Biologia plantarum 54:159-163, 2010 | DOI: 10.1007/s10535-010-0026-y

Antioxidative enzymatic protection in leaves of two contrasting cowpea cultivars under salinity

J. M. Maia1, C. E. Costa de Macedo2, E. L. Voigt1, J. B. S. Freitas1, J. A. G. Silveira1,*
1 Departamento de Bioquímica e Biologia Molecular, Instituto Nacional de Ciência e Tecnologia em Salinidade, Universidade Federal do Ceará, CP 6033, Fortaleza, Ceará, Brazil
2 Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil

The aim of this work was to investigate the role of the antioxidant enzymes in salt tolerance comparing the salt-sensitive (Pérola) and a salt-tolerant (Pitiúba) cultivar of cowpea [Vigna unguiculata (L.) Walp.]. Salt stress (100 mM NaCl for 8 d) reduced the leaf growth rate more in the sensitive cultivar. The salt-induced decrease in the relative water content, Na+ accumulation and increase in leaf electrolyte leakage was similar in both cultivars. Salt stress induced a higher increase in the activities of superoxide dismutase (SOD), ascorbate peroxidase (APX) and phenol peroxidase (POX) in the tolerant cultivar than in sensitive one.

Keywords: ascorbate peroxidase; oxidative damage; peroxidase; salt stress; salt tolerance; superoxide dismutase; Vigna unguiculata
Subjects: ascorbate peroxidase; catalase; cowpea; electrolyte leakage; leaf growth; peroxidase; relative water content (RWC); salinity; sodium; superoxide dismutase (SOD); thiobarbituric acid reactive substances; Vigna unguiculata

Received: June 23, 2008; Accepted: December 22, 2008; Published: March 1, 2010Show citation

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Maia, J.M., de Macedo, C.E.C., Voigt, E.L., Freitas, J.B.S., & Silveira, J.A.G. (2010). Antioxidative enzymatic protection in leaves of two contrasting cowpea cultivars under salinity. Biologia plantarum, 54(1), 159-163. doi: 10.1007/s10535-010-0026-y.
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    References

