Biologia plantarum 54:117-120, 2010 | DOI: 10.1007/s10535-010-0017-z

ABA or cadmium induced phytochelatin synthesis in potato tubers

A. Stroiński1,*, T. Chadzinikolau1, K. Giżewska1, M. Zielezińska1
1 Plant Physiology Department, University of Life Sciences, Poznań, Poland

Short-term treatment of potato (Solanum tuberosum L.) tuber discs with CdCl2 solution elevated both the StPCS1 transcript level, phytochelatin synthase (PCS) activity and contents of phytochelatin (PC) and abscisic acid (ABA). Similar effects but less marked were noticed after treatment of tuber tissues with ABA solution. Cd-treatment increased also cysteine (CYS) content but did not change glutathione content. If ABA treatment preceded Cd-treatment, the elevation of CYS and PC contents were limited. The data suggest the participation of ABA in the regulation of PCS.

Keywords: phytochelatins; phytochelatin synthase; Solanum tuberosum
Subjects: abscisic acid (ABA); cadmium; cysteine; glutathione; phytochelatins; phytochelatin synthase; potato; Solanum tuberosum

Received: June 11, 2007; Accepted: September 21, 2008; Published: March 1, 2010Show citation

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Stroiński, A., Chadzinikolau, T., Giżewska, K., & Zielezińska, M. (2010). ABA or cadmium induced phytochelatin synthesis in potato tubers. Biologia plantarum, 54(1), 117-120. doi: 10.1007/s10535-010-0017-z.
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    References

