Effect of heavy metal ions on growth and biochemical characteristics of photosynthesis of barley and maize seedlings

Marie Stiborová, Marta Ditrichová, Alena Březinová

Biologia plantarum 29:453, 1987 | DOI: 10.1007/BF02882221

The effects of Cu²⁺, Zn²⁺, Cd²⁺ and Pb²⁺ on growth and the biochemical characteristics of photosynthesis were more expressed in barley (Hordeum vulgare L.) than in maize (Zea mays L.) seedlings. The barley and maize seedlings exhibited retardation in shoot and root growth after exposure of Cu²⁺, Cd²⁺ and Pb²⁺. The Zn²⁺ions practically did not influence these characteristics. The total protein content of barley and maize roots declined with an increase in heavy metal ion concentrations. The protein content of barley shoots was only slighly decreased...

Changes in ribosephosphate isomerase and ribosephosphate pyrophosphokinase activities in tobacco infected with PVY

L. Šindelář, Milada Šindelářová

Biologia plantarum 29:468-472, 1987 | DOI: 10.1007/BF02882223

Changes in ribosephosphate isomerase and ribosephosphate pyrophosphokinase activities occurring in tobacco leaf tissues infected with the potato virus Y (PVY) were studied at the stage of acute infection. The results obtained have shown that during the entire experimental period the activities of both enzymes were at the end of a dark phase much higher in virus-infected tissues compared with the values found in healthy control plants. The courses of the activity curves of both enzymes were consistent and correlated with the reproduction curve of PVY. The results obtained suggest a direct involvement of both enzymes inde novo biosynthesis of...

Some immunochemical properties of pollen extracellular nuclease

J. Matoušek, Věra Turková

Biologia plantarum 29:401-408, 1987 | DOI: 10.1007/BF02882205

The extracellular nuclease fromPinus silvestris pollen was isolated and characterized. Molecular sieving produced only one peak of nuclease with apparent molecular mass of about 30 kDaltons, while molecular mass of the enzyme as determined by means of sodium dodecyl sulphate-DNA gel electrophoresis was 25.7 kDaltons or less. The nuclease exhibited one electrophoretic molecular form only.
Specific antiserum against this nuclease was prepared and the nuclease was shown to be immunochemically cross-reactive with extracellular nuclease from Pinus nigra pollen. No immunochemical relationships were observed between pine nuclease and the pollen...

In vitro: Regeneration of strawberry plants from petiole segments and flower buds

Catherine Foucault, R. Letouze

Biologia plantarum 29:409, 1987 | DOI: 10.1007/BF02882207

Strawberry plants have been regenerated from petioles and flower buds Petiole segments derived from strawberry plants(Fragaria vesca L. andFragaria xananassa Duch. cvs. Gorella and Redgauntlet) maintainedin vitro have been cultured on the Gambobg's. agar medium supplemented with 2,4-D (2 mg 1-¹) either alone or with kinetin or BAP (0.1 mg I-¹ or 1 mg I-¹). The calli thus obtained have been transferred to media differing from the first ones only by the nature and the concentration of the growth regulators used. One or two months later, shoots appeared on these calli. The influence of the media...

Photosynthesis of plant régénérants. Specificity ofin vitro conditions and plantlet response

Jana Pospíšilová, J. Čatský, Jarmila Solárová, Ingrid Tichá

Biologia plantarum 29:415, 1987 | DOI: 10.1007/BF02882209

Regenerants from tobacco(Nicotiana tabacum L. cv. White Burley) leaf segments cultivatedin vitro in vessels with solid agar medium under usual conditions (plantlets) grew under very low irradiance (I = 40 μxmol m-2 s-1), very high relative humidity (more than 90%) and decreased CO₂ concentration (c_a) during light period. In comparison with seedlings of a similar number of leaves and similar total leaf area grown in sand and nutrient solution, the plantlets had lower dry mass of shoots and roots per plant and thinner leaves almost without trichomes and epicuticular waxes. Due to a low transpiration rate...

The separation of two cell strains with different secondary substance production by means of an improved adsorbent filter technique

J. Thumann, B. Knoop, R. Beiderbeck

Biologia plantarum 29:422-424, 1987 | DOI: 10.1007/BF02882211

This paper describes the successful selection of plant cell var ants with differing secondary substance productions by means of an adsorbent filter method. The method allows a simultaneous screening of up to 100 small cell aggregates per Petri dish within short time.

Effect of benzylaminopurine and thidiazuron onin vitro shoot proliferation ofTilia cordata MILL.,Sorbus aucuparia L. andRobinia pseudoacacia L.

V. Chalupa

Biologia plantarum 29:425-429, 1987 | DOI: 10.1007/BF02882213

Benzylaminopurine and thidiazuron stimulated shoot proliferation ofTilia, Sorbus andRobinia. Low concentration of BAP (0.2-1.0 mg I^-1) promoted axillary bud formation and shoot elongation. Thidiazuron displayed high cytokinin activity at very low concentrations (0.002-0.05 mg I^-1). Shoot number induced on media containing thidiazuron was large. Numerous shoots were produced on the media containing BAP together with thidiazuron. Shoots produced on media containing thidiazuron or BAP together with thidiazuron rooted after transfer to medium supplemented with low concentration of auxin (IBA or NAA).

