Biologia plantarum 50:473-476, 2006 | DOI: 10.1007/s10535-006-0074-5

Micropropagation of Salvia brachyodon through nodal explants

D. Misic1,*, D. Grubisic1, R. Konjevic2
1 Institute for Biological Research "Sinisa Stankovic", Belgrade, Serbia and Montenegro
2 Faculty of Biology, Institute for Botany, Belgrade, Serbia and Montenegro

A protocol for in vitro propagation of Balkan endemic plant Salvia brachyodon Vandas from nodal segments was developed. 6-benzylaminopurine was more effective in axillary buds promotion when compared to thidiazuron. The rooting of regenerated shoots was induced by transferring them to the media supplemented with auxins. All tested auxins (indole-3-acetic acid, indole-3-butyric acid, and α-naphthaleneacetic acid) stimulated the rooting of S. brachyodon shoots. The acclimatization of in vitro rooted shoots was successful.

Keywords: Balkan endemic sage; shoot culture; axillary buds; acclimatization
Subjects: auxins; cytokinins; in vitro culture, micropropagation, propagation; in vitro culture, organogenesis; medicinal plants; nutrient medium, Murashige and Skoog; Salvia brachyodon

Received: January 19, 2005; Accepted: May 10, 2005; Published: September 1, 2006Show citation

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Misic, D., Grubisic, D., & Konjevic, R. (2006). Micropropagation of Salvia brachyodon through nodal explants. Biologia plantarum, 50(3), 473-476. doi: 10.1007/s10535-006-0074-5.
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