    1. Audran, C., Liotenberg, S., Gonneau, M., North, H., Frey, A., Tap-Waksman, K., Vartanian, N., Marion-Poll, A.: Localisation and expression of zeaxanthin epoxidase mRNA in Arabidopsis in response to drought stress and during seed development. - Aust. J. Plant. Physiol. 28: 1161-1173, 2001. Go to original source...
    2. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. - Anal. Biochem. 72: 248-252, 1976. Go to original source...
    3. Branch, A.D., Benenfeld, B.J., Robertson, H.D.: RNA fingerprinting. - Methods Enzymol. 180: 130-154, 1989. Go to original source...
    4. Chadzinikolau, T., Stroiński, A.: Influence of cadmium-abscisic acid interaction on phytochelatin synthase activity in potato tuber. - Polish J. nat. Sci. 2003 (Suppl. 1): 105, 2003.
    5. Cheng, W.H., Endo, A., Zhou, L., Penney, J., Chen, H.C., Arroyo, A., Leon, P., Nambara, E., Asami T., Seo, M., Koshiba, T., Shenn, J.: A unique short-chain dehydrogenase/reductase in Arabidopsis glucose signaling and abscisic acid biosynthesis and functions. - Plant Cell 14: 2723-2743, 2002. Go to original source...
    6. Clemens, C.: Evolution and function of phytochelatin synthases. - J. Plant Physiol. 163: 319-332, 2006. Go to original source...
    7. Clemens S., Kim E.J., Neumann, D., Schroeder, J.I.: Tolerance to toxic metals by a gene family of phytochelatin synthases from plants and yeast. - EMBO J. 18: 3325-3333, 1999. Go to original source...
    8. Cobbett, C., Goldsbrough, P.: Phytochelatins and metallothioneins: roles in heavy metal detoxification and homeostasis. - Annu. Rev. Plant Biol. 53: 159-182, 2002. Go to original source...
    9. Eckardt, N.A.: Abscisic acid biosynthesis gene underscores the complexity of sugar, stress, and hormone interactions. - Plant Cell 14: 2645-2649, 2002. Go to original source...
    10. El-Shintinawy, F., El-Ansary, A.: Differential effect of Cd2+ and Ni2+ on amino acid metabolism in soybean seedlings. - Biol. Plant. 43: 79-84, 2000. Go to original source...
    11. Fediuc, E., Lips, H., Erdei, L.: O-acetylserine (thiol) lyase activity in Pragmites and Typha plants under cadmium and NaCl stress conditions and the involvement of ABA in the stress response. - J Plant Physiol. 162: 865-872, 2005. Go to original source...
    12. Giraudat, J., Parcy, F., Bertauche, N., Gosti, F., Leung, J., Morris, P.C., Bouvier-Durand, M., Vartanian, N.: Current advances in abscisic acid action and signaling. - Plant mol. Biol. 26: 1557-1577, 1994. Go to original source...
    13. Grill, E., Löffler, S., Winnacker, E.-L., Zenk, M.H.: Phytochelatins, the heavy-metal binding peptides of plants, are synthesized from glutathione by a specific glutamylcysteine dipeptydyl transpeptidase (phytochelatin synthase). - Proc. nat. Acad. Sci. USA 86: 6838-6842, 1989. Go to original source...
    14. Ha, S.B., Smith, A.P., Howden, R., Dietrich, W.M., Bugg, S., O'Connell, M.J., Goldsbrough, P.B., Cobbett, C.S.: Phytochelatin synthase genes from Arabidopsis and the yeast, Schizosaccharomyces pombe. - Plant Cell 11: 1153-1164, 1999. Go to original source...
    15. Hendry, G.A.F., Baker, A.J.M., Ewart, C.F., Cadmium tolerance and toxicity, oxygen radical processes and molecular damage in cadmium-tolerant and cadmium-sensitive clones of Holcus lanatus. - Acta bot. neerl. 41: 271-281, 1992. Go to original source...
    16. Hsu, Y.T., Kao, C.H.: Role of abscisic acid in cadmium tolerance in rice (Oryza sativa L.) seedlings. - Plant Cell Environ. 26: 867-874, 2003. Go to original source...
    17. Hsu, Y.T., Kao, C.H.: Abscisic acid accumulation and cadmium tolerance in rice seedlings. - Physiol. Plant. 124: 71-80, 2005. Go to original source...
    18. Hsu, Y.T., Kuo, M.C., Kao, C.H.: Cadmium-induced ammonium ion accumulation of rice seedlings at high temperature is mediated through abscisic acid. - Plant Soil 287: 267-277, 2006. Go to original source...
    19. Jiang, W.S., Liu, D.H., Xu, P.: Cd-induced system of defence in the garlic root meristematic cells. - Biol. Plant. 53: 369-372, 2009. Go to original source...
    20. Karcz, W., Kurtyka R. Effect of cadmium on growth, proton extrusion and membrane potential in maize coleoptile segments. - Biol. Plant. 51: 713-719, 2007. Go to original source...
    21. Knight H., Knight M.R.: Abiotic stress signalling pathways: specificity and cross-talk. - Trends Plant Sci. 6: 262-267, 2001. Go to original source...
    22. Maksimović, I., Kastori, R., Krstić, L., Luković, J.: Steady presence of cadmium and nickel affects root anatomy, accumulation and distribution of essential ions in maize seedlings. - Biol. Plant. 51: 589-592, 2007. Go to original source...
    23. Maitani, T., Kubota, H., Sato, K., Yamada, T.: The composition of metals bound to class III metallothionein (phytochelatin and its desglycyl peptide) induced by various metals in root cultures of Rubia tinctorum. - Plant Physiol. 110: 1145-1150, 1996. Go to original source...
    24. Moore, R. Abscisic acid is not necessary for gravitropism in primary roots of Zea mays. - Ann. Bot. 66: 281-283, 1990. Go to original source...
    25. Nocito, F.F., Pirovano, L., Cocucci, M., Sacchi, G.A.: Cadmium-induced sulfate uptake in maize roots. - Plant Physiol. 129: 1872-1879, 2002.
    26. Nuc P.W., Chadzinikoalu, T., Nuc, K., Stroiński, A.: Solanum tuberosum mRNA for phytochelatin synthase (pcs1 gene). Accession: AJ548472. - EMBL/GenBank/DDBJ databases, 2003.
    27. Sanita di Toppi, L., Gabrielli, R.: Response to cadmium in higher plants. - Environ. exp. Bot. 41: 105-130, 1999. Go to original source...
    28. Shinozaki, K., Yamagucchi, K., Seki, M.: Regulatory network of gene expression in drought and cold stress responses. Curr. Opin. Plant Biol. 6: 410-417, 2003. Go to original source...
    29. Skórzyńska-Polit, E., Drążkiewicz, M., Krupa, Z.: The activity of the antioxidative system in cadmium-treated Arabidopsis thaliana. - Biol. Plant. 47: 71-78, 2003. Go to original source...
    30. Stiborová, M., Ditrichová, M., Březinová, A.: Effects of heavy metal ions on growth and biochemical characteristic of photosynthesis of barley and maize seedling. - Biol. Plant. 29: 453-467, 1987. Go to original source...
    31. Stroiński, A., Floryszak-Wieczorek, J, Woźny, A.: Effects of cadmium on the host-pathogen system. I. Alteration of potato leaves and Phytophthora infestans relations. - Biochem. Physiol. Pflanz. 186: 43-54, 1990. Go to original source...
    32. Stroiński, A., Kozłowska, M. Cadmium-induced oxidative stress in potato tuber. - Acta Soc. Bot. Polon. 66: 189-195, 1997.
    33. Stroiński, A., Zielezińska, M.: Cadmium effect on hydrogen peroxide, glutathione and phytochelatins levels in potato tuber. - Acta Physiol. Plant. 19: 127-136, 1997. Go to original source...
    34. Stroiński, A., Zielezińska, M.: Cadmium and oxidative stress influence on phytochelatin synthase activity in potato tuber. - Acta Physiol. Plant. 23: 157-160, 2001. Go to original source...
    35. Tukendorf, A.: Changes in glutathione in detoxification of cadmium and excess of copper in spinach plants. - Acta Physiol. Plant. 15: 175-183, 1993.
    36. Tukendorf, A., Rauser, W.E.: Changes in glutathione and phytochelatins in roots of maize seedlings exposed to cadmium. - Plant Sci. 70: 155-166, 1990. Go to original source...
    37. Vatamaniuk, O.K., Mar, S., Lu, Y.P., Rea, P.A.: Mechanism of heavy metal ion activation of phytochelatin (PC) synthase: blocked thiols are sufficient for PC synthase-catalyzed transpeptidation of glutathione and related thiol peptides. - J. biol. Chem. 275: 31451-31459, 2000. Go to original source...
    38. Xiong, L., Schumaker, K.S., Zhu, J.-K.: Cell signaling during cold, drought and salt stress. - Plant Cell Suppl.: S165-S183, 2002. Go to original source...
    39. Xiong, L., Zhu, J.-K.: Regulation of abscisic acid biosynthesis. - Plant Physiol. 133: 29-36, 2003. Go to original source...

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