Biologia plantarum 56:261-268, 2012 | DOI: 10.1007/s10535-012-0085-3

Isolation and functional analysis of 4-coumarate:coenzyme A ligase gene promoters from Salvia miltiorrhiza

X. Q. Jin1, Z. W. Chen1, R. H. Tan1, S. J. Zhao1,*, Z. B. Hu1
1 Institute of Chinese Materia Medica and University of Traditional Chinese Medicine, Shanghai, P.R. China

The enzyme 4-coumarate:coenzyme A ligase (4CL) plays an important role in phenylpropanoid metabolism. The 5'-upstream regions of two Sm4CL genes were isolated from danshen (Salvia miltiorrhiza Bunge) and their functions were characterized by promoter-directed GUS gene expression assay in transgenic Arabidopsis. Seedlings containing pSm4CL1 promoter:GUS fusions showed apparent GUS staining in hypocotyl and those harboring pSm4CL2 promoter:GUS fusions were clearly stained in cotyledon vasculars and roots. Mature Arabidopsis transformed with pSm4CL1 promoter:GUS exhibited GUS expression which was weak in the shoots and scarcely in roots and those modified with pSm4CL2 promoter:GUS displayed obvious GUS staining in roots, stigmatic papillae, stamens and sepal veins. Semi-quantitative RT-PCR revealed that Sm4CL2 was transcribed at the highest level in roots which was also shown to be the major accumulation site of salvianolic acid B. The results suggested that Sm4CL2 rather than Sm4CL1 might be responsible for the biosynthesis of salvianolic acid B in danshen roots.

Keywords: danshen; GUS staining; HPLC; salvianolic acid B
Subjects: GUS staining; HPLC; salvianolic acid B; 4-coumarate: coenzyme A ligase; gene promoters; phenylpropanoid metabolites; irradiance; PCR; rosmarinic acid; jasmonates; wounding
Species: Salvia miltiorrhiza; Arabidopsis thaliana

Received: September 20, 2010; Accepted: December 20, 2010; Published: June 1, 2012Show citation

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Jin, X.Q., Chen, Z.W., Tan, R.H., Zhao, S.J., & Hu, Z.B. (2012). Isolation and functional analysis of 4-coumarate:coenzyme A ligase gene promoters from Salvia miltiorrhiza. Biologia plantarum, 56(2), 261-268. doi: 10.1007/s10535-012-0085-3.
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