Biologia plantarum 52:49-58, 2008 | DOI: 10.1007/s10535-008-0007-6

The impact of trans-zeatin O-glucosyltransferase gene over-expression in tobacco on pigment content and gas exchange

D. Haisel1, R. Vaňková2, H. Synková1, J. Pospíšilová1,*
1 Institute of Experimental Botany AS CR, Prague 6, Czech Republic
2 Institute of Experimental Botany AS CR, Prague 6, Czech Republic

The responses of tobacco plants over-expressing trans-zeatin O-glucosyltransferase gene under constitutive or senescence-inducible promoter (35S:ZOG1 and SAG12:ZOG1) and of wild type (WT) plants to water stress and subsequent rehydration were compared. In plants sufficiently supplied with water, both transgenics have higher net photosynthetic rate (PN) in upper and middle leaves and higher stomatal conductance (gs) in middle leaves than WT. Water use efficiency (WUE = PN/E) was higher in both transgenics than in WT. During prolonged water stress, both PN and E declined to a similar extent in both transgenics and WT plants. However, 7 d after rehydration PN in SAG:ZOG (upper and middle leaves) and 35S:ZOG (upper leaves) was higher than that in WT plants. Increased content of endogenous CKs in 35S:ZOG plants did not prevent their response to ABA application and the results obtained did not support concept of CK antagonism of ABA-induced stomatal closure. The chlorophyll (Chl) a+b content was mostly higher in both transgenics than in WT. During water stress and subsequent rehydration it remained unchanged in upper leaves, decreased slightly in middle leaves only of WT, while rapidly in lower leaves. Total degradation of Chl, carotenoids and xanthophyll cycle pigments (XCP) was found under severe water stress in lower leaves. Carotenoid and XCP contents in middle and upper leaves mostly increased during development of water stress and decreased after rehydration. While β-carotene content was mostly higher in WT, neoxanthin content was higher in transgenics especially in 35S:ZOG under severe stress and after rehydration. The higher content of XCP and degree of their deepoxidation were usually found in upper and middle leaves than in lower leaves with exception of SAG:ZOG plants during mild water stress.

Keywords: carotenoids; chlorophylls; net photosynthetic rate; Nicotiana tabacum; stomatal conductance; transpiration rate; transgenic plants; xanthophyll cycle pigments
Subjects: abscisic acid (ABA); carotenoids; chlorophyll a,b; cytokinins; gas exchange; Nicotiana tabacum; relative water content (RWC); stomatal conductance; tobacco; transgenic plants; transpiration rate; trans-zeatin; xanthophylls cycle

Received: July 11, 2007; Accepted: September 27, 2007; Published: March 1, 2008Show citation

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Haisel, D., Vaňková, R., Synková, H., & Pospíšilová, J. (2008). The impact of trans-zeatin O-glucosyltransferase gene over-expression in tobacco on pigment content and gas exchange. Biologia plantarum, 52(1), 49-58. doi: 10.1007/s10535-008-0007-6.
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