Biologia plantarum 54:391-394, 2010 | DOI: 10.1007/s10535-010-0071-6

In vitro propagation of Drosera intermedia in a single step

T. Grevenstuk1, N. Coelho1, S. Gonçalves1, A. Romano1,*
1 Faculty of Sciences and Technology, University of Algarve and Institute for Biotechnology and Bioengineering (IBB/CGB - UTAD), Faro, Portugal

A simple and efficient protocol for the micropropagation of Drosera intermedia, using cultures initiated from in vitro produced seedlings, is described. Shoot proliferation was significantly influenced by Murashige and Skoog (MS) macronutrient concentration, showing higher multiplication rates for 1/4 MS (the lowest concentration), but was not affected by the addition of 0.1 mg dm-3 kinetin. In all cases a multiplication percentage above 90 % was recorded. High rooting percentages (up to 100 %) were obtained in multiplication phase on 1/4 MS medium without growth regulators. In average 15.8 plantlets per initial shoot was produced after 8 weeks of culture. All plantlets were successfully acclimatized to ex vitro conditions, exhibiting normal development.

Keywords: carnivorous plant; Droseraceae; kinetin
Subjects: auxins; cytokinins; Drosera intermedia; ex vitro transfer; in vitro culture, direct organogenesis; in vitro culture, rooting; in vitro culture, seedlings

Received: October 1, 2008; Accepted: March 11, 2009; Published: June 1, 2010Show citation

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Grevenstuk, T., Coelho, N., Gonçalves, S., & Romano, A. (2010). In vitro propagation of Drosera intermedia in a single step. Biologia plantarum, 54(2), 391-394. doi: 10.1007/s10535-010-0071-6.
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