Biologia plantarum 2013, 57:449-456 | DOI: 10.1007/s10535-012-0299-4

Cryptic homoeology analysis in species and hybrids of genus Zea

M. del C. Molina1,2,*, C. G. López3, S. Staltari1, S. E. Chorzempa3, V. Moreno Ferrero4
1 Instituto Fitotécnico de Santa Catalina, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de la Plata, Garibaldi, Llavallol, Buenos Aires, Argentina
2 Consejo Nacional de Investigaciones Científicas y Técnicas, Rivadavia, Ciudad Autónoma de Buenos Aires, Argentina
3 Facultad de Ciencias Agrarias, Universidad Nacional de Lomas de Zamora, Buenos Aires, Argentina
4 Departamento de Biotecnología, Universidad Politécnica de Valencia and Instituto de Biología Molecular y Celular de Plantas, Valencia, Spain

Cryptic intergenomic pairing of genus Zea was induced by the use of a diluted colchicine solution in order to elucidate the phylogenetic relations and differentiation of the homoeologous genomes. Results indicate that in species and hybrids with 2n = 20, there was chromosome pairing between the homoeologous A and B genomes with a maximum of 5IV, with the exception of Zea diploperennis and their interspecific hybrids where cryptic homoeologous chromosome pairing was not induced. In almost all 2n = 30 hybrids, observed cryptic pairing increased to a maximum of 10III although Z. mays × Z. mays with 2n = 30 did not show significant differences between treated and untreated materials. Pairing was also observed in species and hybrids with 2n = 40, in which a maximum of 10IV was observed, with the exception of Z. mays with 2n = 40 where treated and untreated cells did not differ significantly.

Keywords: colchicine; genome; maize; subgenome; teosinte
Subjects: cryptic homoeology; hybrids; colchicine; ploidy; chromosomes; evolution; maize
Species: Zea mays; Zea luxurians; Zea diploperennis; Zea perennis

Received: December 2, 2011; Accepted: October 4, 2012; Published: September 1, 2013Show citation

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del Molina, M.C., López, C.G., Staltari, S., Chorzempa, S.E., & Ferrero, V. (2013). Cryptic homoeology analysis in species and hybrids of genus Zea. Biologia plantarum, 57(3), 449-456. doi: 10.1007/s10535-012-0299-4.
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