    1. Alscher, R.G., Erturk, N., Heath, L.S.: Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. - J. exp. Bot. 53: 1331-1341, 2002. Go to original source...
    2. Apel, K., Hirt, H.: Reactive oxygen species: metabolism, oxidative stress, and signal transduction. - Annu. Rev. Plant Biol. 55: 373-399, 2004. Go to original source...
    3. Asada, K.: Ascorbate peroxidase - a hydrogen peroxidescavenging enzyme in plants. - Physiol. Plant. 85: 235-241, 1992. Go to original source...
    4. Ashraf, M., Ali, Q.: Relative membrane permeability and activities of some antioxidant enzymes as the key determinants of salt tolerance in canola (Brassica napus L.). - Environ. exp. Bot. 63: 266-273, 2008. Go to original source...
    5. Attia, H., Arnaud, N., Karray, N., Lachaal, M.: Long-term effects of mild salt stress on growth, ion accumulation and superoxide dismutase expression of Arabidopsis rosette leaves. - Physiol. Plant. 132: 293-305, 2008. Go to original source...
    6. Azevedo-Neto, A.D.D., Prisco, J.T., Eneas, J., De Abreu, C.E.B., Gomes, E.: Effect of salt stress on antioxidative enzymes and lipid peroxidation in leaves and roots of salttolerant and salt-sensitive maize genotypes. - Environ. exp. Bot. 56: 87-94, 2006. Go to original source...
    7. Azevedo-Neto, A.D.D., Prisco, J.T., Eneas, J., Medeiros, J.V.R., Gomes, E.: Hydrogen peroxide pre-treatment induces salt stress acclimation in maize plants. - J. Plant Physiol. 162: 1114-1122, 2005. Go to original source...
    8. Bacon, M.A., Thompson, D.S., Davies, W.J.: Can cell wall peroxidase activity explain the leaf growth response of Lolium temulentum L. during drought? - J. exp. Bot. 48: 2075-2085, 1997. Go to original source...
    9. Baťková, P., Pospíšilová, J., Synková, H.: Production of reactive oxygen species and development of antioxidative systems during in vitro growth and ex vitro transfer. - Biol. Plant. 52: 413-422, 2008.
    10. Blum, A., Ebercon, A.: Cell-membrane stability as a measure of drought and heat tolerance in wheat. - Crop Sci. 21: 43-47, 1981. Go to original source...
    11. Bogdanović, J., Radotić, K., Mitrović, A. Changes in activities of antioxidant enzymes during Chenopodium murale seed germination. - Biol. Plant. 52: 396-400, 2008. Go to original source...
    12. Cairo, P.A.R.: Relações Hídricas de Plantas. [Hydric Relations of Plants]. - UESB, Vitória da Conquista 1995. [In Port.]
    13. Cavalcanti, F.R., Lima, J.P.M.S., Ferreira-Silva, S.L., Viegas, R.A., Silveira, J.A.G.: Roots and leaves display contrasting oxidative response during salt stress and recovery in cowpea. - J. Plant Physiol. 164: 591-600, 2007. Go to original source...
    14. Cavalcanti, F.R., Oliveira, J.T.A., Martins-Miranda, A.S., Viegas, R.A., Silveira, J.A.G.: Superoxide dismutase, catalase and peroxidase activities do not confer protection against oxidative damage in salt-stressed cowpea leaves. - New Phytol. 163: 563-571, 2004. Go to original source...
    15. Dionisio-Sese, M.L., Tobita, S.: Antioxidant responses of rice seedlings to salinity stress. - Plant Sci. 135: 1-9, 1998. Go to original source...
    16. Edreva, A.: Generation and scavenging of reactive oxygen species in chloroplasts: a submolecular approach. - Agr. Ecosyst. Environ. 106: 119-133, 2005. Go to original source...
    17. Foyer, C.H., Noctor, G.: Oxygen processing in photosynthesis: regulation and signalling. - New Phytol. 146: 359-388, 2000. Go to original source...
    18. Foyer, C.H., Noctor, G.: Redox sensing and signalling associated with reactive oxygen in chloroplasts, peroxisomes and mitochondria. - Physiol. Plant. 119: 355-364, 2003. Go to original source...
    19. Freitas, J.B.S.: Respostas fisiológicas ao estresse salino de duas cultivares de feijão-caupi. [Physiologial responses of two contrasting cultivars of cowpea under salt stress]. - UFC, Fortaleza - Ceará 2006. [In Port.]
    20. Gossett, D.R., Millhollon, E.P., Lucas, M.C.: Antioxidant response to NaCl stress in salt-tolerant and salt-sensitive cultivars of cotton. - Crop Sci. 34: 706-714, 1994. Go to original source...
    21. Havir, E.A., McHale, N.A.: Biochemical and developmental characterization of multiple forms of catalase in tobaccoleaves. - Plant Physiol. 84: 450-455, 1987. Go to original source...
