Biologia plantarum 2012, 56:269-275 | DOI: 10.1007/s10535-012-0086-2

Analysis of DNA methylation during the germination of wheat seeds

F. -R. Meng1, Y. -C. Li2,3,*, J. Yin2,3, H. Liu1, X. -J. Chen1, Z. -F. Ni4, Q. -X. Sun4
1 College of Life Science, Henan Agricultural University, Zhengzhou, P.R. China
2 National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou, P.R. China
3 State Key Laboratory Cultivation Base of Crop Physiological Ecology and Genetic Improvement in Henan Province, Henan Agricultural University, Zhengzhou, P.R. China
4 Department of Plant Genetics and Breeding, State Key Lab for Agro-Biotechnology, China Agricultural University, Beijing, P.R. China

DNA methylation is known to play a crucial role in regulating plant development and organ or tissue differentiation. Here, we focused on the DNA methylation dynamics during the germination of wheat seeds using the adapted AFLP technique so called methylation-sensitive amplified polymorphism (MSAP). The MSAP profiles of genomic DNA in embryo and endosperm tissues of germinating seeds, as well as dry seeds were characterized and notable changes of cytosine methylation were detected. Comparisons of MSAP profiles in different tissues tested showed that the methylation level in dry seeds is the highest. The alteration analysis of cytosine methylation displayed that the number of demethylation events were three times higher than that of de novo methylation, which indicated that the demethylation was predominant in germinating wheat seeds, though the methylation events occurred as well. Sixteen differentially displayed DNA fragments in MSAP profiles were cloned and the sequencing analysis confirmed that nine of them contained CCGG sites. The further BLAST search showed that four of the cloned sequences were located in coding regions. Interestingly, three of the sixteen candidates were homologous to retrotransposons, which indicated that switches between DNA methylation and demethylation occurred in retrotransposon elements along with the germination of wheat seeds.

Keywords: embryo; endosperm; cloning; sequence analysis
Subjects: embryo; endosperm; cloning; sequence analysis; DNA methylation; germination; plumule; radicle; MSAP analysis
Species: Triticum aestivum

Received: June 14, 2010; Accepted: January 7, 2011; Published: June 1, 2012Show citation

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Meng, F.-R., Li, Y.-C., Yin, J., Liu, H., Chen, X.-J., Ni, Z.-F., & Sun, Q.-X. (2012). Analysis of DNA methylation during the germination of wheat seeds. Biologia plantarum, 56(2), 269-275. doi: 10.1007/s10535-012-0086-2.
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