Effect of osmotic stress on transpiration and absorption rates in triticale and its parental species

A. Morant-Avice, G. Ferard, A. Coudret

Biologia plantarum 31:241, 1989 | DOI: 10.1007/BF02907283

In triticale and its parental species, the application of a root osmotic stress induced either a transient increase or an immediate decrease in transpiration rate. The response of wheat (Trticum dicoccum farrum) proved to depend on relative humidity of air. In rye (Secale cerealecv. Petkus) and triticale (T. 300) the effect of NaCl stress was less expressive, than the effect of PEG.

Studies on lavandin callus cultures: Ethylene production in relation to the growth

Anna Mensuali Sodi, Maddalena Panizza, F. Tognoni

Biologia plantarum 31:247-253, 1989 | DOI: 10.1007/BF02907284

Ethylene production and growth of callus cultures of lavandin (Lavandula offidnalis Cham x Lavandula latifolia Villars) cv. Grosso were examined. Callus lines, derived from various kinds of primary expiants (shoot tip, leaf and calyx), exhibited differences in ethylene production patterns independent of callus growth. Moreover these differences could not be ascribed to the expiant source. Within a line, ethylene pattern paralleled callus growth curve. Variations in ethylene evolution were induced in shoot tip callus by means of ACC, AVG and varied amounts of 2,4-D in the culture medium. Following all these treatments callus growth was not altered....

Influence of light on adventitious root formation in birch shoot culturesin vitro

Ina Pinker, K. Zoglauer, H. Göring

Biologia plantarum 31:254, 1989 | DOI: 10.1007/BF02907285

The influence of light of different spectral composition and levels of irradiance (2-40 Wm-²) on adventitious root formation (ARF) in birch shoot segments was investigated. Spontaneous rooting of shoot segments occurred in segments with intact apical or axillary meristems. Concerning ARF shoot meristems could be substituted by application of auxin. The very low rooting percentage of shoot segments in darkness was improved considerably by auxin application. Irradiation of cuttings was a requirement for a high percentage of spontaneous rooting. The promoting effect of light was dependent on its spectral composition and was the highest under...

Effects of different nitrogen compounds and temperatures on the germination ofAvena sterilis spp.macrocarpa Mo.

R. Gonzalez Ponce, M. L Salas

Biologia plantarum 31:261, 1989 | DOI: 10.1007/BF02907286

The effect of several nitrogen compounds on the germination of the first seed of each spikelet ofAvena sterilis spp.macrocarpa Mo. was studied on Petri dishes placed in germination chamber. The compounds assayed were ammonium sulphate, ammonium nitrate, urea and calcium nitrate, generally used as fertilizer for cereal crops, as well as a control supplied with water only
The concentration of the assayed compounds were 100, 1000, 2500 and 5000 ppm either at a constant temperature of 15 ° C or at alternated temperatures 5°C and 15 °C, for 16 and 8 h, respectively
Significant increase in the germination percentages occurred when nitrate...

Compared effectiveness of antibiotic treatments and shoot tip culture on bacterial decontamination of an in vitro propagated clone of hybrid walnut (Juglans nigra x J. regia)

Veronique Meynier, Marie-France Arnould

Biologia plantarum 31:269-275, 1989 | DOI: 10.1007/BF02907287

Two ways were tested to obtain the definitive bacterial decontamination of a clone of hybrid walnut culturedin vitro: treatment of the plantlets with Rifampicin and other antibiotic agents, and culture establishmentvia shoot tip expiants. The former failed whereas the latter led to plantlets truly free of contaminants which could be satisfactory propagated, rooted and weaned

Histological and histochemical investigations of Pollen embryos ofBrassica juncea (L.) Czern

Kiran K. Sharma, Sant S. Bhojwani

Biologia plantarum 31:276, 1989 | DOI: 10.1007/BF02907288

Pollen embryos of Brassica juncea (L.) Czern., obtained through anther culture, were examined histologically and histochemically to find explanation for their inability to germinate normally like a zygotic embryo. Most of the embryos lacked a distinct plumule or both plumule and radicle. Anotherabnormal feature of these embryos was the degeneration of cells in the hypocotyl and radicle, whichoccurred either through lysis or cell shrinkage. Reserve food material was mainly in the form of proteinbodies and starch grains. Unlike the zygotic embryos, the pollen embryos lacked lipids. It is concluded thatthe inability of the pollen embryos to germinate...

