Biologia plantarum 49:481-485, 2005 | DOI: 10.1007/s10535-005-0035-4

Sugar cane buds as an efficient explant for plantlet regeneration

D. E. Vazquez Molina1, A. De Los Santos1, K. A. Lecona Guzman1, O. Sumano Muniz1, M. Velazquez Mendez1, R. Rincon Rosales1, M. A. Oliva Llaven2, L. Dendooven3,*, F. A. Gutierrez-Miceli1
1 Laboratory of Vegetal Biotechnology, Instituto Tecnologico de Tuxtla-Gutierrez, Tuxtla Gutierrez, Chiapas, Mexico
2 Facultad de Medicina Veterinaria y Zootecnia, Universidad Autonoma de Chiapas, Chiapas, Mexico
3 Departamento de Biotecnologia y Bioingenieria, CINVESTAV-IPN, Mexico DF, Mexico

An efficient and reproducible protocol for regeneration of plantlets at a high frequency was developed by using sugar cane buds. Disinfected buds were firstly submerged in ethanol sodium hypochlorite solution with 0.1 % polyvinylpyrrolidone, 1.5 % ascorbic acid and 1.75 % citric acid as antioxidants and subsequently treated with solution of agrimicin:captan (1:1). The upper stalk segment was better to obtain bud in vitro culture compared to lower segments. The medium for induction of multiple shoots consisted of Murashige and Skoog basal medium (MS) supplemented with 2 mg dm-3 thidiazuron and 1 mg dm-3 naphthalene acetic acid. An average of 24 shoots per bud was obtained for cv. Mex 68-P23 within four weeks and 29 shoots for cv. MY 55-14 within six weeks. Indole-3-butyric acid induced more roots in both cultivars compared to the untreated plantlets. Plantlets transferred to soil showed normal growth with up to four axilliary buds in each node. It was concluded that the germplasm obtained through the above mentioned technique generated stalks with more buds in each node which would give farmers more vegetative material for plantations in field with 100 % germination.

Keywords: bud culture; indole butyric acid; naphthalene acetic acid; Saccharum spp.; thidiazuron
Subjects: auxins; cytokinins; humic substances; in vitro culture, axillary buds proliferation, bud breaking and regeneration; nutrient medium MS (Murashige and Skoog); polyvinylpyrrolidone; sugar cane, regeneration, sucrose accumulation; thidiazuron (TDZ)

Received: June 21, 2004; Accepted: May 5, 2005; Published: December 1, 2005Show citation

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Molina, D.E., De Los Santos, A., Guzman, K.A., Muniz, O., Mendez, M., Rosales, R., ... Gutierrez-Miceli, F.A. (2005). Sugar cane buds as an efficient explant for plantlet regeneration. Biologia plantarum, 49(4), 481-485. doi: 10.1007/s10535-005-0035-4.
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    References

