Biologia plantarum 54:169-172, 2010 | DOI: 10.1007/s10535-010-0028-9

Genetic relatedness in different Medicago sativa lines based on polymorphism of mitochondrial DNA specific sequences

L. Skuza1,*, S. M. Dyba2, S. M. Rogalska1
1 University of Szczecin, Szczecin, Poland
2 Agricultural University, Poznań, Poland

Two synthetic populations (Syn-5 and Syn-7), four inbred lines and four landraces of alfalfa (Medicago sativa L.) were studied for genetic relatedness. These forms were characterized by different amount of seeds set and green mass yield. Two primer pairs of specific nucleotide sequences of mitochondrial DNA isolated from soybean were used. The mtDNA revealed the existence of significant polymorphism among the investigated forms of alfalfa. The genetic similarity (Dice coefficient) among studied forms of alfalfa ranged from 20.1 to 96.1. The greatest resemblances were noticed between D5 inbred line and the population of Syn-5. The lowest resemblances were noticed between Syn-7 and E1/2. The UPGMA dendrogram split investigated forms of alfalfa into two groups: first group include three landraces, the second consist of the rest analyzed forms. There are two landraces distinct with the highest seed set and yield of green mass: one in the first group, another one in the second group.

Keywords: alfalfa; green mass yield; mtDNA; seed yield; UPGMA
Subjects: alfalfa; genetic diversity; growth and growth analysis; Medicago sativa; mitochondrial DNA sequences; UPGMA dengrogram

Received: July 7, 2008; Accepted: December 15, 2008; Published: March 1, 2010Show citation

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Skuza, L., Dyba, S.M., & Rogalska, S.M. (2010). Genetic relatedness in different Medicago sativa lines based on polymorphism of mitochondrial DNA specific sequences. Biologia plantarum, 54(1), 169-172. doi: 10.1007/s10535-010-0028-9.
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