Red light irradiation ofWolffia arrhiza does not influence transcription activity of isolated DNA-dependent RNA polymerase II

K. -J. Appenroth, H. Augsten

Biologia plantarum 29:430, 1987 | DOI: 10.1007/BF02882215

DNA-dependent RNA polymerase II (nucleosidetriphosphate: RNA nucleotidyltransferase, EC 2.7.7.6.) from duckweed Wolffia arrhiza (L.) HORKEL ex WIMMER was partially purified by polyethylenimine precipitation and DEAE cellulose ion exchange chromatography. Whole plants were irradiated with red light pulses. After a dark period of 2 to 12 h there is no effect of light pretreatment on the total or specific activity of the purified enzyme.

Formation of adventitious buds in needle primordia isolated from embryonic shoots of adult picea abies

P. Krogstrup

Biologia plantarum 29:437-444, 1987 | DOI: 10.1007/BF02882217

Needle primordia were isolated from embryonic shoot expiants of 88 year oldPicea abies (Norway spruce) in order to develop a method for micropropagation using adult mother trees. The isolation of needle primordia in combination with frequent subculture, stimulated the formation of peripheral meristematic tissue in expiants cultured on a modified Murashige & Skoog medium supplemented with cytokinin. Transfer of isolated needle primordia with peripheral meristematic tissue to medium without growth regulators, and with a reduced ratio of NH+4/ NO-3, resulted in the formation of adventitious buds.

Biochemical changes in primary wheat leaves during growth and senescence

H. Miidla, E. Padu, Ü. Kolk, A. Soosaar

Biologia plantarum 29:445, 1987 | DOI: 10.1007/BF02882219

Changes in the different forms of the total activity of peroxidases (soluble, ionically and covalently bound), in the soluble isoperoxidase composition, and in the content of protein and chlorophyll during growth and senescence of the intact primary leaves of spring wheat were studied. The forms of peroxidases are in inverse relationship to the growth and increase during development and senescence, especially towards the end of senescence. The activity of peroxidases does not follow the physiological age of wheat leaves during the whole period of vegetation unlike the contents of protein and chlorophyll. The intensity of anodic and cathodic peroxidase...

Horgan, R., Jeffcoat, B. (ed.): Cytokinins: PlantHormones in Sbabch of a Role

Libuše Pavlová

Biologia plantarum 29:408, 1987 | DOI: 10.1007/BF02882206

Kendrick, R. E., Kronenberg, G. H. M. (ed.): Photomorphogenesis in Plants

Libuše Pavlová

Biologia plantarum 29:414, 1987 | DOI: 10.1007/BF02882208

Sharma, S. K., Gupta, C.: Saline Environment and Plant Growth

Jana Pospíšilová

Biologia plantarum 29:421, 1987 | DOI: 10.1007/BF02882210

IARC Monographs on the Evaluation of the Carcinogenic Risk of Chemicals to Humans

J. Velemínský

Biologia plantarum 29:424, 1987 | DOI: 10.1007/BF02882212

Miller, J. R., Miller, T. A. (ed.): Insect-Plant Interactions. - Springer Series in Experimental Entomology

Helena Havlíčková

Biologia plantarum 29:429, 1987 | DOI: 10.1007/BF02882214

Moore, R. (ed.): Vegetative Compatibility Responses in Plants

Vlasta Čatská

Biologia plantarum 29:436, 1987 | DOI: 10.1007/BF02882216

Sestak, Z., Catsky, J. (ed.): Photosynthesis Bibliography

J. P. Gaudillère

Biologia plantarum 29:444, 1987 | DOI: 10.1007/BF02882218

IARC Monographs on the Evaluation of the Carcinogenic Risk of Chemicals to Humans

T. Gichner

Biologia plantarum 29:452, 1987 | DOI: 10.1007/BF02882220

Hu Han, Yang Hongyuan (ed.): Haploids of Higher Plants in vitro

V. Žárský

Biologia plantarum 29:467, 1987 | DOI: 10.1007/BF02882222

Govindjee, Barber, J., Cramer, W. A., Goedheer, J. H. C., Lavorel, J., Marcelle, R., Zilinskas, B. (ed.): Excitation Energy and Electron Transfer in Photosynthesis

I. Šetlík, Jarmila Solárová, Vlasta Čatská, Eva Zažímalová, Ivana Macháčková

Biologia plantarum 29:476-480, 1987 | DOI: 10.1007/BF02882225

Biochemical indicators of water stress in sunflower seedlings

A. L. García, A. Torrecillas, A. León, M. J. Sánchez-Blanco

Biologia plantarum 29:473, 1987 | DOI: 10.1007/BF02882224

The free proline and chlorophyll contents, and the chlorophyllase, peroxidase and nitrate-reductase activities were determined in sunflower seedlings grown under controlled conditions and submitted to water stress induced by 14 % polyethyleneglycolj (M_r = 4000) or isotonic NaCl solution. Combined free proline content and peroxidase activity may be used for detection of the factor inducing water stress.