    1. Abreu, I.N., Azevedo, M.T.A., Solferini, V.M., Mazzafera, P.: In vitro propagation and isozyme polymorphism of the medicinal plant Hypericum brasiliense.-Biol. Plant. 47: 629-632, 2003/04.
    2. Amutha, S., Ganapathi, A., Muruganantham, M.: In vitro organogenesis and plant formation in Vigna radiata (L.) Wilczek.-Plant Cell Tissue Organ Cult. 72: 203-207, 2003. Go to original source...
    3. Bacchetta, L., Remotti, P.C., Bernardini, C., Saccardo, F.: Adventitious shoot regeneration from leaf explants and stem nodes of Lilium.-Plant Cell Tissue Organ Cult. 74: 37-44, 2003. Go to original source...
    4. Bedir, E., Lata, H., Schaneberg, B., Khan, I.A., Moraes, R.M.: Micropropagation of Hydrastis canadensis Goldenseal: a North American endangered species.-Planta med. 69: 86-88, 2003. Go to original source...
    5. Calic, D., Zdravkovic-Korac S., Pemac, D., Radojevic, Lj.: Variability and bimodal distribution of size in microspores of Aesculus hippocastanum.-Biol. Plant. 47: 457-458, 2003/04.
    6. Capelle, S.C., Mok, D.W.S., Kirchner, S.C., Mok, M.C.: Effect of thidiazuron on cytokinin autonomy and the metabolism of N6-(2-isopoentenyl) [8-14C] adenosine in callus tissue of Phaseolus lunatus.-Plant Physiol. 73: 796-802, 1983. Go to original source...
    7. Chang, H.S., Chakrabarty, D., Hahn, E.J., Paek, K.Y.: Micropropagation of calla lily (Zantedeschia albomaculata) via in vitro shoot tip proliferation.-In Vitro cell dev. Biol. Plant. 39: 129-134, 2003. Go to original source...
    8. Cui, M.L., Ezura, H.: Agrobacterium-mediated transformation of Nemesia strumosa Benth, a model plant for asymmetric floral development.-Plant Sci. 165: 863-870, 2003. Go to original source...
    9. Ganeshan, S., Baga, M., Harvey, B.L., Rossnagel, B.G., Scoles, G.J., Chibbar, R.N.: Production of multiple shoots from thidiazuron-treated mature embryos and leaf-base/apical meristems of barley (Hordeum vulgare).-Plant Cell Tissue Organ Cult. 73: 57-64, 2003. Go to original source...
    10. Gutierrez-Miceli, F.A., Morales-Torres, R., Espinosa-Castaneda, Y. de J., Rincon-Rosales, R., Montes-Molina, J., Oliva-Llaven, M.A., Dendooven, L.: Effects of partial defoliation on sucrose accumulation, enzyme activity and agronomic parameters in sugar cane (Saccharum spp.).-J. Agron. Crop Sci. 190: 256-261, 2004. Go to original source...
    11. Hiregoudar, L.V., Murthy, H.N., Hema, B.P., Hahn, E.J., Paek, K.Y.: Multiple shoot induction and plant regeneration of Feronia limonia L. Swingle.-Scientia Hort. 98: 357-364, 2003. Go to original source...
    12. Jaime-Gomez, A.: Micropropagacion de cana de azucar (Saccharum officinarum L) a partir de meristemos apicales y yemas laterales.-Thesis. Universidad Autonoma del Estado de Morelos, Mexico 1994.
    13. Kapchina-Toteva, V., Stoyanova, D.: Effect of cytokinins and cytokinin antagonists on in vitro cultured Gypsophila paniculata L.-Biol. Plant. 46: 337-341, 2003. Go to original source...
    14. Laloue, M., Fox, J.E.: Cytokinin oxidase from wheat.-Plant Physiol. 90: 899-906, 1989. Go to original source...
    15. Lantcheva, A.M., Vlahova, E., Bakalova, E., Kondorosi, M.C., Elliott, A., Atanassov, V.: Regeneration of diploid annual medics via direct somatic embryogenesis promoted by thidiazuron and benzylaminopourine.-Plant Cell Rep. 18: 904-910, 1999. Go to original source...
    16. Lata, H., Bedir, E., Hosick, A., Ganzera, M., Khan, I., Moraes, R.M.: In vitro plant regeneration from leaf-derived callus of Cimicifuga racemosa.-Planta med. 68: 912-915, 2002. Go to original source...
    17. Li, W., Gao, H.H., Lu, R., Guo, G.Q., Zheng, G.C.: Direct plantlet regeneration from the tuber of Stachys sieboldii.-Plant Cell Tissue Organ Cult. 71: 259-262, 2002. Go to original source...
    18. Malik, K.A., Saxena, P.K.: Regeneration in Phaseolus vulgaris L. High frequency induction of direct shoot formation in intact seedlings by N6-benzylaminopurine and thidiazuron.-Planta 186: 384-389, 1992. Go to original source...
    19. Martin, K.P., Beena, M.R., Joseph, D.: High frequency axillary bud multiplication and ex vitro rooting of Wedelia chinensis (Osbeck) Merr. a medicinal plant.-Indian J. exp. Biol. 41: 262-266, 2003.
    20. Masciandaro, G., Cecanti, B., Ronchi, V., Benedicto, S., Howard, L.: Humic substances to reduce salt effect on plant germination and growth.-Commun. Soil Sci. Plant Anal. 33: 365-378, 2002. Go to original source...
    21. Mithila, J., Hall, J.C., Victor, J.M.R., Saxena, P.K.: Thidiazuron induces shoot organogenesis at low concentrations and somatic embryogenesis at high concentrations on leaf and petiole explants of African violet (Saintpaulia ionantha Wendl.).-Plant Cell Rep. 21: 408-414, 2003. Go to original source...
    22. Mok, M.C., Mok, D.W.S., Armstrong, D.J., Shudo, K., Isogai, Y., Okamoto, T.: Cytokinin activity of N-phenyl-N-1,2,3-thiadiazol-5-ylurea (thidiazuron).-Phytochemistry 21: 1509-1511, 1982. Go to original source...
    23. Moutla, M., Dookum, A.: Evaluation of surface sterilization and hot water treatments on bacterial contaminants in bud culture of sugar cane.-Exp. Agr. 35: 265-74, 1999.
    24. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue culture.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    25. Qu, L., Chen, J., Henny, R.J., Huang, Y., Caldwell, R.D., Robinson, C.A.: Thidiazuron promotes adventitious shoot regeneration from pothos (Epipremnum aureum) leaf and petiole explants.-In Vitro cell dev. Biol. Plant. 38: 268-271, 2002. Go to original source...
    26. Saito, H., Nakano, M.: Plant regeneration from suspension cultures of Hosta sieboldiana.-Plant Cell Tissue Organ Cult. 71: 23-28, 2002. Go to original source...
    27. Saxena, P.K., Malik, K.A., Gill, R.: Induction by thidiazuron of somatic embryogenesis in intact seedlings of peanut.-Planta 17: 421-424, 1992. Go to original source...
    28. Shyamkumar, B., Anjaneyulu, C., Giri, C.C.: Multiple shoot induction from cotyledonary node explants of Terminalia chebula.-Biol. Plant. 47: 585-588, 2003/04.
    29. Singh, N.D., Sahoo, L., Sarin, N.B., Jaiwal, P.K.: The effect of TDZ on organogenesis and somatic embryogenesis in pigeonpea (Cajanus cajan L. Millsp).-Plant Sci. 164: 341-347, 2003. Go to original source...
    30. Thimmappaiah, S., Sadhana, R.A.: In vitro propagation of cashew from young trees.-In Vitro cell dev. Biol. Plant 38: 152-156, 2002. Go to original source...
    31. Thomas, J.C., Katterman, F.R.: Cytokinin activity induced by thidiazuron.-Plant Physiol. 81: 681-683, 1986. Go to original source...
    32. Zacchini, M., De Agazio, M.: Micropropagation of a local olive cultivar for germplasm preservation.-Biol. Plant. 48: 589-592, 2004. Go to original source...
    33. Zhao, D.L., Guo, G.Q., Wang, X.Y., Zheng, G.C.: In vitro micropropagation of a medicinal plant species Sophora flavescens.-Biol. Plant. 47: 117-120, 2003/04.

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