Biologia plantarum 49:273-276, 2005 | DOI: 10.1007/s10535-005-3276-3

Alleviation of salt stress by low dose γ-irradiation in rice

M.-H. Baek1, J.-H. Kim1, B. Y. Chung1,*, J.-S. Kim1, I. S. Lee1
1 Division of Radiation Application Research, Korea Atomic Energy Research Institute, Yuseong-gu, Daejon, Republic of Korea

The effects of salt stress on the growth, photosynthesis, and antioxidative ability of the rice (Oryza sativa L.) plants raising from γ-irradiated seeds were investigated using two cultivars, Ilpumbyeo and Sanghaehyanghyella. The 50 and 100 mM NaCl solutions caused a remarkable decrease of the early germination rate and seedling growth. However, the salt stress-induced inhibition of the growth was significantly alleviated in the γ -irradiated plants. The chlorophyll contents and the effective quantum yield of photosystem 2 (Φ PS 2) were lower in the NaCl-treated plants than in the control ones, while the non-photochemical quenching was higher in the former ones. Activities of the antioxidant enzymes such as superoxide dismutase (SOD) and ascorbate peroxidase (APX) increased with increasing NaCl concentrations, and the irradiated groups had even higher SOD and APX activities than the non-irradiated ones. These alleviation effects were observed similarly in both the cultivars tested.

Keywords: ascorbate peroxidase; fluorescence parameters; NaCl; Oryza sativa; photosynthesis; superoxide dismutase
Subjects: ascorbate peroxidase; chlorophyll fluorescence, salt stress, gamma-radiation; cultivar and genotype differences, γ-radiation; gamma-radiation, salt stress alleviation; germination; Oryza sativa; photosystems 1 and 2; rice, salt stress alleviation, gamma-radiation; salt stress, alleviation, gamma-radiation; stress alleviation; superoxide dismutase

Received: February 25, 2004; Accepted: November 30, 2004; Published: June 1, 2005Show citation

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Baek, M.-H., Kim, J.-H., Chung, B.Y., Kim, J.-S., & Lee, I.S. (2005). Alleviation of salt stress by low dose γ-irradiation in rice. Biologia plantarum, 49(2), 273-276. doi: 10.1007/s10535-005-3276-3.
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    References

