: 1359-1366, 2004. Go to original source...
    6. Di Baccio, D., Tognetti, R., Minnocci, A., Sebastiani, L.: Responses of Populus × euramericana clone I-214 to zinc excess: carbon assimilation, structural modifications, metal distribution and cellular localization. - Environ. exp. Bot. 67: 153-163, 2009. Go to original source...
    7. Di Baccio, D., Tognetti, R., Sebastiani, L., Vitagliano C.: Responses of Populus deltoides × Populus nigra (Populus × euramericana) clone I-214 to high zinc concentrations. - New Phytol. 159: 443-452, 2003. Go to original source...
    8. Dickmann, D.I.: Photosynthesis and respiration by developing leaves of cottonwood (Populus deltoides Bartr.). - Bot. Gaz. 132: 253-259, 1971. Go to original source...
    9. Djingova, R., Wagner, G., Peshev, D.: Heavy metal distribution in Bulgaria using Populus nigra 'Italica' as a biomonitor. - Sci. Total Environ. 172: 151-158, 1995. Go to original source...
    10. Evans, J.R, Von Caemmerer, S., Setchell, B.A., Hudson, G.S.: The relationship between CO2 transfer conductance and leaf anatomy in transgenic tobacco with a reduce content of Rubisco. - Aust. J. Plant Physiol. 21: 475-495, 1994. Go to original source...
    11. Gatti, E.: Micropropagation of Ailanthus altissima and in vitro heavy metal tolerance. - Biol. Plant. 52: 146-148, 2008. Go to original source...
    12. Giachetti, G., Sebastiani, L.: Metal accumulation in poplar plant grown with industrial wastes. - Chemosphere 64: 446-454, 2006. Go to original source...
    13. Giachetti, G., Sebastiani, L.: Effects of tannery waste on growth dynamics and metal uptake in Salix alba L. - Plant Biosystems 141: 22-30, 2007. Go to original source...
    14. Gratani, L., Covone, F., Larcher, W.: Leaf plasticity in response to light of three evergreen species of the Mediterranean maquis. - Trees 20: 549-558, 2006. Go to original source...
    15. Gratani, L., Meneghini, M., Pesoli, P., Crescente, M.F.: Structural and functional plasticity of Quercus ilex seedlings of different provenances in Italy. - Trees 17: 515-521, 2003. Go to original source...
    16. Hermle, S., Vollenweider, P., Günthardt-Goerg, M.S., McQuattie, C.J., Matyssek, R.: Leaf responsiveness of Populus tremula and Salix viminalis to soil contaminated with heavy metals and acidic rainwater. - Tree Physiol. 27: 1517-1531, 2007. Go to original source...
    17. Laureysens, I., Blust, R., De Temmerman, L., Lemmens, C., Ceulemans, R.: Clonal variation in heavy metal accumulation and biomass production in a poplar coppice culture: I. Seasonal variation in leaf, wood and bark concentrations. - Environ. Pollut. 131: 485-494, 2004. Go to original source...
    18. Laureysens, I., De Temmerman, L., Hastir, T., Van Gysel, M., Ceulemans, R.: Clonal variation in heavy metal accumulation and biomass production in a poplar coppice culture: II. Vertical distribution and phytoextraction potential. - Environ. Pollut. 133: 541-551, 2005. Go to original source...
    19. Lin, J., Jach, M.E., Ceulemans, R.: Stomatal density and needle anatomy of Scots pine (Pinus sylvestris) are affected by elevated CO2. - New Phytol. 150: 665-674, 2001. Go to original source...
    20. Lunáčková, L., Šottníková, A., Masarovičová, E., Lux, A., Streško, V.: Comparison of cadmium effect on willow and poplar in response to different cultivation conditions. - Biol. Plant. 47: 403-411, 2003/4.
    21. Marchi, S., Tognetti, R., Minnocci, A., Borghi, M., Sebastiani, L.: Variation in mesophyll anatomy and photosynthetic capacity during leaf development in a deciduous mesophyte fruit tree (Prunus persica) and an evergreen sclerophyllous Mediterranean shrub (Olea europaea). - Trees 22: 559-571, 2008. Go to original source...
    22. Markovska, Y.K., Gorinova, N.I., Nedkovska, M.P., Miteva, K.M.: Cadmium-induced oxidative damage and antioxidant responses in Brassica juncea plants. - Biol. Plant. 53: 151-154, 2009. Go to original source...
    23. Marschner, H. (ed.): Mineral Nutrition of Higher Plants. 2nd Ed. - Academic Press, London 1995.
    24. Matyssek, R., Günthardt-Goerg, M.S., Schmutz, P., Saurer, M., Landolt, W., Bucher, J.B.: Response mechanisms of birch and poplar to air pollutants. - J. Sustain. Forest. 6: 3-22, 1998.
    25. Minnocci, A., Panicucci, A., Sebastiani, L., Lorenzini, G., Vitagliano, C.: Physiological and morphological responses of olive plants to ozone exposure during a growing season. - Tree Physiol. 19: 391-397, 1999. Go to original source...
    26. Monclus, R., Dreyer, E., Villar, M., Delmotte, F.M., Delay, D., Petit, J.-M., Barbaroux, C., Le Thiec, D., Bréchet, C., Brignols F.: Impact of drought on productivity and water use efficiency in 29 genotypes of Populus deltoides × Populus nigra.- New Phytol. 169: 765-777, 2006. Go to original source...
