Biologia plantarum 2010, 54:711-714 | DOI: 10.1007/s10535-010-0126-8

Production of transgenic Pinus armandii plants harbouring btCryIII(A) gene

X. Z. Liu1, Z. Liu2, Y. M. Yang1, H. Y. Zhang1,3,*
1 White Dragon Temple, Southwest Forestry University, Kunming, Yunnan Province, P.R. China
2 Department of Chemistry and Biochemistry, Brigham Young University, Provo, USA
3 School of Biological Sciences, University of Auckland, Auckland, New Zealand

A synthetic chimeric gene SbtCryIII(A) encoding the insecticidal protein btCryIII(A), was transformed into Pinus armandii embryos and embryogenic calli using Agrobacterium tumefaciens. Polymerase chain reaction and genomic DNA Southern blot analysis showed that the SbtCryIII(A) gene was integrated into the genome of transgenic Pinus armandii plants, and Northern blot analysis indicated that the SbtCryIII(A) gene was transcribed.

Keywords: embryo; gene for insect resistance; Northern blot; PCR; Southern blot
Subjects: pine

Received: December 20, 2008; Accepted: September 10, 2009; Published: December 1, 2010Show citation

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Liu, X.Z., Liu, Z., Yang, Y.M., & Zhang, H.Y. (2010). Production of transgenic Pinus armandii plants harbouring btCryIII(A) gene. Biologia plantarum, 54(4), 711-714. doi: 10.1007/s10535-010-0126-8.
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