Biologia plantarum 50:709-712, 2006 | DOI: 10.1007/s10535-006-0112-3

Interactive effects of UV-B irradiation and triadimefon on nodulation and nitrogen metabolism in Vigna radiata plants

K. Rajendiran1,*, M. P. Ramanujam1
1 Botany Laboratory, Centre for Post Graduate Studies, Pondicherry, India

Supply of aqueous solution of triadimefon (20 mg dm-3) to unstressed green gram plants increased the contents of soluble proteins, amino acids, nitrate and nitrite, and the activity of nitrate reductase in the leaves and nitrate reductase in nodules. The nitrogenase activity in nodules and roots was also increased. Number and fresh mass of nodules and their nitrate and nitrite contents were also higher than those of the controls. In contrast, the UV-B stress (12.2 kJ m-2 d-1) suppressed nodulation and nitrogen metabolism in leaves and roots compared to plants under natural UV-B (10 kJ m-2 d-1). Triadimefon-treated plants did not show such severe inhibitions after exposure to elevated UV-B. Thus triadimefon increased their tolerance to UV-B stress.

Keywords: amelioration of UV-B stress; green gram; nitrate reductase; nitrogenase; triazole compounds
Subjects: amino acids; green gram; nitrate reductase; nitrogenase; nodules, nodulation; proteins; root, rooting; triadimefon; triazole compounds; UV-B radiation, stress

Received: September 21, 2004; Accepted: August 15, 2005; Published: December 1, 2006Show citation

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Rajendiran, K., & Ramanujam, M.P. (2006). Interactive effects of UV-B irradiation and triadimefon on nodulation and nitrogen metabolism in Vigna radiata plants. Biologia plantarum, 50(4), 709-712. doi: 10.1007/s10535-006-0112-3.
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    References

