Biologia plantarum 51:217-222, 2007 | DOI: 10.1007/s10535-007-0044-6

Effects of benzylaminopurine and irradiance on cytokinin contents, α-tubulin gene expression and cucumber cotyledon expansion

Y. L. Li1,2, Q. H. Ma1,*
1 Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China
2 Graduate School of the Chinese Academy of Sciences, Beijing, China

The fluctuation of endogenous cytokinins was determined in the excised cucumber (Cucumis sativa L.) cotyledons incubated with benzylaminopurine (BA) under irradiance or in darkness. The data indicated that light stimulated the cotyledon expansion compared with dark and BA further enhanced the expansion of cotyledons. However, only BA treatment markedly increased the contents of endogenous cytokinins and induced α-tubulin gene expression. Actinomycin D, a well-known inhibitor of gene transcription, strongly inhibited both light-and BA-induced cotyledon expansions and increase of endogenous cytokinin contents and α-tubulin gene expression. Colchicine, an antimicrotubular reagent, partially inhibited the cotyledon expansion without affecting the cytokinin contents.

Keywords: actinomycin D; colchicine; Cucumis sativa
Subjects: actinomycin D; colchicin; cucumber; Cucumis sativa; cytokinins; in vitro culture, micropropagation, propagation; in vitro culture, regeneration, proliferation, differentiation; polymerase chain reaction (PCR)

Received: September 23, 2005; Accepted: May 17, 2006; Published: June 1, 2007Show citation

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Li, Y.L., & Ma, Q.H. (2007). Effects of benzylaminopurine and irradiance on cytokinin contents, α-tubulin gene expression and cucumber cotyledon expansion. Biologia plantarum, 51(2), 217-222. doi: 10.1007/s10535-007-0044-6.
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