Biologia plantarum 49:129-132, 2005 | DOI: 10.1007/s10535-005-0132-4

In vitro regeneration of Perilla frutescens from hypocotyl and cotyledon explants

S. W. Hou1,*, J. F. Jia2
1 School of Life Science, Lanzhou University, Lanzhou, P.R. China
2 School of Life Science, Northwest University, Xi'an, P.R. China

Organogenetic buds were induced from hypocotyl and cotyledon explants of oil crop Perilla frutescens in Murashige and Skoog (MS) medium supplemented with 5.7 μM indole-3-acetic acid (IAA) and 8.9 - 13.3 μM 6-benzylaminopurine (BA). Shoots were rooted on MS medium with 2.9 μM IAA and 1.4 μM gibberellic acid (GA3) and the regenerated plants flowered and set seeds normally.

Keywords: growth regulators; organogenesis
Subjects: auxins; cotyledons; cytokinins; gibberellic acid, gibberellins; hypocotyl; in vitro culture, from cotyledon; in vitro culture, from hypocotyl; in vitro culture, regeneration, organogenesis, morphogenesis; medicinal plants; nutrient medium MS (Murashige and Skoog); Perilla frutescens

Received: September 9, 2003; Accepted: August 10, 2004; Published: March 1, 2005Show citation

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Hou, S.W., & Jia, J.F. (2005). In vitro regeneration of Perilla frutescens from hypocotyl and cotyledon explants. Biologia plantarum, 49(1), 129-132. doi: 10.1007/s10535-005-0132-4.
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    References

    1. Chen, Y., Kenaschuk, E., Dribnenki, P.: High frequency of plant regeneration from anther culture in flax (Linum usitatissimum L.).-Plant Breed. 117: 463-467, 1998. Go to original source...
    2. Eapen, S., George, L.: Plant regeneration from peduncle segments of oil seed Brassica species: influence of silver nitrate and silver thiosulfate.-Plant Cell Tissue Organ Cult. 51: 229-232, 1997. Go to original source...
    3. Guo, B.Z., Wu, Z.L., Lu, S.L., Liu, S.W., Yang, Y.C., He, T.N., Huang, R.F. (ed.): Flora of Qinghai. Economic Plants, Section II.-Qinghai People 's Press, Xining 1987.
    4. Liu, X.Q., Wan, F.Z., Zhen, S.L.: [Effects of anti-bacteria and fungus of Perilla frutescens.]-Natur. Product Res. Develop. 12: 42-45, 2000. [In Chin.]
    5. Liu, Y.X., Zhang, W.M., Qian, X.S.: [Research and utilization of Perilla.]-Wild Plant Resour. China 3: 24-27, 1996. [In Chin.]
    6. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant 15: 473-497, 1962. Go to original source...
    7. Yao, D.D., Yu, H.H.: [On the theory of developing Perilla frutescens.]-J. Jilin Forest. Univ. 15: 25-28, 1999. [In Chin.]

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