    1. Ahmed, I.V., Faiz, S.M.A., Anwar Hussain, A.K.M., Setter, A.: Seed germination and early seedling growth of rice under salt stress as means of sorting out salt tolerant genotype.-Curr. Agr. 11: 35-40, 1987.
    2. Allen, R.: Dissection of oxidative stress tolerance using transgenic plants.-Plant Physiol. 107: 1049-1054, 1995. Go to original source...
    3. Arnon, D. I.: Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris.-Plant Physiol. 24: 1-14, 1949. Go to original source...
    4. Asada, K.: The water-water cycle in chloroplasts: scavenging of active oxygen and dissipation of excess photons.-Annu. Rev. Plant Physiol. Plant mol. Biol. 50: 601-639, 1999. Go to original source...
    5. Beyer, W.F., Fridovich, I.: Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions.-Anal. Biochem. 161: 559-566, 1987. Go to original source...
    6. Bradford, M.N.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
    7. Delfine, S., Alvino, A., Villani, M.C., Loreto, R.: Restrictions to carbon dioxide conductance and photosynthesis in spinach leaves recovering from salt stress.-Plant Physiol. 119: 1101-1106, 1999. Go to original source...
    8. Demmig-Adams, B., Adams, W.W., III: The role of the xanthophylls cycle carotenoids in the protection of photosynthesis.-Trends Plant Sci. 1: 21-26, 1996. Go to original source...
    9. Fridovich, I.: Superoxide dismutases.-Progr. nucleic Acid Res. 40: 220-253, 1991.
    10. Genty, B., Briantais, J.M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence.-Biochim. biophys. Acta 90: 87-92, 1989. Go to original source...
    11. Halliwell, B.: The toxic effects of oxygen on plant tissues.-In: Oberley, L.W. (ed): Superoxide Dismutase. Vol. I. Pp. 89-123. CRC Press, Boca Raton 1982.
    12. Hernández, J.A., Olmos, E., Corpas, F.J., Sevilla, F., Del Rio, L.A.: Salt-induced oxidative stress in chloroplasts of pea plants.-Plant Sci. 105: 151-167, 1995. Go to original source...
    13. Kumar, S.G., Lakshmi, A., Madhusudhan, K.V., Ramanjulu, S., Sudhakar, C.: Photosynthesis parameters in two cultivars of mulberry differing in salt tolerance.-Photosynthetica 36: 611-616, 1999. Go to original source...
    14. Lee, H.Y., Kim, J.S., Baek, M.H., Park, S.C., Park, Y.I.: Effects of low dose γ-radiation on photosynthesis of red pepper (Capsicum annuum L.) and the reduction of photoinhibition.-Kor. J. environ. Agr. 21: 83-89, 2002.
    15. Lee, H.Y., Kim, J.S., Baek, M.H., Yoo, J.C., Kwon, S.T.: Effects of low dose γ-irradiation on the physiological activities of radish (Raphanus sativus L.) during early growth and the reduction of ultraviolet-B stress.-Kor. Soc. hort. Sci. 44: 314-320, 2003.
    16. Malanga, G., Calmanovici, G., Puntarulo, S.: Oxidative damage to chloroplasts from Chlorella vulgaris exposed to ultraviolet-B radiation.-Physiol. Plant. 101: 455-462. 1997. Go to original source...
    17. Meloni, D.A., Oliva, M.A., Martunez, C.A., Cambraia, J.: Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress.-Environ. exp. Bot. 49: 69-76, 2003. Go to original source...
    18. Nakano, Y., Asada, K.: Hydrogen peroxide is scavenged by ascorbate peroxidase in spinach chloroplasts.-Plant Cell Physiol. 22: 867-880, 1981.
    19. Núñez, M., Mazzafera, P., Mazorra, L. M., Siqueira, W. J., Zullo, M. A. T.: Influence of a brassinosteroid analogue on antioxidant enzymes in rice grown in culture medium with NaCl.-Biol. Plant. 47: 67-70, 2003/4.
    20. Özmen, A.D., Özdemir, F., Türkan, I.: Effects of paclobutrazol on response of two barely cultivars to salt stress.-Biol. Plant. 46: 263-268, 2003.
    21. Prisco, J.T., O'Leary, J.W.: Osmotic and toxic effects of salinity on germination of Phaseolus vulgaris L. seeds.-Turrialba 20: 177-184, 1970.
    22. Promila, K., Kumar, S.: Vigna radiata seed germination under salinity.-Biol. Plant. 43: 423-426, 2000. Go to original source...
    23. Rout, N.P., Shaw, B.P.: Salt tolerance in aquatic macrophytes: possible involvement of the antioxidative enzymes.-Plant Sci. 160: 412-423, 2001. Go to original source...
    24. Sultana, N., Ikeda, T. Itoh, R.: Effects of NaCl salinity on photosynthesis and dry matter accumulation in developing rice grain.-Environ. exp. Bot. 42: 211-220, 1999. Go to original source...
    25. Vaidyanathan, H., Sivakumar, P., Chakrabarty, R., Thomas, G.: Scavenging of reactive oxygen species in NaCl-stressed rice (Oryza sativa L.) - differential response in salt-tolerant and sensitive varieties.-Plant Sci. 165: 1411-1418, 2003. Go to original source...
    26. Van Kooten, O., Snel, F.H.: The use of chlorophyll fluorescence nomenclature in plant stress physiology.-Photosynth. Res. 25: 147-150, 1990. Go to original source...
    27. Zaka, R, Vandecasteele, C.M., Misset, M.T.: Effects of low chronic doses of ionizing radiation on antioxidant enzymes and G6PDH activities in Stipa capillata (Poaceae).-J. exp. Bot. 53: 1979-1987, 2002.

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