"Regulation of Genome Expression in Plants"

Biologia plantarum 24:321, 1982 | DOI: 10.1007/BF02909094

Book review

Jarmila Solárová, Jarmila Solárová, M. Kamínek

Biologia plantarum 24:399-400, 1982 | DOI: 10.1007/BF02909111

Nicotiana tabacum DNA: Isolation from cell culture

J. Šatava, Z. Opatrný

Biologia plantarum 24:322-325, 1982 | DOI: 10.1007/BF02909095

The adaptation of Marmur's method, suitable for DNA isolation from plant cell culture, is described.

Selective inhibition by arabinosylcytosine of oligo(U)-containing RNA synthesis in germinating wheat embryos

M. Tomaszewski

Biologia plantarum 24:326, 1982 | DOI: 10.1007/BF02909097

Arabinosylcytosine (araC) was found to influence the pattern of RNA labelling in early wheat embryos. Although the rate of uridine-¹⁴C incorporation into total RNA remained unchanged, specific radioactivity of an oligo(U)-containing subclass of nonpolyadenylated RNA was markedly lower (50%) in araC-treated (1.5 mM, 1 h) than in control embryos. The inhibitory effect was stronger for nuclear than for cytoplasmic subpopulation of these RNA species. h labelling of all other RNA fractions, including bulk nonpolyadenylated RNA as well as poly(A)-containing RNA of both nuclear and cytoplasmic origins, did not respond to the presence of araC.

Alterations in polyadenylated RNA during pollen maturation and germination

J. Tupý

Biologia plantarum 24:331, 1982 | DOI: 10.1007/BF02909098

Developmental changes in poly(A)-bearing RNA in male tobacco gametophyte were examined by sedimentation analysis and by hybridization with³H-poly(U). The results indicate that the transition of microspore undergoing postmeiotic division to mature pollen is accompanied by characteristic changes in RNA and poly(A) content and the size of poly(A)⁺RNA.
The volume of pollen grain increases about 2times, total RNA per grain from 34 to 230 pg and poly(A) from 22 to 450 fg, which together with the estimated increase in the number average size of poly(A)⁺RNA from 700 to 2 100 nucleotides suggests an approx. rise of RNA containing...

Effect of irradiance on the course of RNA synthesis in the cell cycle ofScenedesmus quadricauda

V. Zachleder, I. Šetlík

Biologia plantarum 24:341, 1982 | DOI: 10.1007/BF02909100

In synchronous populations ofScenedesmus quadricauda the RNA amount in the cells increases in waves: periods of a high rate of RNA synthesis alternate with periods of a low rate in the course of the cell cycle. Each wave usually leads to the doubling of the RNA amount per cell. In cells growing under normal conditions the waves of RNA synthesis seem to be linked with consecutive rounds of DNA replication. The pattern of RNA synthesis in the course of the cell cycle, however, does not change, if DNA replication is prevented by application of 5-fluoro-deoxyuridine. In darkness the rate of RNA synthesis drops to zero and thereafter the RNA amount...

Synthesis of proteins, RNA and DNA in dormant and after-ripenedCaryophyllaceae seeds

K. -H. Köhler, U. Müller, M. Hecker, D. Bernhardt

Biologia plantarum 24:354, 1982 | DOI: 10.1007/BF02909102

Macromolecule syntheses, especially incorporation of radioactive labelled precursors into proteins, RNA and DNA were investigated. Some results on the action of phytohormones applied to dormant seeds and on the influence on water stress conditions by interruption of imbibition even before the radicle protrudes, on germination as well as on RNA and DNA synthesis were analysed. Benzylaminopurine and ethylene, applied in combination, could break dormancy of dormant seeds; a process which is correlated with the onset of DNA synthesis. Interruption of the imbibition during the time of onset of DNA synthesis (after 16 h of imbibition) did not impair the...

Distinctive characteristics of RNA and protein synthesis in pea cotyledons at early stages of germination

N. A. Gumilevskaya, M. A. Skazhennik, A. T. Akhmatova, L. V. Chumikina, E. B. Kulaeva, V. L. Kretovich

Biologia plantarum 24:363-373, 1982 | DOI: 10.1007/BF02909104

The RNA and protein synthesis in pea cotyledons at early imbibition were studied. The results obtained show that RNA and protein synthesis are reactivated at the beginning of imbibition. The initial transcription is independent of protein synthesis, after 8 h synthesis RNA depends on translation by 44 - 52%. α-Amanitin (5μg ml^-1) inhibits³H-uridine incorporation by 75%. It indicates the predominance of mRNA synthesis in early transcription.
The protein synthesis during first hours of imbibition depends significantly (by 50%) on RNA synthesis. It means newly-made mRNA is involved in the translation. Protein synthesis,...

