Biologia plantarum 2010, 54:530-534 | DOI: 10.1007/s10535-010-0093-0

Antioxidative system in maize roots as affected by osmotic stress and different nitrogen sources

M. Vuletić1,*, V. Hadži-Tašković Šukalović2, K. Marković1, J. Dragišić Maksimović2
1 Maize Research Institute, Zemun Polje, Zemun, Serbia
2 Institute for Multidisciplinary Research, Belgrade, Serbia

The activities of antioxidative enzymes and contents of proline and total phenolics were assayed in roots of two maize (Zea mays L.) genotypes grown in a medium containing nitrate (NO3 -) or both nitrogen forms, nitrate and ammonium (NH4 +/NO3 -). An increase in the activities of class III peroxidases (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX), ascorbate oxidase (AO) and proline content, and decrease in phenolic content were observed in NH4 +/NO3 - in comparison with NO3 - grown plants. When polyethylene glycol (PEG) was added to both nitrogen treatments, the content of total phenolics and proline was increased, especially in NH4 +/NO3 - treatment. The PEG treatment decreased enzyme activities in NH4 +/NO3 - grown plants, but in NO3 - grown plants activities of POD and SOD were increased, opposite to decreased APX and AO. Isoelectric focusing demonstrated increased activities of acidic POD isoforms in PEG treated NO3 - grown plants, and lower activities of both, acidic and basic isoforms in NH4 +/NO3 -grown plants.

Keywords: antioxidative enzymes; proline; phenolics; Zea mays L.
Subjects: ascorbate oxidase; ascorbate peroxidase; maize; nitrogen supply; osmotic stress; peroxidase; phenolics; polyethylene glycol; proline; reactive oxygen species (ROS); superoxide dismutase (SOD); water stress; Zea mays

Received: October 19, 2008; Accepted: April 24, 2009; Published: September 1, 2010Show citation

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Vuletić, M., Šukalović, V., Marković, K., & Maksimović, J. (2010). Antioxidative system in maize roots as affected by osmotic stress and different nitrogen sources. Biologia plantarum, 54(3), 530-534. doi: 10.1007/s10535-010-0093-0.
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    References

