Biologia plantarum 43:381-386, 2000 | DOI: 10.1023/A:1026730008917

Induction of Oxidative Stress and Antioxidant Activity by Hydrogen Peroxide Treatment in Tolerant and Susceptible Wheat Genotypes

R.K. Sairam1,*, G.C. Srivastava1
1 Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi -, India e-mail

We induced an oxidative stress by means of exogenous hydrogen peroxide in two wheat genotypes, C 306 (tolerant to water stress) and Hira (susceptible to water stress), and investigated oxidative injury and changes in antioxidant enzymes activity. H2O2 treatment caused chlorophyll degradation, lipid peroxidation, decreased membrane stability and activity of nitrate reductase. Hydrogen peroxide increased the activity of antioxidant enzymes, glutathione reductase and catalase. These effects increased with increasing H2O2 concentrations. However, no change was observed in the activity of superoxide dismutase and proline accumulation.

Keywords: active oxygen species; catalase; chloroplast; glutathione reductase; lipid peroxidation; nitrate reductase; proline; superoxide dismutase; Triticum aestivum
Subjects: antioxidant activity, oxidative stress, induction by H2O2; catalase, tolerance to water stress; chloroplast, tolerance to water stress; glutathione reductase, tolerance to water stress; lipid peroxidation, tolerance to water stress; nitrate reductase, tolerance to water stress; oxidative stress, antioxidant activity, induction by H2O2; oxygen species, tolerance to water stress; proline, tolerance to water stress; superoxide dismutase, tolerance to water stress; Triticum aestivum; water stress, tolerance, wheat genotypes; wheat genotypes, tolerance to water stress

Published: September 1, 2000Show citation

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Sairam, R.K., & Srivastava, G.C. (2000). Induction of Oxidative Stress and Antioxidant Activity by Hydrogen Peroxide Treatment in Tolerant and Susceptible Wheat Genotypes. Biologia plantarum, 43(3), 381-386. doi: 10.1023/A:1026730008917.
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