Cell-free translation of polyribosomes from detached pumpkin cotyledons: Effects of starvation and cytokinin

N. L. Klyachko, L. A. Yakovleva, F. M. Shakirova, O. N. Kulaeva

Biologia plantarum 24:374, 1982 | DOI: 10.1007/BF02909106

The effects of 6-benzylaminopurine (BAP, 5.10^-5M) treatment of pumpkin cotyledons and their starvation after excision upon polysome/monosome ratio and translational capacity of polysomes in cell-free system were studied. It has been found that starvation causes a progressive polysome degradation. Polysome translation in a wheat germ cell-free proteinsynthesizing system reveals that the translation capacity of polysome preparations decreases with the time after cotyledon excision much more sharply than polysome/monosome ratio. This indicates the starvation damage in elongation steps of protein synthesis. The decrease of postribosomal supernatants...

Attempts to characterize particular parts of the root tip on the basis of isoenzyme patterns of various glycosidases

K. Benes, Vera Hadacová

Biologia plantarum 24:381, 1982 | DOI: 10.1007/BF02909107

Using polyacryl amide gel electrophoresis isoenzyme patterns were studied of α- and β-glucosidase, α- and β-galactosidase, α-fucosidase, β-glucosaminidase, β-glucuronidase, α-man-nosidase and β-xylosidase in whole root tips, in particular growth zones and in the cortex and central cylinder of broad bean and maize. Though considerable differences even in number and position of isoenzymes were found in particular cases in both objects, no general conclusion could be reached. The mentioned differences are not obligatory and have been observed both between zones of division and elongation and between zones...

Acid-soluble chromosomal proteins in maize root and callus cells and after rhizogenesis induction in callus tissues

Stanka Koleva, Elena Marinova, Stefka Varadinova, Elena Tsikova, A. Atanassov

Biologia plantarum 24:388, 1982 | DOI: 10.1007/BF02909109

Callus tissues originating fromZea mays root meristem, induced for rhizogenesis callus, meristematic and differentiated maize root cells for isolation of nuclei and acid-soluble chromosomal proteins were used. Cytological investigations proved that rhizogenesis begins with the formation of meristematic centres, followed by root differentiation about 5-12 days after the treatment with α-naphtalene acetic acid (NAA). When applying electrophoresis in 15% polyacrylamide gel, differences between the electrophoretic profiles of acid-soluble chromosomal proteins, isolated from root cells and from callus tissues, were established. The main differences...

Comparative studies on the electrophoretic patterns of acid-soluble chromosomal proteins duringZea mays early stages of embryo germination and root cell differentiation

Elesa Marinova, Stanka Koleva

Biologia plantarum 24:394, 1982 | DOI: 10.1007/BF02909110

Acid-soluble chromosomal proteins were extracted from purified nuclei, isolated from 3 - 4 h, 12 -14 h and 24 -26 h maize embryos, as well as from nuclei isolated from meris-tematic, elongating and differentiated cells, from 2 and 3-day-oldZea mays seedlings primary roots.
Using polyacrylamide gel electrophoresis (urea-acetic acid in the cylindrical gels and slab-SDS-electrophoresis), it was established that germination and root cell differentiation induced changes in some nuclear acid-soluble chromosomal proteins, especially in histone H1.
The results of proteins belonging to the high-mobility group proteins (HMG) and some other acid-soluble...

Proteines foljaires et alimentation

J. Zima

Biologia plantarum 24:325, 1982 | DOI: 10.1007/BF02909096

The molecular biology of the yeast saccharomyces

T. Gichner

Biologia plantarum 24:340, 1982 | DOI: 10.1007/BF02909099

Stochastic nonlinear systems in physics, chemistry, and biology

Vera Lánská

Biologia plantarum 24:353, 1982 | DOI: 10.1007/BF02909101

Gene Amplification

T. Gichner

Biologia plantarum 24:362, 1982 | DOI: 10.1007/BF02909103

Water-in-plants bibliography

Hanno Richter

Biologia plantarum 24:373, 1982 | DOI: 10.1007/BF02909105

Plants and mineral salts

F. Pospísil

Biologia plantarum 24:387, 1982 | DOI: 10.1007/BF02909108