Biologia plantarum 2011, 55:396-399 | DOI: 10.1007/s10535-011-0063-1

Isolation and characterization of eleven polymorphic microsatellite loci in Aegiphila sellowiana and their transferability

E. A. Ruas1, J. O. Damasceno1, A. R. O. Conson2, B. F. Costa2, L. A. Rodrigues2, M. Reck2, A. O. Santos Vieira3, C. deF. Ruas2, C. Medri4, P. M. Ruas2,*
1 Departamento de Agronomia, Centro de Ciências Agrárias, Universidade Estadual de Londrina, Londrina, Paraná, Brazil
2 Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, Paraná, Brazil
3 Departamento de Biología Animal e Vegetal, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, Paraná, Brazil
4 Departamento de Biologia, Universidade do Norte do Paraná, Bandeirantes, Paraná, Brazil

We isolated and characterized eleven polymorphic microsatellite loci for Aegiphila sellowiana an outcrossing pioneer tree species that is frequently used in reforestation programs of tropical riparian forests in Brazil. A total of 38 alleles were detected across a sample of 45 individuals of A. sellowiana, with an average number of 3.45 alleles per locus. The average polymorphic information content (PIC) was 0.430 and the observed (HO) and expected (HE) heterozygosity values varied from 0.156 to 1.000 and 0.145 to 0.730, respectively. Eight loci exhibited significant deviation from Hardy-Weinberg equilibrium (P ≤ 0.001) and 32 pair combinations of loci showed significant linkage disequilibrium (P ≤ 0.001). All 11 primers were tested for cross amplification in 12 species belonging to the family Lamiaceae and 5 species belonging to the related family Verbenaceae. The sequence and diversity information obtained using these microsatellites and their cross-transferability to other species of Lamiaceae as well as Verbenaceae will increase our understanding of genetic structures and species relationships within Aegiphyla and other genera of these families.

Keywords: cross amplification; genetic diversity; Lamiaceae; microsatellite primers; Verbenaceae

Received: January 8, 2010; Accepted: June 29, 2010; Published: June 1, 2011Show citation

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Ruas, E.A., Damasceno, J.O., Conson, A.R.O., Costa, B.F., Rodrigues, L.A., Reck, M., ... Ruas, P.M. (2011). Isolation and characterization of eleven polymorphic microsatellite loci in Aegiphila sellowiana and their transferability. Biologia plantarum, 55(2), 396-399. doi: 10.1007/s10535-011-0063-1.
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