Biologia plantarum 60:655-666, 2016 | DOI: 10.1007/s10535-016-0631-5

Cloning PIP genes in drought-tolerant vetiver grass and responses of transgenic VzPIP2;1 soybean plants to water stress

S. B. Hu1, Q. Zhou1,2, J. An1, B. J. Yu1,*
1 College of Life Sciences, Nanjing Agricultural University, Nanjing, P.R. China
2 Key Laboratory of Plant Resource Conservation and Utilization, Jishou University, Jishou, P.R. China

Vetiver grass [Vetiveria zizanioides (L.) Nash] displays comprehensive abiotic stress tolerance closely related to fine maintenance of plant water relation mediated by plasma membrane intrinsic proteins (PIPs). Two open reading frame sequences of PIPs (867 and 873 bp) were cloned from vetiver grass and named as VzPIP1;1 and VzPIP2;1, respectively. Expression of green fluorescent protein revealed only subcellular localization of VzPIP2;1 in the plasma membrane. Agrobacterium tumefaciens mediated transgenic (VzPIP2;1) soybean plants had a higher water content in above-ground parts under sufficient water supply through enhancing transpiration as compared to the non-transgenic plants but displayed a more severe drought injury because of a lower photosynthesis and a higher transpiration rate. However, A. rhizogenes mediated transgenic soybean plants kept a higher water content in above-ground parts by improving root water transport and kept a more effective photosynthesis under normal and drought conditions.

Keywords: Agrobacterium; Glycine max; root hydraulic conductivity; transpiration; Vetiveria zizanioides
Subjects: aquaporins; plasma membrane intrinsic protein; hydraulic conductance; transpiration rate; stomatal conductance; net photosynthetic rate; chlorophyll a fluorescence; electrolyte leakage; transgenic plans; wild soybean; abscisic acid response element
Species: Allium cepa; Glycine max; Vetiveria zizanoides; Agrobacterium

Received: May 13, 2015; Revised: January 16, 2016; Accepted: January 18, 2016; Published: December 1, 2016Show citation

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Hu, S.B., Zhou, Q., An, J., & Yu, B.J. (2016). Cloning PIP genes in drought-tolerant vetiver grass and responses of transgenic VzPIP2;1 soybean plants to water stress. Biologia plantarum, 60(4), 655-666. doi: 10.1007/s10535-016-0631-5.
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