Biologia plantarum 46:285-288, 2003 | DOI: 10.1023/B:BIOP.0000022267.04735.66

The Fructose Transport Mechanism in Microsomal Membrane Vesicles from Rye Roots

M. Kasai1, Y. Iwamatsu1, H. Hayashi1, S. Sawada1
1 Department of Biofunctional Science, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Japan

In a fructose-containing medium in which rye root-microsomal membrane vesicles had reached the equilibrium of uptake of fructose, the presence of both Mg2+ and ATP caused the efflux of fructose from the vesicles. Among nucleotides examined, ATP caused the largest efflux of fructose. The efflux of fructose dependent on Mg2+ and ATP was quite insensitive to a protonophore, carbonylcyanide m-chlorophenylhydrazone (CCCP), which actually abolished MgATP-dependent proton accumulation in the vesicles, while it was largely inhibited by vanadate, which inhibits ATP-binding cassette transporters (ABCTs). The Michaelis-Menten constant (Km) of the efflux of fructose was 0.4 mM. It was observed that fructose stimulated the ATPase activity of the vesicles and that vanadate markedly decreased the fructose-stimulated ATPase activity.

Keywords: ATP-binding cassette transporter; MgATP; ΔpH; Secale cereale
Subjects: ATP-binding cassette transporter; fructose transport mechanism; microsomal membrane vesicles; rye, fructose transport mechanism; saccharide transport mechanism; Secale cereale

Published: September 1, 2003Show citation

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Kasai, M., Iwamatsu, Y., Hayashi, H., & Sawada, S. (2003). The Fructose Transport Mechanism in Microsomal Membrane Vesicles from Rye Roots. Biologia plantarum, 46(2), 285-288. doi: 10.1023/B:BIOP.0000022267.04735.66.
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    References

    1. Boorer, K.J., Loo, D.D.F., Wright, E.M.: Steady-state and presteady-state kinetics of the H+/hexose cotransporter (STP1) from Arabidopsis thaliana expressed in Xenopus oocytes.-J. biol. Chem. 269: 20417-20424, 1994.
    2. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
    3. Buckhout, T.J., Tubbe, A.: Structure, mechanisms of catalysis, and regulation of sugar transporters in plants.-In: Zamski, E., Schaffer, A.A. (ed.): Photoassimilate Distribution in Plants and Crops. Pp. 229-260. Marcel Dekker, New York 1996.
    4. Büttner, M., Sauer, N.: Monosaccharide transporters in plants: structure, function and physiology.-Biochim. biophys. Acta 1465: 263-274, 2000. Go to original source...
    5. Heinonen, J.K., Lahti, R.J.: A new and convenient colorimetric determination of inorganic orthophosphate and its application to the assay of inorganic pyrophosphatase.-Anal. Biochem. 113: 313-317, 1981. Go to original source...
    6. Kasai, M., Nakamura, T., Kudo, N., Sato, H., Maeshima, M., Sawada, S.: The activity of the root vacuolar H+-pyrophosphatase in rye plants grown under conditions deficient in mineral nutrients.-Plant Cell Physiol. 39: 890-894, 1998. Go to original source...
    7. Lambert, A., Osteras, M., Mandon, K., Poggi, M.C., Le Rudulier, D.: Fructose uptake in Sinorhizobium meliloti is mediated by a high-affinity ATP-binding cassette transport system.-J. Bacteriol. 183: 4709-4717, 2001. Go to original source...
    8. Martinoia, E., Klein, M., Geisler, M., Bovet, L., Forestier, C., Kolukisaoglu, Ü., Müller-Röber, B., Schulz, B.: Multifunctionality of plant ABC transporters - -more than just detoxifiers.-Planta 214: 345-355, 2002. Go to original source...
    9. Milner, I.D., Ho, L.C., Hall, J.L.: Properties of proton and sugar transport at the tonoplast of tomato (Lycopersicon esculentum) fruit.-Physiol. Plant. 94: 399-410, 1995. Go to original source...
    10. Shiratake, K., Kanayama, Y., Yamaki, S.: Characterization of hexose transporter for facilitated diffusion of the tonoplast vesicles from pear fruit.-Plant Cell Physiol. 38: 910-916, 1997. Go to original source...
    11. Stanzel, M., Sjolund, R.D., Komor, E.: Transport of glucose, fructose and sucrose by Streptanthus tortuosus suspension cells.-Planta 174: 201-209, 1988. Go to original source...
    12. Stitt, M., Lilley, R.Mc.C., Gerhardt, R., Heldt, H.W.: Determination of metabolite levels in specific cells and subcellular compartments of plant leaves.-Methods Enzymol. 174: 518-552, 1989. Go to original source...
    13. Sturm, A., Tang, G.-Q.: The sucrose-cleaving enzymes of plants are crucial for development, growth and carbon partitioning.-Trends Plant Sci. 4: 401-407, 1999. Go to original source...
    14. Sze, H.: H+-translocating ATPases: advances using membrane vesicles.-Annu. Rev. Plant Physiol. 36: 175-208, 1985. Go to original source...

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