Biologia plantarum 54:121-125, 2010 | DOI: 10.1007/s10535-010-0018-y

Direct somatic embryogenesis and plant regeneration from immature explants of chickpea

S. Kiran Ghanti1,3,*, K. G. Sujata1, M. Srinath Rao1, P. B. Kavi Kishor2
1 Department of Botany, Gulbarga University, Gulbarga, India
2 Department of Genetics, Osmania University, Hyderabad, India
3 Department of Crop physiology, University of Agricultural Science, GKVK, Karnataka, Bangalore, India

A protocol for plant regeneration via somatic embryogenesis was developed in two chickpea (Cicer arietinum L.) cultivars ICCV-10 and Annigeri. Somatic embryos were induced from immature cotyledons on Murashige and Skoog's (MS) medium supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), α-naphthaleneacetic acid (NAA) and picloram alone or in combination with 0.5 - 2.0 mg dm-3 N6-benzylaminopurine (BA) or kinetin (KIN). NAA was better for somatic embryo induction compared to other auxins. The well formed, cotyledonary shaped embryos germinated into plantlets with 36.6 % frequency on MS medium supplemented with 2.0 mg dm-3 BA + 0.5 mg dm-3 abscisic acid (ABA). The frequency of embryogenesis and plantlet regeneration was higher in cv. ICCV-10 as compared to cv. Annigeri. Regenerated plants were transferred to soil (40 % survival) and grown to maturity. Histological studies of explants at various developmental stages of somatic embryogenesis reveled that somatic embryos developed directly from the cotyledon cells and they were single cell origin.

Keywords: abscisic acid; auxins; cytokinins; germination; histology; mature somatic embryos
Subjects: abscisic acid (ABA); auxins; chickpea; Cicer arietinum; cytokinins; in vitro culture, somatic embryogenesis

Received: December 7, 2007; Accepted: August 5, 2008; Published: March 1, 2010Show citation

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Ghanti, S., Sujata, K.G., Rao, M., & Kishor, P.B. (2010). Direct somatic embryogenesis and plant regeneration from immature explants of chickpea. Biologia plantarum, 54(1), 121-125. doi: 10.1007/s10535-010-0018-y.
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