Biologia plantarum 2013, 57:559-562 | DOI: 10.1007/s10535-012-0301-1

Extent of cross-fertilization in Orobanche cumana Wallr.

M. I. Rodríguez-Ojeda1, J. M. Fernández-Martínez2, L. Velasco2, B. Pérez-Vich2,*
1 Koipesol Semillas S.A. Avda. San Francisco Javier 24-7, Sevilla, Spain
2 Instituto de Agricultura Sostenible (IAS-CSIC), Córdoba, Spain

Sunflower broomrape (Orobanche cumana Wallr.) is considered a self-fertilizing species, but there is no indication as to whether it is strictly self-fertilized or that it presents some extent of cross-fertilization. The objective of this research was to measure the rate of cross-fertilization in O. cumana using an unpigmented recessive mutant as a visual marker. A pot and a field experiment in which single unpigmented plants were surrounded by a large number of pigmented plants were conducted. Occurrence of F1 hybrids, readily distinguishable from unpigmented plants in the progenies of unpigmented plants provided a direct measurement of the cross-fertilization rate. Progenies of unpigmented plants contained 21.5 % of F1 hybrids in the pot experiment and 28.8 % in the field experiment. The results revealed that O. cumana is a partially allogamous species, which has great relevance for understanding the genetic structure and dynamics of populations and, ultimately, race evolution in this parasitic plant.

Keywords: allogamy; anthocyaninless; autogamy; sunflower broomrape; unpigmented plants.
Subjects: fertilization; allogamy; anthocyanins; autogamy; unpigmented plants; hybrids; sunflower broomrape
Species: Orobanche cumana

Received: June 5, 2012; Accepted: October 15, 2012; Published: September 1, 2013Show citation

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Rodríguez-Ojeda, M.I., Fernández-Martínez, J.M., Velasco, L., & Pérez-Vich, B. (2013). Extent of cross-fertilization in Orobanche cumana Wallr. Biologia plantarum, 57(3), 559-562. doi: 10.1007/s10535-012-0301-1.
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