Biologia plantarum 2016, 60:741-748 | DOI: 10.1007/s10535-016-0602-x

Sucrose-metabolizing enzymes and their genes in the arils of two Dimocarpus longan cultivars

L. Shuai1,2,4, J. Li1,4, J. J. Niu1,4, P. H. Qian1,4, W. H. Liu1,4, X. Q. Xue1,4, D. M. Han3,*, Z. X. Wu1,4,*
1 College of Horticulture, South China Agricultural University, Guangzhou, P.R. China
2 Institute of Food Science and Engineering Technology, Hezhou University, Guangxi, P.R. China
3 Institute of Fruit Tree Research, Guangdong Academy of Agricultural Science, Guangzhou, P.R. China
4 Guangdong Provincal Key Laboratory for Post-harvest Science and the Technology of Fruit and Vegetables, Guangzhou, P.R. China

This study aimed to investigate sucrose-metabolizing enzymes and their genes in fruits of two longan (Dimocarpus longan Lour) cultivars Cihezhong (CHZ) and Lidongben (LDB). Content of sucrose, glucose, and fructose were measured by high-performance liquid chromatography. The genes of sucrose-metabolizing enzymes were cloned by combining reverse transcription polymerase chain reaction and rapid amplification of cDNA ends, and enzyme activities were analyzed at various points in the fruiting cycle. The total soluble solid (TSS) content of longan arils rose and was positively correlated with sucrose content during maturation and then declined as the fruit senesced. Cihezhong showed a more rapid decrease in sucrose content than LDB. The activities of both sucrose phosphate synthase (SPS) and sucrose synthase (SS) were lower in CHZ, whereas the activities of soluble acid invertase (SAI) and neutral invertase (NI) were higher. The full-length cDNA of the genes of the sucrose-metabolizing enzymes were cloned successfully. The patterns of changes of sucrose synthase-2 (DlSS-2), sucrose synthase-3 (DlSS-3), and neutral invertase-3 (DlNI-3) gene expressions corresponded to those of SS and NI activities. The rate of sucrose decline in the longan fruits was related to sugar receding, sucrose metabolizing enzyme activities, and corresponding gene expressions.

Keywords: gene expression; invertase; longan; sucrose phosphate synthase; sucrose synthase
Subjects: sucrose metabolism; gene expression; invertase; sucrose phosphate synthase; sucrose synthase; fruit development; soluble proteins; longan arils
Species: Dimocarpus longan

Received: June 17, 2015; Revised: December 30, 2015; Accepted: January 21, 2016; Published: December 1, 2016Show citation

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Shuai, L., Li, J., Niu, J.J., Qian, P.H., Liu, W.H., Xue, X.Q., Han, D.M., & Wu, Z.X. (2016). Sucrose-metabolizing enzymes and their genes in the arils of two Dimocarpus longan cultivars. Biologia plantarum, 60(4), 741-748. doi: 10.1007/s10535-016-0602-x.
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    References

