Biologia plantarum 54:198-200, 2010 | DOI: 10.1007/s10535-010-0035-x

Genetic diversity of durum wheat as determined by AFLP in fluorescence

F. Sciacca1,*, C. Fichera1, S. Di Silvestro2, E. Conte2, M. Palumbo1
1 Agricultural Research Council, Mediterranean Crops Research Center, Acireale, Italy
2 Science and Technology Park of Sicily, Zona Industriale Blocco Palma I, Catania, Italy

The DNA of fifteen Italian cultivars of durum wheat (Triticum turgidum L. ssp. durum) were analyzed by in fluorescence amplified fragment length polymorphism (fAFLP) in order to obtain the characteristic fingerprintings of genotypes and assess their genetic relatedness. Among 64 combinations of fluorescence labelled primers, three different combinations were chosen as producing a total of 6630 AFLP fragments, 2277 (34.3 %) of them being polymorphic. By using this fAFLP methodology a DNA fingerprinting of each durum wheat cultivar was generated for genotype identification. Analysis of the genetic relationships show the low variability among durum wheat cultivars.

Keywords: genetic relationship; genotype identification; pattern analysis; Triticum turgidum L. ssp. durum
Subjects: amplified fragment length polymorphism (AFLP); fluorescence AFLP; genetic diversity; Triticum turgidum; UPGMA dengrogram; wheat

Received: February 11, 2008; Accepted: June 21, 2008; Published: March 1, 2010Show citation

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Sciacca, F., Fichera, C., Silvestro, S., Conte, E., & Palumbo, M. (2010). Genetic diversity of durum wheat as determined by AFLP in fluorescence. Biologia plantarum, 54(1), 198-200. doi: 10.1007/s10535-010-0035-x.
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