Biologia plantarum 50:395-399, 2006 | DOI: 10.1007/s10535-006-0055-8

Antioxidant enzyme induction in pea plants under high irradiance

J. A. Hernandez1,*, C. Escobar2, G. Creissen3, P. M. Mullineaux4
1 Dept. de MejoraVegetal, Centro de Edafologia y Biologia Aplicada del Segura, CSIC, Espinardo-Murcia, Spain
2 Facultad de Ciencias del Medio Ambiente, Universidad de Castilla-La Mancha, Toledo, Spain
3 Dept. Disease and Stress Biology, John Innes Centre, Colney, Norwich, UK
4 Dept. Bioogical Science, University of Essex, Colchester, Essex, UK

Exposure of pea plants to high irradiance (HI) for 60 min caused a reversible photoinhibition as shown by changes in variable to maximum fluorescence ratio (Fv/Fm). A significant decline in Fm was observed in leaves from both pea cultivars subjected to HI, the decrease being higher in JI281 than in JI399 plants. The values recovered during the post-stress period in both cultivars. In both cultivars, minimal fluorescence (F0) increased under HI, but in cultivar JI399 F0 recovered to initial value during the post-stress period. The expression of antioxidant enzyme genes was higher in JI399 than in JI281, both in control and stressed plants. In JI281, after 60 min of HI, an induction of the transcripts of CAT, chlMDHAR, cytAPX and cytCu,Zn-SOD was observed, whereas there was a slight increase in PHGPX, stAPX and chlCu,Zn-SOD mRNAs. After 24 h of the recovery period, the induction of some transcripts was not maintained (CAT, cytAPX and cytCu,Zn-SOD), whereas the induction of others was maintained (PHGPX and chlCu,ZnSOD) or even increased (cytGR, stAPX and chlMDHAR). In JI399, CAT and cytAPX were increased strongly after 60 min of HI, and slight increases were observed in cytGR, chlGR and chlMDHAR. In the post-stress period the expression of stAPX, cytGR and chlMDHAR was even slightly higher than after 60 min of HI, however, expression of CAT, cytAPX, cytCu,ZnSOD, chlCu,ZnSOD and chlGR decreased.

Keywords: ascorbate peroxidase; catalase; gene expression; photooxidative stress; Pisum sativum; superoxide dismutase
Subjects: antioxidants, antioxidative enzymes; ascorbate peroxidase; catalase; chlorophyll fluorescence; glutathione reductase; Northern blot analysis; pea; photooxidative stress; Pisum sativum; superoxide dismutase (SOD)

Received: July 14, 2004; Accepted: May 20, 2005; Published: September 1, 2006Show citation

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Hernandez, J.A., Escobar, C., Creissen, G., & Mullineaux, P.M. (2006). Antioxidant enzyme induction in pea plants under high irradiance. Biologia plantarum, 50(3), 395-399. doi: 10.1007/s10535-006-0055-8.
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    References

