biologia plantarum

Biologia plantarum 2015, 59:154-162 | DOI: 10.1007/s10535-014-0480-z

Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress

H. F. Causin^1,*, C. F. Marchetti¹, L. B. Pena², S. M. Gallego², A. J. Barneix³

¹ Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina

² Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina

³ Instituto de Suelos, CIRN-INTA, N. Repetto y de los Reseros, Castelar, Buenos Aires, Argentina

In shaded wheat (Triticum aestivum L.) leaves, the suppression of blue radiation (BR) triggers senescence. This phenomenon is correlated to an increase in oxidative stress symptoms and a decrease of catalase (CAT) activity, among other traits. Previous data suggest that the radiation signal transduction pathway may involve changes in Ca²⁺ and H₂O₂ homeostasis. For better a understanding of the interaction among the spectral composition of radiation, Ca²⁺ availability, and the antioxidant metabolism in the regulation of shade-induced senescence, detached wheat leaves were placed in a growth chamber and exposed to either blue (B, high BR transmittance) and/or green (G, very low BR transmittance) Lee® filters in the absence or presence of 0.8 mM verapamil (a Ca²⁺ channels blocker), 4.0 mM EGTA (a Ca²⁺chelator), or 8.0 mM 3-amino-1,2,4-triazole (a CAT inhibitor). At defined time points, the leaf samples were analyzed for changes in chlorophyll content, specific activities of CAT, ascorbate peroxidase (APX), and guaiacol peroxidase (POX), CAT isozymes, and gene expression of CAT1, CAT2, and two senescence markers (TaSAG1 and TaSAG3). BR transmittance decreased the chlorophyll degradation rate and SAG genes expression either in leaves continuously exposed under the B filter, as well as in leaves previously exposed under the G filter. The effect of BR was associated with the maintenance of a high CAT (but not APX and POX) activity, and it was suppressed either in the presence of 3-AT or when Ca²⁺ availability was decreased. BR altered the CAT activity both at the transcriptional and at the posttranscriptional level. Nevertheless, different responses of CAT isozymes and CAT genes expression profiles to specific treatment combinations indicate that they differed in their regulatory pathways.

Keywords: antioxidatnts; blue radiation; calcium; gene expression; Triticum aestivum

Subjects: catalase; senescence; gene expression; chlorophyll; ascorbate peroxidase; peroxidase; blue radiation; shading; wheat

Species: Triticum aestivum

Received: May 20, 2014; Revised: August 7, 2014; Accepted: August 12, 2014; Published: January 1, 2015Show citation