Biologia plantarum 44:181-184, 2001 | DOI: 10.1023/A:1010278704613

Effect of Sucrose Concentration, Charcoal, and Indole-3-Butyric Acid on Germination of Abies Numidica Somatic Embryos

B. Vooková1, A. Kormuťák1
1 Institute of Plant Genetics and Biotechnology, Slovak Academy of Science, Nitra, Slovak Republic

The dependency of radicle elongation in Abies numidica somatic embryos on germination media has been studied. No significant differences were detected between the Murashige and Skoog (MS) and Schenk and Hildebrandt (SH) medium. The addition of 10 g dm-3 activated charcoal or 0.05 mg dm-3 indole-3-butyric acid (IBA) into both media had positive influence on embryo germination. Difference between activated charcoal and IBA effects were significant. The high rooting percentage (85 %) was recorded on half SH medium with 10 g dm-3 sucrose and activated charcoal. After IBA addition rooting percentage was increased to 95 %. During 7 months 73 % of plantlets survived transfer to soil and in 54 % of plantlets shoot growth was observed.

Keywords: Algerian fir; conifers; root formation
Subjects: Abies numidica; auxins; fir, rhizogenesis in vitro, somatic embryos; in vitro culture, root formation

Published: June 1, 2001Show citation

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Vooková, B., & Kormuťák, A. (2001). Effect of Sucrose Concentration, Charcoal, and Indole-3-Butyric Acid on Germination of Abies Numidica Somatic Embryos. Biologia plantarum, 44(2), 181-184. doi: 10.1023/A:1010278704613.
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