Biologia plantarum 2013, 57:793-796 | DOI: 10.1007/s10535-013-0357-6

Chloroplast ultrastructure of Hypericum perforatum plants regenerated in vitro after cryopreservation

D. Stoyanova-Koleva1,*, M. Stefanova1, E. Čellárová3, V. Kapchina-Toteva2
1 Department of Botany, Faculty of Biology, Sofia University "St. Kliment Ohridski", Sofia, Bulgaria
2 Department of Plant Physiology, Faculty of Biology, Sofia University "St. Kliment Ohridski", Sofia, Bulgaria
3 Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafárik University in Košice, Košice, Slovakia

The ultrastructure of leaf mesophyll cells of in vitro cultured Hypericum perforatum L. plants regenerated after cryopreservation was studied. Electron microscopy analysis revealed that the chloroplasts in plants pretreated with abscisic acid and regenerated after cryopreservation were round, with increased amount of starch, rather small volume of the thylakoid system, and destroyed envelope. Plants pretreated with 0.3 M mannitol and cooled at rates of 0.1 or 0.3 °C min-1 possessed chloroplasts with high starch content that resulted in a reduction of a membrane system. However, the pretreatment with 0.3 M mannitol and cooling at a rate of 0.2 °C min-1 was the best as chloroplast ultrastructure resembled the controls regenerated without cryopreservation.

Keywords: ABA; mannitol; medicinal plant; micropropagation; St. John's wort
Subjects: chloroplast; ultrastructure; in vitro culture; cryopreservation; abscisic acid; mannitol
Species: Hypericum perforatum

Received: January 21, 2013; Accepted: April 29, 2013; Published: December 1, 2013Show citation

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Stoyanova-Koleva, D., Stefanova, M., Čellárová, E., & Kapchina-Toteva, V. (2013). Chloroplast ultrastructure of Hypericum perforatum plants regenerated in vitro after cryopreservation. Biologia plantarum, 57(4), 793-796. doi: 10.1007/s10535-013-0357-6.
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