Biologia plantarum 48:431-436, 2004 | DOI: 10.1023/B:BIOP.0000041098.18836.be

Introduction of Resistance to Herbicide Basta® in Savoy Cabbage

T. Sretenović-Rajičić1, S. Ninković2, B. Vinterhalter2, J. Miljuš-Djukić3, D. Vinterhalter4
1 Centre for Vegetable Crops, Smederevska Palanka, Serbia and Montenegro, India
2 Institute for Biological Research "S. Stanković", 29, Belgrade, Serbia and Montenegro, India e-mail
3 Institute of Molecular Genetics and Genetic Engineering, Belgrade, Serbia and Montenegro, India
4 Faculty of Science, Sarajevo, Bosnia and Herzegovina, India

Resistance to herbicide Basta® was introduced into pure inbred lines of Savoy cabbage (Brassica oleracea L. var. sabauda) by cocultivation of cotyledon and hypocotyl explants with Agrobacterium tumefaciens strains AGL1/pDM805 and LBA4404/pGKB5 (LB5-1). Shoot regeneration occurred on Murashige and Skoog medium supplemented with 1 mg dm-3 6-benzyladenine and 0.5 mg dm-3 indole-3-butyric acid at 42.3 % and 71.4 % of hypocotyl explants treated with AGL1/pDM805 and LB5-1, respectively. Putative transformants that survived selection on 10 mg dm-3 phosphinothricin (L-PPT) supplemented medium were confirmed by GUS assay and PCR analysis. The transformation rate was 58 % with AGL1/ pDM805 and 25 % with LB5-1. Rooted plantlets were acclimated and then again screened for Basta®-resistance by spraying with 15 - 60 mg dm-3 L-PPT. Surviving plants were selfed and Basta®-resistance was demonstrated in T1 progeny.

Keywords: Agrobacterium; bar gene; Brassica oleracea var. sabauda; transformation
Subjects: Agrobacterium-mediated transformation; Agrobacterium tumefaciens; auxins; Brassica oleracea var. sabauda; cytokinins; herbicide, resistance induced to; nutrient medium, Murashige and Skoog; polymerase chain reaction (PCR); resistance to herbicide Basta®, Savoy cabbage; Savoy cabbage, resistance to herbicide; transformation, Agrobacterium-mediated; transgenic plants

Published: September 1, 2004Show citation

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Sretenović-Rajičić, T., Ninković, S., Vinterhalter, B., Miljuš-Djukić, J., & Vinterhalter, D. (2004). Introduction of Resistance to Herbicide Basta® in Savoy Cabbage. Biologia plantarum, 48(3), 431-436. doi: 10.1023/B:BIOP.0000041098.18836.be.
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