Biologia plantarum 2010, 54:607-613 | DOI: 10.1007/s10535-010-0110-3

Effect of abscisic acid on heat stress tolerance in the calli from two ecotypes of Phragmites communis

W. Ding1, L. Song1, X. Wang1, Y. Bi1,*
1 School of Life Sciences, Lanzhou University, Lanzhou, P.R. China

Dune reed (DR) and swamp reed (SR) are two ecotypes of reed (Phragmites communis Trin.) that displayed differences in stress tolerance. To uncover the molecular basis for such difference, the effects of heat stress were studied using the calli derived from the two ecotypes. Heat stress caused increased ion leakage, inhibited growth, decreased cell viability, and elevated hydrogen peroxide (H2O2) and malondialdehyde (MDA) contents in the calli of both ecotypes, but DR callus showed better heat tolerance than SR callus. In DR callus, heat stress caused significant increase in the endogenous ABA content but not in SR callus. Application of fluridone (an ABA synthesis inhibitor) aggravated the heat stress damages on the DR callus whereas it had only minimal impact on the SR callus. Exogenous application of ABA alleviated the heat stress symptoms in the calli of both ecotypes. ABA treatment increased the activities of superoxide dismutase, catalase, ascorbate peroxidase and peroxidase, and also decreased H2O2 and MDA contents. These results indicate that the ability of ABA synthesis under heat stress is a key factor attributing to the higher heat tolerance of DR than SR.

Keywords: antioxidant enzymes; dune reed; fluridone; hydrogen peroxide; lipid peroxidation; lipoxygenase; proline; swamp reed; thiol
Subjects: abscisic acid (ABA); ascorbate peroxidase; catalase; dune reed; electrolyte leakage; hydrogen peroxide; in vitro culture, callus; lipoxygenase; malondialdehyde; peroxidase; Phragmites communis; proline; relative water content (RWC); superoxide dismutase (SOD); swamp reed; temperature high

Received: April 29, 2009; Accepted: September 23, 2009; Published: December 1, 2010Show citation

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Ding, W., Song, L., Wang, X., & Bi, Y. (2010). Effect of abscisic acid on heat stress tolerance in the calli from two ecotypes of Phragmites communis. Biologia plantarum, 54(4), 607-613. doi: 10.1007/s10535-010-0110-3.
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