biologia plantarum

Biologia plantarum 61:611-621, 2017 | DOI: 10.1007/s10535-017-0723-x

Two novel WRKY genes from Juglans regia, JrWRKY6 and JrWRKY53, are involved in abscisic acid-dependent stress responses

G. Y. Yang^1,3, W. H. Zhang², Y. D. Sun¹, T. T. Zhang¹, D. Hu¹, M. Z. Zhai^1,*

¹ Laboratory of Walnut Research Center, College of Forestry, Northwest A & F University, Yangling, Shaanxi, P.R. China

² Agronomy College, Heilongjiang Bayi Agricultural University, Daqing, P.R. China

³ Hunan Research Center of Engineering Technology for Utilization of Environmental and Resources Plant, Changsha, Hunan, P.R. China

Genes encoding plant WRKY transcription factors are important for stress response. In the current study, two WRKY transcription factor genes (JrWRKY6 and JrWRKY53) were identified from walnut (Juglans regia L.), and their function and involvement in stress responses were characterized. Under NaCl stress, JrWRKY6 and JrWRKY53 were upregulated in a short time (within 6 h of seedling exposure to salt) except in roots, in which the highest induction occurred at 24 and 48 h of salt exposure. The gene expression patterns under polyethylene glycol stress were similar to those under NaCl stress. Under heat stress, both genes were induced in all tissues, except for JrWRKY6 in leaf tissue of seedlings treated for 24 and 48 h. Both genes were also induced in all plants exposed to cold stress, except for JrWRKY6 in root tissue of seedlings exposed for 6 h and JrWRKY53 in root tissue exposed for 48 h. JrWRKY6 and JrWRKY53 also showed varied responses to abscisic acid (ABA), with the maximum expression being for JrWRKY6 in the roots of plants treated for 1 h, and JrWRKY53 in the leaves of plants treated for 3 h. Furthermore, under NaCl, sorbitol, heat, cold, and ABA treatments, yeast cells transformed with JrWRKY6 and JrWRKY53 showed an improved growth activity and density relative to the empty-vector-containing control yeast. Moreover, JrWRKY6 or JrWRKY53 could bind to the W-box motif. These results suggest that JrWRKY6 and JrWRKY53 can response positively to abiotic stressors and improve the plant tolerance to salinity, osmotic stress, and abnormal temperatures in a mechanism that likely involves the ABA signalling pathway and W-box binding activity.

Keywords: cold; gene expression; heat; NaCl; polyethylene glycol; walnut

Subjects: WRKY genes; abscisic acid; cold stress; heat stress; salinity; polyethylene glycol; phylogenetic tree; sorbitol; walnut

Species: Juglans regia

Received: April 11, 2016; Revised: November 22, 2016; Accepted: December 9, 2016; Published: December 1, 2017Show citation