    1. Abbas, S., Zaidi, P.H.: Chemical manipulation through triadimefon against ultraviolet-B induced injury to membrane components of Cicer arietinum L.-J. Physiol. Biochem. 24: 64-66, 1997.
    2. Balakumar, T., Vincent, V.H.B., Paliwal, K.: On the interaction of UV-B radiation (280-315 nm) with water stress in crop plants.-Physiol. Plant. 87: 217-222, 1993. Go to original source...
    3. Bardizick, J.M., Marsh, H.V., Havis, J.R.: Effects of water stress on the activities of three enzymes in maize seedlings.-Plant Physiol. 47: 828-831, 1971. Go to original source...
    4. Caldwell, M.M., Bjorn, L.O., Bornman, J.F., Flint, S.D., Kulandaivelu, G., Teramura, A.H., Tevini, M.: Effects of increased solar ultraviolet radiation on terrestrial ecosystem.-Photochem. Photobiol. 46: 40-52, 1998. Go to original source...
    5. Chimphango, S.B., Musil, C.F., Dakora, F.D.: Response of purely symbiotic and NO3-fed nodulated plants of Lupinus luteus and Vicia atropurpurea to ultraviolet-B radiation.-J. exp. Bot. 54: 1771-1784, 2003. Go to original source...
    6. Fletcher, R.A., Gilley, A., Sankhala, N., Davis, T.D.: Triazoles as plant growth regulators and stress protectants.-In: Janick, J. (ed.): Horticultural Reviews. Vol. 24. Pp. 55-138. John Wiley and Sons, New York 2000. Go to original source...
    7. Fletcher, R.A., Hofstra, G.: Triadimefon-a plant multiprotectant.-Plant Cell Physiol. 26: 775-780, 1985. Go to original source...
    8. Ghisi, R., Trentin, A.R., Masi, A., Ferretti, M.: Carbon and nitrogen metabolism in barley plants exposed to UV-B radiation.-Physiol. Plant. 116: 200-205, 2002. Go to original source...
    9. Gopi, R., Sujatha, B.M., Karikalan, L., Panneerselvam, R.: Triadimefon induced variation in growth and metabolism in the NaCl stressed Vigna unguiculata (L.) seedlings.-Geobios 25: 235-241, 1998.
    10. Guerrero, M.G., Veg, J.M., Losada, M.: The assimilatory nitrate reducing system and its regulation.-Annu. Rev. Plant Physiol. 32: 169-294, 1981. Go to original source...
    11. Jaworski, E.G.: Nitrate reductase in intact plant tissue.-Biochem. biophys. Res. Commun. 43: 1274-1279, 1971. Go to original source...
    12. Kulandaivelu, G., Lingakumar, K.: Molecular targets of UV-B radiation in the photosynthetic membranes.-In: Yunus, M., Pathre, U., Mohanty, P. (ed.): Probing photosynthesis, Mechanisms, Regulation and Adaptation. Pp. 364-378. Taylor and Francis Publications, New York 2000.
    13. Levitt, J.: Salt and ion stress.-In: Levitt, J. (ed.): Responses of Plants to Environmental Stresses. Pp. 489-530. Academic Press, New York 1972.
    14. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent.-J. biol. Chem. 193: 265-275, 1951.
    15. Moore, S., Stein, W.H.: Photometric method for use in the chromatography of amino acids.-J. biol. Chem. 176: 367-388, 1948.
    16. Muthuchelian, K., Nedunchezhian, N., Kulandaivelu, G.: Effect of simulated acid rain on 14CO2 fixation, ribulose-1,5-bisphosphate carboxylase and nitrate and nitrite reductase in Vigna sinensis and Phaseolus mungo.-Photosynthetica 28: 361-367, 1993.
    17. Muthukumarasamy, M., Panneerselvam, R.: Amelioration of NaCl stress by triadimefon in peanut seedlings.-Plant Growth Regul. 22: 157-162, 1997. Go to original source...
    18. Panneerselvam, R., Muthukumarasamy, M., Karikalan, L.: Triadimefon enhances growth and net photosynthetic rate in NaCl stressed plants of Raphanus sativus L.-Photosynthetica 34: 605-609, 1997. Go to original source...
    19. Panneerselvam, R., Muthukumarasamy, M., Rajan, S.N.: Amelioration of NaCl stress by triadimefon in soybean seedlings.-Biol. Plant. 41: 133-137, 1998. Go to original source...
    20. Plaut, Z.: Nitrate reductase activity of wheat seedlings during exposure to and recovery from water stress and salinity.-Physiol. Plant. 30: 212-217, 1974. Go to original source...
    21. Rachel, D., Santhaguru, K.: Impact of UV-B irradiation on growth, nodulation and nitrate assimilation in Vigna mungo L. and Vigna radiata L. Wilczek.-In: Srivastava, G.C., Singh, K., Pal, M. (ed.): Plant Physiology for Sustainable Agriculture. Pp. 294-300. Pointer Publishers, Jaipur 1999.
    22. Rajendiran, K., Ramanujam, M.P.: Efficacy of triadimefon treatment in ameliorating the UV-B stress in green gram.-In: Khan, M. (ed.): National Symposium on Environmental Crisis and Security in the New Millennium. Pp. 41-42. National Environmental Science Academy, New Delhi 2000.
    23. Rajendiran, K., Ramanujam, M.P.: Alleviation of ultraviolet-B radiation-induced growth inhibition of green gram by triadimefon.-Biol. Plant. 46: 621-624, 2003. Go to original source...
    24. Rajendiran, K., Ramanujam, M.P.: Improvement of biomass partitioning, flowering and yield by triadimefon in UV-B stressed Vigna radiata (L.) Wilczek.-Biol. Plant. 48: 145-148, 2004. Go to original source...
    25. Samson, B.M., Chimphango, F.B., Musil, C.F., Dakora, F.D.: Effects of UV-B radiation on plant growth, symbiotic function and concentration of metabolites in three tropical grain legumes.-Functional Plant Biol. 30: 309-318, 2004.
    26. Shriner, D.S., Johnston, J.W.: Effects of simulated acidified rain on nodulation of leguminous plants by Rhizobium spp.-Environ. exp. Bot. 21: 199-209, 1981. Go to original source...
    27. Srivastava, H.S., Fletcher, R.A.: Triadimefon increases nitrate levels and nitrate reductase activity in Canola leaves.-J. exp. Bot. 43: 1267-1271, 1992. Go to original source...
    28. Stewart, W.D.P., Fitzgerald, G.P., Burris, R.H.: In situ studies on nitrogen fixation using the acetylene reduction technique.-Proc. nat. Acad. Sci. USA 58: 2071-2078, 1967. Go to original source...
    29. Tevini, M., Iwanzik, W., Thoma, U.: Some effects of enhanced UV-B radiation on the growth and composition of plants.-Planta 153: 388-394, 1981. Go to original source...
    30. Vu, C.V., Allen, L.H., Garrard, L.A.: Effects of supplementary UV-B radiation on growth and leaf photosynthetic reactions of soybean (Glycine max).-Physiol. Plant. 52: 353-362, 1981. Go to original source...
    31. Woolley, J.T., Hicks, G.P., Hageman, R.H.: Rapid determination of nitrate and nitrite in plant material.-J. agr. Food Chem. 8: 481-482, 1960. Go to original source...
    32. Zar, J.H.: Bio-statistical Analysis.-Prentice-Hall, Englewood Cliffs 1984.

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