    1. Bates, L.S., Waldren, S.P., Teare, I.D.: Rapid determination of free proline for water-stress studies. - Plant Soil 39: 205-207, 1973. Go to original source...
    2. Bryant, J.P., Chapin III, F.S., Klein, D.R.: Carbon/nutrient balance of boreal plants in relation to vertebrate herbivory. - Oikos 40: 357-368, 1983. Go to original source...
    3. Domínguez-Valdivia, M.D., Aparicio-Tejo, P.M., Lamsfus, C., Cruz, C., Martins-Loução, M.A., Moran, J.F.: Nitrogen nutrition and antioxidant metabolism in ammonium-tolerant and -sensitive plants. - Physiol. Plant. 132: 359-369, 2008. Go to original source...
    4. Escobar, M.A., Geisler, D.A., Rasmusson, A.G.: Reorganization of the alternative pathways of the Arabidopsis respiratory chain by nitrogen supply: opposing effects of ammonium and nitrate. - Plant J. 45: 775-788, 2006. Go to original source...
    5. Foyer, C.H., Noctor, G.: Redox homeostasis and antioxidant signaling: a metabolic interface between perception and physiological responses. - Plant Cell 17: 1866-1875, 2005.
    6. Hadži-TaškoviĆ Šukalović, V., Vuletić, M.: Properties of maize root mitochondria from plants grown on different nitrogen sources. - J. Plant Physiol. 153: 67-73, 1998.
    7. Hadži-Tašković Šukalović, V., Vuletić, M., Vučinić, Ž.: The role of p-coumaric acid in oxidative and peroxidative cycle of the ionically bound peroxidase of the maize root cell wall. - Plant Sci. 168: 931-938, 2005.
    8. Hagerman, A., Harvey-Mueller, I., Makker, H.P.S.: Quantification of Tannins in the Foliage. A Laboratory Manual. - FAO/IAEA, Vienna 2000.
    9. Lasa, B., Frechilla, S., Aparicio-Tejo, P.M., Lamsfus, C.: Role of glutamate dehydrogenase and phosphoenolpyruvate carboxylase activity in ammonium nutrition tolerance in roots. - Plant Physiol. Biochem. 40: 969-976, 2002. Go to original source...
    10. Lin, C.C., Kao, C.H.: Osmotic stress-induced changes in cell wall peroxidase activity and hydrogen peroxide level in roots of rice seedlings. - Plant Growth Regul. 37: 177-184, 2002. Go to original source...
    11. Liu, Q., Yang, J.L., He, L.S., Li, Y.Y., Zheng, S.J.: Effect of aluminum on cell wall, plasma membrane, antioxidants and root elongation in triticale. - Biol. Plant. 52: 87-92, 2008. Go to original source...
    12. Lowry, O.H., Rosebrogh, N.J., Farr, A.L., Randal, R.J.: Protein measurement with the Folin phenol reagent. - J. biol. Chem. 193: 265-275, 1951.
    13. Matysik, J., Alia, Bhalu, B., Mohanty, P.: Molecular mechanisms of quenching of reactive oxygen species by proline under stress in plants. - Curr. Sci. 82: 525-532, 2002.
    14. Penel, C., Castillo, F.J.: Peroxidases of plant plasma membranes, apoplastic ascorbate, and relation of redox activities to plant pathology. - In: Crane, F.L., Morré, D.J., Loew, H. (ed.): Oxidoreduction at the Plasma Membrane. Vol. II. Pp. 121-147. CRC Press, Boca Raton 1991.
    15. Polesskaya, O.G., Kashirina, E.I., Alekhina, N.D.: Changes in the activity of antioxidant enzymes in wheat leaves and roots as a function of nitrogen source and supply. - Russ. J. Plant Physiol. 51: 615-620, 2004. Go to original source...
    16. Polesskaya, O.G., Kashirina, E.I., Alekhina, N.D.: Effect of salt stress on antioxidant system of plants as related to nitrogen nutrition. - Russ. J. Plant Physiol. 53: 186-192, 2006. Go to original source...
    17. Ralph, J., Bunzel, M., Marita, J.M., Hatfield, R.D., Lu, F., Kim, H., Schatz, P.F., Grabber, J.H., Steinhart, H.: Peroxidase-dependent cross-linking reactions of p-hydroxycinnamates in plant cell walls. - Phytochem. Rev. 3: 79-96, 2004. Go to original source...
    18. Rao, M.V., Palyath, G., Ormrod, D.P.: Ultraviolet-B- and ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana. - Plant Physiol. 110: 125-136, 1996. Go to original source...
    19. Reichardt, P.B., Chapin III, F.S., Bryant, J.P., Mattes, B.R., Clausen, T.P.: Carbon/nutrient balance as a predictor of plant defense in Alaskan balsam poplar: potential importance of metabolite turnover. - Oecologia 88: 401-406, 1991. Go to original source...
    20. Rice-Evans, C.A., Nicholas, J.M., Paganga, G.: Antioxidant properties of phenolic compounds. - Trends Plant Sci. 2: 152-159, 1997. Go to original source...
    21. Rios-Gonzales, K., Erdei, L., Lips, S.H.: The activity of antioxidant enzymes in maize and sunflower seedlings as affected by salinity and different nitrogen sources. - Plant Sci. 162: 923-930, 2002. Go to original source...
    22. Rivero, R.M., Ruiz, J.M., Romero, L.M.: Importance of N source on heat stress tolerance due to the accumulation of proline and quaternary ammonium compounds in tomato plants. - Plant Biol. 6: 702-707, 2004. Go to original source...
    23. Sánchez, E., Ruiz, J.M., Romero, L.: The response of proline metabolism to nitrogen deficiency in pods and seeds of French bean (Phaseous vulgaris L. cv. Strike) plants. - J. Sci. Food Agr. 81: 1471-1475, 2001. Go to original source...
    24. Schortemeyer, M., Stamp, P., Feil, B.: Ammonium tolerance and carbohydrate status in maize cultivars. - Ann. Bot. 79: 25-30, 1997. Go to original source...
    25. Skopelitis, D.S., Paranychianakis, N.V., Paschalidis, K.A., Pliakonis, E.D., Delis, I.D., Yokoukakis, D.I., Kouvarakis, A., Papadakis, A.K., Stephanou, E.G., Roubelakis-Angelakis, K.A.: Abiotic stress generates ROS that signal expression of anionic glutamate dehydrogenases to form glutamate for proline synthesis in tobacco and grapevine. - Plant Cell 18: 2767-2781, 2006. Go to original source...
    26. Sutherland, M.W., Learmonth, B.A.: The tetrazolium dyes MTS and XTT provide new quantitative assays for superoxide and superoxide dismutase. - Free Rad. Res. 27: 283-289, 1997. Go to original source...
    27. VeljoviĆ-JovanoviĆ, S., Kukavica, B., StevanoviĆ, B., Navari-Izzo, F.: Senescence- and drought-related changes in peroxidase and superoxide dismutase isoforms in leaves of Ramonda serbica. - J. exp. Bot. 57: 1759-1768, 2006. Go to original source...
    28. Verslues, P.E., Sharp, R.E.: Proline accumulation in maize (Zea mays L.) primary roots at low water potentials. II. Metabolic source increased deposition in the elongation zone. - Plant Physiol. 119: 1349-1360, 1999. Go to original source...
    29. Voetberg, G.S., Sharp, R.E.: Growth of the maize primary root at low water potentials. III. Role of increased proline deposition in osmotic adjustment. - Plant Physiol. 96: 1125-1130, 1991. Go to original source...

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