    1. Beckles, D.M.: Factors affecting the postharvest soluble solids and sugar content of tomato (Solanum lycopersicum L.) fruit. -Postharvest Biol. Tech. 63: 129-140, 2012. Go to original source...
    2. Bieniawska, Z., Paul Barratt, D.H., Garlick, A.P., Thole, V., Kruger, N.J., Martin, C., Zrenner, R., Smith, A.M.: Analysis of the sucrose synthase gene family in Arabidopsis. - Plant J. 49: 810-828, 2007. Go to original source...
    3. Botha, F.C., Black, K.G.: Sucrose phosphate synthase and sucrose synthase activity during maturation of internodal tissue in sugarcane. - Funct. Plant Biol. 27: 81-85, 2000. Go to original source...
    4. Coleman, H.D., Yan, J., Mansfield, S.D.: Sucrose synthase affects carbon partitioning to increase cellulose production and altered cell wall ultrastructure. - Proc. nat. Acad. Sci. USA 106: 13118-13123, 2009. Go to original source...
    5. Dali, N., Michaud, D., Yelle, S.: Evidence for the involvement of sucrose phosphate synthase in the pathway of sugar accumulation in sucrose-accumulating tomato fruits. - Plant Physiol. 99: 434-438, 1992. Go to original source...
    6. Fernie, A.R., Willmitzer, L., Trethewey, R.N.: Sucrose to starch: a transition in molecular plant physiology. - Trends Plant Sci. 7: 35-41, 2002. Go to original source...
    7. Hu, Z., Li, J., Wang, H.: Analysis of fruit sugar and acid compositions in the aril of different longan cultivars. - J. Fruit Sci. 4: 19-22, 2006.
    8. Hubbard, N.L., Huber, S.C., Pharr, D.M.: Sucrose phosphate synthase and acid invertase as determinants of sucrose concentration in developing muskmelon (Cucumis melo L.) fruits. - Plant Physiol. 91: 1527-1534, 1989. Go to original source...
    9. Itai, A., Tanahashi, T.: Inhibition of sucrose loss during cold storage in Japanese pear (Pyrus pyrifolia Nakai) by 1-MCP. - Postharvest Biol. Tech. 48: 355-363, 2008. Go to original source...
    10. King, S.P., Lunn, J.E., Furbank, R.T.: Carbohydrate content and enzyme metabolism in developing canola siliques. - Plant Physiol. 114: 153-160, 1997. Go to original source...
    11. Koch, K.: Sucrose metabolism: regulatory mechanisms and pivotal roles in sugar sensing and plant development. - Curr. Opin. Plant Biol. 7: 235-246, 2004. Go to original source...
    12. Li, M., Feng, F., Cheng, L.: Expression patterns of genes involved in sugar metabolism and accumulation during apple fruit development. - PLoS ONE 7: e33055, 2012. Go to original source...
    13. Lin, Y.L., Lai, Z.X.: Reference gene selection for qPCR analysis during somatic embryogenesis in longan tree. - Plant Sci. 178: 359-365, 2010. Go to original source...
    14. Livak, K.J., Schmittgen, T.D.: Analysis of relative gene expression data using real-time quantitative PCR and the 2ΔΔCT method. - Methods 25: 402-408, 2001. Go to original source...
    15. Menzel, C., Waite, G.: Litchi and Longan: Botany, Production and Uses. - CABI, Wallingford 2005.
    16. Nguyen-Quoc, B., Foyer, C.H.: A role for 'futile cycles' involving invertase and sucrose synthase in sucrose metabolism of tomato fruit. - J. exp. Bot. 52: 881-889, 2001. Go to original source...
    17. Nguyen-Quoc, B., N'Tchobo, H., Foyer, C.H., Yelle, S.: Overexpression of sucrose phosphate synthase increases sucrose unloading in transformed tomato fruit. - J. exp. Bot. 50: 785-791, 1999. Go to original source...
    18. Pan, Q., Yu, X., Zhang, N., Zou, X., Peng, C., Wang, X., Zou, K., Zhang, D.: Activity, but not expression, of soluble and cell wall-bound acid invertases is induced by abscisic acid in developing apple fruit. - J. integr. Plant Biol. 48: 536-549, 2006. Go to original source...
    19. Park, J., Canam, T., Kang, K., Unda, F., Mansfield, S.D.: Sucrose phosphate synthase expression influences poplar phenology. - Tree Physiol. 29: 937-946, 2009. Go to original source...
    20. Qazi, H.A., Paranjpe, S., Bhargava, S.: Stem sugar accumulation in sweet sorghum: activity and expression of sucrose metabolizing enzymes and sucrose transporters. - J. Plant Physiol. 169: 605-613, 2012. Go to original source...
    21. Ren, X., Zhang, J.: Research progresses on the key enzymes involved in sucrose metabolism in maize. - Carbohydr. Res. 368: 29-34, 2013. Go to original source...
    22. Xie, Z., Li, B., Forney, C.F., Xu, W., Wang, S.: Changes in sugar content and relative enzyme activity in grape berry in response to root restriction. - Scientia Hort. 123: 39-45, 2009. Go to original source...
    23. Yang, Z., Wang, T., Wang, H., Huang, X., Qin, Y., Hu, G.: Patterns of enzyme activities and gene expressions in sucrose metabolism in relation to sugar accumulation and composition in the aril of Litchi chinensis Sonn. - J. Plant Physiol. 170: 731-740, 2013. Go to original source...
    24. Yonemoto, Y., Chowdhury, A.K., Kato, H., Macha, M.M.: Cultivars identification and their genetic relationships in Dimocarpus longan subspecies based on RAPD markers. - Scientia Hort. 109: 147-152, 2006. Go to original source...
    25. You, F.M., Huo, N., Gu, Y.Q., Luo, M., Ma, Y., Hane, D., Lazo, G.R., Dvorak, J., Anderson, O.D.: BatchPrimer3: a high throughput web application for PCR and sequencing primer design. - BMC Bioinformatics 9: 253, 2008. Go to original source...
    26. Zhang, C., Shen, Z., Zhang, Y., Han, J., Ma, R., Korir, N.K., Yu, M.: Cloning and expression of genes related to the sucrose-metabolizing enzymes and carbohydrate changes in peach. - Acta Physiol. Plant. 35: 589-602, 2013. Go to original source...
    27. Zhang, X.M., Dou, M.A., Yao, Y.L., Du, L.Q., Li, J.G., Sun, G.M.: Dynamic analysis of sugar metabolism in different harvest seasons of pineapple [Ananas comosus L. (Merr.)]. - Afr. J. Biotechnol 10: 2716-2723, 2010.
    28. Zhong, H., Chen, J., Li, C., Chen, L., Wu, J., Chen, J., Lu, W., Li, J.: Selection of reliable reference genes for expression studies by reverse transcription quantitative real-time PCR in litchi under different experimental conditions. - Plant Cell Rep. 30: 641-653, 2011. Go to original source...
    29. Zhu, Y.J., Komor, E., Moore, P.H.: Sucrose accumulation in the sugarcane stem is regulated by the difference between the activities of soluble acid invertase and sucrose phosphate synthase. - Plant Physiol. 115: 609-616, 1997. Go to original source...
    30. Zrenner, R., Salanoubat, M., Willmitzer, L., Sonnewald, U.: Evidence of the crucial role of sucrose synthase for sink strength using transgenic potato plants (Solanum tuberosum L.). - Plant J. 7: 97-107, 1995. Go to original source...

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