    1. Anderson, J.M., Park, Y.I., Chow, W.S.: Photoinactivation and photoprotection of photosystem II in nature.-Physiol. Plant. 100: 214-223, 1997. Go to original source...
    2. Aro, E.M., McCaffery, S., Anderson, J.M.: Photoinhibition and D1 protein degradation in peas acclimated to different growth irradiances.-Plant Physiol. 103: 835-843, 1993. Go to original source...
    3. Balachandran, S., Osmond, C.B.: Susceptibility of tobacco leaves to photoinhibition following infection with two strains of tobacco mosaic virus under different light and nitrogen nutrition regimes.-Plant Physiol. 104: 1051-1057, 1994. Go to original source...
    4. Bertamini, M., Nedunchezhian, N.: Photosynthesis responses for Vitis vinifera plants grown at different photon flux densities under field conditions.-Biol. Plant. 48: 149-152, 2004. Go to original source...
    5. Bowler, C., Alliotte, T., De Loose, M., Van Montagu, M., Inze, D.: The induction of manganese superoxide dismutase in response to stress in Nicotiana plumbaginifolia.-EMBO J. 8: 31-38, 1989. Go to original source...
    6. Creissen, G.P., Mullineaux, P.M.: Cloning and characterisation of glutathione reductase cDNAs and identification of two genes encoding the tobacco enzyme.-Planta 197: 422-425, 1995. Go to original source...
    7. Dat, J., Van den Abeele, S., Vranova, E., Van Montagu, M., Inze, D., Van Breusegem, F.: Dual action of the active oxygen species during plant stress responses.-Cell. mol. Life Sci. 57: 779-795, 2000. Go to original source...
    8. Ellis, T.H.N., Turner, L., Hellens, R.P., Lee, D., Harker, C.L., Enard, C., Domoney, C., Davies, D.R.: Linkage maps in pea.-Genetics 130: 649-663, 1992.
    9. Foyer, C.H., Dujardyn, M., Lemoine, Y.: Responses of photosynthesis and the xanthophyll and ascorbate cycles to changes in irradiances, photoinhibition and recovery.-Plant Physiol. Biochem. 27: 751-760, 1989.
    10. Foyer, C.H., Halliwell, B.: The presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism.-Planta 133: 21-25, 1976. Go to original source...
    11. Gillham, D.J., Dodge, A.D. Chloroplasts superoxide and hydrogen peroxide scavenging systems from pea leaves: seasonal variations.-Plant Sci. 50: 105-109, 1987. Go to original source...
    12. Hernandez, J.A., Jimenez, A., Mullineaux, P.M., Sevilla, F.: Tolerance of pea (Pisum sativum L.) to long-term salt stress is associated with induction of antioxidant defences.-Plant Cell Environ. 23: 853-862, 2000. Go to original source...
    13. Hernandez, J.A., Escobar, C., Creissen, G., Mullineaux, P.M.: Role of hydrogen peroxide and the redox state of ascorbate in the induction of antioxidant enzymes in pea leaves under excess light stress.-Funct. Plant Biol. 31: 359-368, 2004. Go to original source...
    14. Ishikawa, T., Sakai, K., Takeda, T., Shigeoka, S.: Cloning and expression of cDNA encoding a new type of ascorbate peroxidase from spinach.-FEBS Lett. 367: 28-32, 1995. Go to original source...
    15. Karpinski, S., Escobar, C., Karpinska, B., Creissen, G., Mullineaux, P.M.: Photosynthetic electron transport regulates the expression of cytosolic ascorbate peroxidase genes in Arabidopsis during excess light stress.-Plant Cell 9: 627-640, 1997. Go to original source...
    16. Karpinski. S. Reynolds, H., Karpinska, B., Wingsle, G., Creissen, G., Mullineaux, P.M.: Systemic signaling and acclimation in response to excess excitation energy in Arabidopsis.-Science 284: 654-657, 1999. Go to original source...
    17. Krause, G.H., Weiss, E.: Chlorophyll fluorescence and photosynthesis.-Annu. Rev. Plant Physiol. Plant mol. Biol. 42: 313-349, 1991. Go to original source...
    18. Mishra, H.P., Fatma, T., Singhal, G.S.: Development of antioxidative defense system of wheat seedlings in response to high light.-Physiol. Plant. 95: 77-82, 1995. Go to original source...
    19. Mullineaux, P.M., Karpinski, S., Jimenez, A., Cleary, S., Robinson, C., Creissen, G.: Identification of cDNAs encoding plastid-targeted glutathione peroxidase.-Plant J. 13: 375-379, 1998. Go to original source...
    20. Murgia, I., Tarantino, D., Vannini, C., Bracale, M., Carravieri, S., Soave, C.: Arabidopsis thaliana plants overexpressing thylakoidal ascorbate peroxidase show increased resistance to paraquat-induced photooxidative stress and to nitric oxida-induced cell death.-Plant J. 38: 940-953, 2004. Go to original source...
    21. Prochazkova, D., Wilhelmova, N.: Changes in antioxidant protection in bean cotyledons during natural and continuous irradiation-accelerated senescence.-Biol. Plant. 48: 33-39, 2004. Go to original source...
    22. Santos, M., Gousseau, H., Lister, C., Foyer, C., Creissen, G., Mullineaux, P.M.: Cytosolic ascorbate peroxidase from Arabidopsis thaliana L. is encoded by a small multigene family.-Planta 198: 64-69, 1996. Go to original source...
    23. Stevens, R.G., Creissen, G.P., Mullineaux, P.M.: Cloning and characterization of a cytosolic glutathione reductase cDNA from pea (Pisum sativum L.) and its expression in response to stress.-Plant mol. Biol. 35: 641-654, 1997. Go to original source...
    24. Van Camp, W., Bowler, C., Villarroel, R., Tsang, E.W.T., Van Montagu, M., Inze, D.: Characterization of iron superoxide dismutase cDNAs from plants obtained by genetic complementation in Escherichia coli.-Proc. nat. Acad. Sci. USA 87: 9903-9907, 1990. Go to original source...
    25. Willekens, H., Langebartels, C., Tire, C., Van Montangu, M., Inze, D., Van Camp, W.: Differential expression of catalase genes in Nicotiana plumbaginifolia (L.).-Proc. nat. Acad. Sci. USA 91: 10450-10454, 1994. Go to original source...
    26. Yoshimura, K., Yabuta, Y., Ishikawa, T., Shigeoka, S.: Expression of spinach ascorbate peroxidase isoenzymes in response to oxidative stress.-Plant Physiol. 123: 223-233, 2000. Go to original source...

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