Progeny study of tobacco regenerant transformed by agrobacterium rhizogenes

M. OndRej, M. Hrouda, Anna KoneCnÁ, J. VlasÁk

Biologia plantarum 31:280-285, 1989 | DOI: 10.1007/BF02907289

The pRi T-DNA markers were followed in the first generation of the progeny ofAgrobacterium tumefaciens strain C58Cl(pRiA4b) transformed tobacco regenerant. Two categories segregating probably in the ratio 15: 1 can be distinguished: those showing a complex of pRi T-DNA transformation markers and those showing no transformation markers. The presence of both T_L and T_R-DNA was demonstrated by Southern blotting. The most prominent of the transformation markers observedIn vitro was rapid growth of detached plagiotropic roots on media without phytohormones. Most of the progeny plants of the transformant showed irregularities...

Free auxin level and inheritance of introduced markers in tobacco transformed by binary vector eased on A4 Ri plasmid

M. Ondřej, J. Eder, M. Hrouda, Ivana Macháčková, J. Vlasák

Biologia plantarum 31:286-291, 1989 | DOI: 10.1007/BF02907290

Tobacco cv. Mamont was transformed by leaf disc method with A.tumefaciens C58C1 (pRiA4b) (pGA472). Transformed leaf segments were cultivated on medium with kanamycin as a selection agent, but without phytohormones. Transformed tissues grew as roots which later regenerated plants. Kanamycin in the medium ensured that only doubly transformed tissues, which contain in their genome both pRi T-DNA as well as pGA472 chimeric kanamycin resistance gene were recovered. Regenerated plants showed clear-cut morphological deviations, but only a slight increase of the auxin content. Kanamycin resistance in the progeny segregated in the Mendelian ratio 3: 1 or 2: 1.

The Effect of temperature gradient on the transport phenomenon in roots of maize plants grown under salinity conditions. substance, heat, and ion flows

J. Michalov

Biologia plantarum 31:292, 1989 | DOI: 10.1007/BF02907291

The accumulation of nitrogen and potassium by plant cells at undesirable concentrations manifests itself in changes in the osmotic phenomenon and finally in the transport process. Temperature gradient is another factor influencing the transport phenomenon. We have followed the changes in the electric diffusion potential and the heat transfer under the influence of these two factors on transport properties of roots of plants grown under conditions of either salinity or nutrient deficiency on the transport of KG solution through segments of the first node roots and of the primary root. Physical causes of the accumulation of the above mentioned ions were...

The effect of temperature gradient on the transport phenomenon in roots of maize plants grown under salinity conditions. conductivity and filtration properties

J. Michalov

Biologia plantarum 31:302-311, 1989 | DOI: 10.1007/BF02907292

An analysis of flows through primary root and first node root tissues of plants grown under conditions of salinity and nutrient deficiency induced by temperature gradients was carried out using. a mathematical model. The results obtained show that high KNO₃ concentration in Knop's nutrient solution (salinity) causes an inhibition of volume and heat flows and that the omission of KNO₃ from Knop's nutrient solution (deficiency) stimulates these flows. The causes of the inhibition lay in the fact that salinity reduced hydraulic, electric, and osmotic conductivity when compared with the control (Knop's solution), but relative to nutrient...

Book review

Dagmar Dykyjová, L. Šindelář, J. Gut, F. Pospíšil, J. Velemínský, T. Gichner, C. Záruba, Libše Pavlová

Biologia plantarum 31:314-320, 1989 | DOI: 10.1007/BF02907294

Potato transformation byAgrobacterium rhizogenes Ri plasmid

M. Ondřej, M. Hrouda, P. Kostřica

Biologia plantarum 31:312, 1989 | DOI: 10.1007/BF02907293

Potato root cultures transformed by_pRiA4 T-DNA do not regenerate plants in vitro spontaneously, but regeneration can be induced by a sequence of callogenesis and regeneration media. Both T_l and T_r-DNA were found by Southern blotting. Regenerated transformants showed characteristic morphological deviations in the in_vitro cultures. Plants in the soil showed differences in the inflorescence and tuber development.