Biologia plantarum 2012, 56:377-382 | DOI: 10.1007/s10535-012-0103-5

Na+/H+ antiport activity in plasma membrane and tonoplast vesicles isolated from NaCl-treated cucumber roots

K. Kabała1, M. Janicka-Russak1,*
1 Department of Plant Physiology, Institute of Plant Biology, University of Wrocław, Wrocław, Poland

Sodium/proton antiporter activity in the plasma membrane and tonoplast of cucumber seedling roots treated with 200 mM NaCl for 24 h was determined. It was observed that plasma membrane and tonoplast antiporter activity was only present in membranes from salt-treated plants. In addition, the plasma membrane antiporter protein was present in membranes after induction with NaCl, whereas tonoplast antiporter protein was observed in control and at elevated level in NaCl-treated plants. Moreover, based on the affinity of studied antiporter proteins to sodium ions, it could be assumed that excess sodium ions are firstly translocated from the cytosol to the vacuole and then excluded to the apoplast through the plasma membrane.

Keywords: antiporter proteins; salt stress; sodium compartmentalization
Subjects: antiporter proteins; salt stress; sodium compartmentalization; plasma membrane; tonoplast; ATP hydrolysis; proton transport
Species: Cucumis sativus

Received: November 23, 2010; Accepted: June 22, 2011; Published: June 1, 2012Show citation

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Kabała, K., & Janicka-Russak, M. (2012). Na+/H+ antiport activity in plasma membrane and tonoplast vesicles isolated from NaCl-treated cucumber roots. Biologia plantarum, 56(2), 377-382. doi: 10.1007/s10535-012-0103-5.
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