    27. Mourato, M.P., Martins, L.L., Campos-Andrada, M.P.: Physiological responses of Lupinus luteus to different copper concentrations. - Biol. Plant. 53: 105-111, 2009. Go to original source...
    28. Scebba, F., Arduini, L., Ercoli, L., Sebastiani, L.: Cadmium effects on growth and antioxidant enzymes activities in Miscanthus sinensis. - Biol. Plant. 50: 688-692, 2006. Go to original source...
    29. Sebastiani, L., Scebba, F., Tognetti, R.: Heavy metal accumulation and growth responses in poplar Eridano (Populus deltoides × maximowiczii) and I-214 (P. × euramericana) exposed to industrial waste. - Environ. exp. Bot. 52: 79-88, 2004. Go to original source...
    30. Shah, K., Nongkynrih, J.M.: Metal hyperaccumulation and bioremediation. - Biol. Plant. 51: 618-634, 2007. Go to original source...
    31. Sims, R.E.H., Hastings, A., Schlamadinger, B., Taylor, G., Smith, P.: Energy crops: current status and future prospects. - Global Change Biol. 12: 2054-2076, 2006. Go to original source...
    32. Soares, A.S., Driscoll, S.P., Olmos, E., Harbinson, J., Arrabaça, M.C., Foyer, C.H.: Adaxial/abaxial specification in the regulation of photosynthesis and stomatal opening with respect to light orientation and growth with CO2 enrichment in the C4 species Paspalum dilatatum. - New Phytol. 177: 186-198, 2007. Go to original source...
    33. Taiz, L., Zeiger, E.: Mineral nutrition. - In: Taiz, L., Zeiger, E. (ed.): Plant Physiology. 2nd Ed. Pp. 103-124. Sinauer Associates, Sunderland 1998.
    34. Terashima, I., Miyazawa, S.I., Hanba, Y.T.: Why are sun leaves thicker than shade leaves? Consideration based on analyses of CO2 diffusion in the leaf. - J. Plant Res. 114: 93-105, 2001. Go to original source...
    35. Tognetti, R., Sebastiani, L., Minnocci, A.: Gas exchange and foliage characteristics of two poplar clones grown in soil amended with industrial waste. - Tree Physiol. 24: 75-82, 2004. Go to original source...
    36. Tuskan, G.A., Di Fazio, S., Jansson, S., Bohlmann, J., Grigoriev, I., Hellsten, U., Putnam, N., Ralph, S., Rombauts, S., Salamov, A., Schein, J., Sterck, L., Aerts, A., Bhalerao, R.R., Bhalerao, R.P., Blaudez, D., Boerjan, W., Brun, A., Brunner, A., Busov, V., Campbell, M., Carlson, J., Chalot, M., Chapman, J., Chen, G.-L., Cooper, D., Coutinho, P.M., Couturier, J., Covert, S., Cronk, Q., Cunningham, R., Davis, J., Degroeve, S., Déjardin, A., De Pamphilis, C., Detter, J., Dirks, B., Dubchak, I., Duplessis, S., Ehlting, J., Ellis, B., Gendler, K., Goodstein, D., Gribskov, M., Grimwood, J., Groover A., Gunter, L., Hamberger, B., Heinze, B., Helariutta Y., Henrissat B., Holligan D., Holt R., Huang W., Islam-Faridi, N., Jones, S., Jones-Rhoades, M., Jorgensen, R., Joshi, C., Kangasjärvi, J., Karlsson, J., Kelleher, C., Kirkpatrick R., Kirst M., Kohler A., Kalluri U., Larimer F., Leebens-Mack, J., Leplé, J.-C., Locascio, P., Lou, Y., Lucas, S., Martin, F., Montanini, B., Napoli, C., Nelson, D.R., Nelson, C., Nieminen, K., Nilsson, O., Pereda, V., Peter, G., Philippe, R., Pilate, G., Poliakov, A., Razumovskaya J., Richardson P., Rinaldi C., Ritland K., Rouzé, P., Ryaboy, D., Schmutz, J., Schrader, J., Segerman, B., Shin, H., Siddiqui, A., Sterky, F., Terry A., Tsai C.-J., Uberbacher E., Unneberg P., Vahala J., Wall, K., Wessler, S., Yang, G., Yin, T., Douglas, C., Marra, M., Sandberg, G., Van de Peer, Y., Rokhsar, D.: The genome of black cottonwood, Populus trichocarpa (Torr. & Gray). - Science 313: 1596-1604, 2006. Go to original source...
    37. Wang, H., Liu, R.L., Jin, J.Y.: Effects of zinc and soil moisture on photosynthetic rate and chlorophyll fluorescence parameters of maize. - Biol. Plant. 53: 191-194, 2009. Go to original source...
    38. Wieser, G., Hecke, K., Tausz, M., Häberle, K.H., Grams, T.E.E., Matyssek, R.: The influence of microclimate and tree age on the defense capacity of European beech (Fagus sylvatica L.) against oxidative stress. - Ann. Forest Sci. 60: 131-135, 2003. Go to original source...
    39. Wójcik, M., Tukiendorf, A.: Cadmium uptake, localization and detoxification in Zea mays. - Biol. Plant. 49: 237-245, 2005. Go to original source...

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