Biologia plantarum 2014, 58:218-230 | DOI: 10.1007/s10535-013-0383-4

Cullin, a component of the SCF complex, interacts with TaRMD5 during wheat spike development

M. J. Hong1,2, D. Y. Kim1, Y. W. Seo1,*
1 Division of Biotechnology, Korea University, Seoul, Republic of Korea
2 Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeonbuk, Republic of Korea

Cullin, a major component of the SKP1-cullin-F box protein (SCF) complex, is a scaffold protein that binds to both SKP1 and RBX1 for selective protein degradation through the ubiquitin proteasome system. In order to study the role of cullin in common wheat, we isolated TaCullin (Cullin gene from Triticum aestivum) from wheat spike cDNA. TaCullin was expressed during all spike/grain developmental stages and in high amounts during early spike/grain development. The TaCullin gene is located on the chromosome arm 2DL. Our results suggest that unneddylated TaCullin is located in the nucleus. Based on previous proposals of Cullin-SKP1 interactions, we examined the interaction between TaCullin and SKP1-like protein (TaSKP) families by using a yeast two-hybrid approach. Yeast cotransformation demonstrated that the N-terminus of TaCullin physically interacts with TaSKP proteins. Using the yeast two-hybrid screen, we identified potential TaCullin-interacting proteins in a wheat spike library. Among the 9 clones that were identified as potential interacting partners of TaCullin, we identified E3-like ubiquitin ligase, targeting fructose-1,6-bisphosphatase (RMD5) homolog A-like protein. The interaction between TaCullin and the TaRMD5 homolog A-like protein was specifically mediated through the C-terminus of TaCullin. The results of bimolecular fluorescence complementation assay indicated that TaCullin-TaRMD5 is localized in the plasma membrane and cytoplasm. In this study, we present that TaRMD5, such as RING box protein 1 (RBX1), has the potential to interact with TaCullin, depending on the developmental stage and particular organ tissues analyzed.

Keywords: chromosome location; TaSKP; Triicum aestivum; yeast two-hybrid screening
Subjects: Cullin; SCF complex; yeast two-hybrid screening; abscisic acid; auxin; gibberellic acid; methyl jasmonate; temperature - low; NaCl; polyethylene glycol; developmental stages; wheat
Species: Triticum aestivum

Received: January 10, 2013; Revised: July 11, 2013; Accepted: July 15, 2013; Published: June 1, 2014Show citation

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Hong, M.J., Kim, D.Y., & Seo, Y.W. (2014). Cullin, a component of the SCF complex, interacts with TaRMD5 during wheat spike development. Biologia plantarum, 58(2), 218-230. doi: 10.1007/s10535-013-0383-4.
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