    22. Heath, R.L., Packer, L.: Photoperoxidation in isolated chloroplasts 2. Role of electron transfer. - Arch. Biochem. Biophys. 125: 169-198, 1968. Go to original source...
    23. Hernández, J.A., Ferrer, M.A., Jimenez, A., Barcelo, A.R., Sevilla, F.: Antioxidant systems and O2--./H2O2 production in the apoplast of pea leaves. Its relation with salt-induced necrotic lesions in minor veins. - Plant Physiol. 127: 817-831, 2001. Go to original source...
    24. Imlay, J.A.: Pathways of oxidative damage. - Annu. Rev. Microbiol. 57: 395-418, 2003. Go to original source...
    25. Koshiba, T.: Cytosolic ascorbate peroxidase in seedlings and leaves of maize (Zea mays). - Plant Cell Physiol. 34: 713-721, 1993. Go to original source...
    26. Lin, C.C., Kao, C.H.: Osmotic stress-induced changes in cell wall peroxidase activity and hydrogen peroxide level in roots of rice seedlings. - Plant Growth Regul. 37: 177-183, 2002. Go to original source...
    27. Mandhania, S., Madan, S., Sawhney, V.: Antioxidant defense mechanism under salt stress in wheat seedlings. - Biol. Plant. 50: 227-231, 2006. Go to original source...
    28. Meloni, D.A., Oliva, M.A., Martinez, C.A., Cambraia, J.: Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. - Environ. exp. Bot. 49: 69-76, 2003. Go to original source...
    29. Möller, I.M., Jensen, P.E., Hansson, A.: Oxidative modifications to cellular components in plants. - Annu. Rev. Plant Biol. 58: 459-481, 2007. Go to original source...
    30. Passardi, F., Penel, C., Dunand, C.: Performing the paradoxical: how plant peroxidases modify the cell wall. - Trend. Plant Sci. 9: 534-540, 2004. Go to original source...
    31. Procházková, D., Wilhelmová, N.: Leaf senescence and activities of the antioxidant enzymes. - Biol. Plant. 51: 401-406, 2007. Go to original source...
    32. Radic, S., Radic-Stojkovic, M., Pevalek-Kozlina, B.: Influence of NaCl and mannitol on peroxidase activity and lipid peroxidation in Centaurea ragusina L. roots and shoots. - J. Plant Physiol. 163: 1284-1292, 2006. Go to original source...
    33. Sairam, R.K., Srivastava, G.C., Agarwal, S., Meena, R.C.: Differences in antioxidant activity in response to salinity stress in tolerant and susceptible wheat genotypes. - Biol. Plant. 49: 85-91, 2005. Go to original source...
    34. Shanker, A.K., Djanaguiraman, M., Sudhagar, R., Chandrashekar, C.N., Pathmanabhan, G.: Differential antioxidative response of ascorbate glutathione pathway enzymes and metabolites to chromium speciation stress in green gram (Vigna radiata (L.) R.Wilczek. cv. CO4) roots. - Plant Sci. 166: 1035-1043, 2004. Go to original source...
    35. Shigeoka, S., Ishikawa, T., Tamoi, M., Miyagawa, Y., Takeda, T., Yabuta, Y., Yoshimura, K.: Regulation and function of ascorbate peroxidase isoenzymes. - J. exp. Bot. 53: 1305-1319, 2002. Go to original source...
    36. Shim, I.S., Momose, Y., Yamamoto, A., Kim, D.W., Usui, K.: Inhibition of catalase activity by oxidative stress and its relationship to salicylic acid accumulation in plants. - Plant Growth Regul. 39: 285-292, 2003. Go to original source...
    37. Silveira, J.A.G., Melo, A.R.B., Viegas, R.A., Oliveira, J.T.A.: Salinity-induced effects on nitrogen assimilation related to growth in cowpea plants. - Environ. exp. Bot. 46: 171-179, 2001. Go to original source...
    38. Sreenivasulu, N., Grimm, B., Wobus, U., Weschke, W.: Differential response of antioxidant compounds to salinity stress in salt-tolerant and salt-sensitive seedlings of foxtail millet (Setaria italica). - Physiol. Plant. 109: 435-442, 2000. Go to original source...
    39. Urbanek, H., Kuzniak - Gebarowska, E., Herka, K.: Elicitation of defense responses in bean-leaves by Botrytis cinerea polygalacturonase. - Acta Physiol. Plant. 13: 43-50, 1991.
    40. Vaidyanathan, H., Sivakumar, P., Chakrabarty, R., Thomas, G.: Scavenging of reactive oxygen species in NaCl-stressed rice (Oryza sativa L.) - differential response in salt-tolerant and sensitive varieties. - Plant Sci. 165: 1411-1418, 2003. Go to original source...
    41. Van Rossum, M.W.P.C., Alberda, M., van der Plas, L.H.W.: Role of oxidative damage in tulip bulb scale micropropagation. - Plant Sci. 130: 207-216, 1997. Go to original source...

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