Biologia plantarum 2010, 54:547-550 | DOI: 10.1007/s10535-010-0097-9

Storage and conversion of Eclipta alba synseeds and RAPD analysis of the converted plantlets

A. Ray1,2,*, S. Bhattacharya1
1 Department of Botany, Bose Institute, Kolkata, India
2 NCBS-TIFR, GKVK Campus, Bangalore, India

The encapsulated shoot tips and nodal segments of Eclipta alba were stored at 4, 12 and 20 °C under irradiance of 1.5 gmmol m-2 s-1 and high conversion was observed in synseeds stored at 4 °C for 8 weeks. Duration of storage was extended up to 12 weeks by decreasing sucrose concentration in the alginate matrix from 3 to 1 or 2 % and conversion frequency was 71.2-76.1 %. Synseed-derived plantlets survived by 100 % in ex vitro conditions. RAPD analysis revealed uniform amplification profile in donor and synseed derived plantlets.

Keywords: alginate matrix; encapsulated explants; ex vitro transfer; genetic uniformity; sucrose
Subjects: alginate matrix; Eclipta alba; encapsulation; ex vitro transfer; random amplified polymorphic DNA (RAPD); synthetic seeds

Received: November 7, 2008; Accepted: June 2, 2009; Published: September 1, 2010Show citation

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Ray, A., & Bhattacharya, S. (2010). Storage and conversion of Eclipta alba synseeds and RAPD analysis of the converted plantlets. Biologia plantarum, 54(3), 547-550. doi: 10.1007/s10535-010-0097-9.
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